DB code: S00252

CATH domain 3.30.1330.40 : 60s Ribosomal Protein L30; Chain Catalytic domain
E.C. 5.4.99.5
CSA 1dbf
M-CSA 1dbf
MACiE

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P19080 Chorismate mutase
CM
EC 5.4.99.5
NP_390150.1 (Protein)
NC_000964.3 (DNA/RNA sequence)
PF07736 (CM_1)
[Graphical View]
Q84FH6
Chorismate mutase
EC 5.4.99.5
PF07736 (CM_1)
[Graphical View]

KEGG enzyme name
chorismate mutase
hydroxyphenylpyruvate synthase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P19080 CHMU_BACSU Chorismate = prephenate. Homotrimer. Cytoplasm.
Q84FH6 Q84FH6_THETH

KEGG Pathways
Map code Pathways E.C.
MAP00400 Phenylalanine, tyrosine and tryptophan biosynthesis

Compound table
Substrates Products Intermediates
KEGG-id C00251 C00254
E.C.
Compound Chorismate Prephenate
Type carbohydrate,carboxyl group carbohydrate,carboxyl group
ChEBI 17333
17333
84387
84387
PubChem 12039
12039
1comA Unbound Bound:PRE Unbound
1comB Unbound Bound:PRE Unbound
1comC Unbound Bound:PRE Unbound
1comD Unbound Bound:PRE Unbound
1comE Unbound Bound:PRE Unbound
1comF Unbound Bound:PRE Unbound
1comG Unbound Bound:PRE Unbound
1comH Unbound Bound:PRE Unbound
1comI Unbound Unbound Unbound
1comJ Unbound Unbound Unbound
1comK Unbound Bound:PRE Unbound
1comL Unbound Unbound Unbound
1dbfA Unbound Unbound Unbound
1dbfB Unbound Unbound Unbound
1dbfC Unbound Unbound Unbound
1fnjA Unbound Unbound Unbound
1fnkA Unbound Unbound Unbound
2chsA Unbound Unbound Unbound
2chsB Unbound Unbound Unbound
2chsC Unbound Unbound Unbound
2chsD Unbound Unbound Unbound
2chsE Unbound Unbound Unbound
2chsF Unbound Unbound Unbound
2chsG Unbound Unbound Unbound
2chsH Unbound Unbound Unbound
2chsI Unbound Unbound Unbound
2chsJ Unbound Unbound Unbound
2chsK Unbound Unbound Unbound
2chsL Unbound Unbound Unbound
2chtA Unbound Unbound Transition-state-analogue:TSA
2chtB Unbound Unbound Transition-state-analogue:TSA
2chtC Unbound Unbound Transition-state-analogue:TSA
2chtD Unbound Unbound Transition-state-analogue:TSA
2chtE Unbound Unbound Transition-state-analogue:TSA
2chtF Unbound Unbound Transition-state-analogue:TSA
2chtG Unbound Unbound Transition-state-analogue:TSA
2chtH Unbound Unbound Transition-state-analogue:TSA
2chtI Unbound Unbound Transition-state-analogue:TSA
2chtJ Unbound Unbound Transition-state-analogue:TSA
2chtK Unbound Unbound Transition-state-analogue:TSA
2chtL Unbound Unbound Transition-state-analogue:TSA
1odeA Unbound Unbound Unbound
1odeB Unbound Unbound Unbound
1odeC Unbound Unbound Unbound
1ufyA Analogue:MLI Unbound Unbound
1ui9A Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
literature [5], [12], [14]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1comA ARG 90
1comB ARG 90
1comC ARG 90
1comD ARG 90
1comE ARG 90
1comF ARG 90
1comG ARG 90
1comH ARG 90
1comI ARG 90
1comJ ARG 90
1comK ARG 90
1comL ARG 90
1dbfA ARG 90
1dbfB ARG 90
1dbfC ARG 90
1fnjA mutant C88S, R90K
1fnkA mutant C88K, R90S
2chsA ARG 90
2chsB ARG 90
2chsC ARG 90
2chsD ARG 90
2chsE ARG 90
2chsF ARG 90
2chsG ARG 90
2chsH ARG 90
2chsI ARG 90
2chsJ ARG 90
2chsK ARG 90
2chsL ARG 90
2chtA ARG 90
2chtB ARG 90
2chtC ARG 90
2chtD ARG 90
2chtE ARG 90
2chtF ARG 90
2chtG ARG 90
2chtH ARG 90
2chtI ARG 90
2chtJ ARG 90
2chtK ARG 90
2chtL ARG 90
1odeA ARG 89
1odeB ARG 89
1odeC ARG 89
1ufyA ARG 89
1ui9A ARG 89

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[3]
1FIG.1, p.8602
[5]
Fig.1, p.494-498
[6]
Fig.1, Fig.6, Fig.7, p.650-651
[7]
Fig.1, Fig.5, Fig.6, p.200-201
[9]
Fig.1, Fig.2, p.5047-5048
[12]
p.678-679, p.681-682
[15]
Fig.1, Fig.4, p.36836-36838
[16]
Scheme 1
[20]
Fig.1
[21]
Fig.2
[22]
Scheme 1
[23]
Scheme 1
[24]
Fig.1, p.3206
[25]
p.5598
[27]
Scheme 1
[28]
Scheme 1

References
[1]
Resource
Comments
Medline ID
PubMed ID 2125470
Journal Biochemistry
Year 1990
Volume 29
Pages 8872-8
Authors Gray JV, Eren D, Knowles JR
Title Monofunctional chorismate mutase from Bacillus subtilis: kinetic and 13C NMR studies on the interactions of the enzyme with its ligands.
Related PDB
Related UniProtKB
[2]
Resource
Comments STRUCTURE BY NMR.
Medline ID 93229495
PubMed ID 8471608
Journal Biochemistry
Year 1993
Volume 32
Pages 3965-72
Authors Rajagopalan JS, Taylor KM, Jaffe EK
Title 13C NMR studies of the enzyme-product complex of Bacillus subtilis chorismate mutase.
Related PDB
Related UniProtKB P19080
[3]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
Medline ID 93391402
PubMed ID 8378335
Journal Proc Natl Acad Sci U S A
Year 1993
Volume 90
Pages 8600-3
Authors Chook YM, Ke H, Lipscomb WN
Title Crystal structures of the monofunctional chorismate mutase from Bacillus subtilis and its complex with a transition state analog.
Related PDB 2chs 2cht
Related UniProtKB P19080
[4]
Resource
Comments
Medline ID
PubMed ID 8061004
Journal Biochemistry
Year 1994
Volume 33
Pages 9953-9
Authors Gray JV, Knowles JR
Title Monofunctional chorismate mutase from Bacillus subtilis: FTIR studies and the mechanism of action of the enzyme.
Related PDB
Related UniProtKB
[5]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
Medline ID 94322390
PubMed ID 8046752
Journal J Mol Biol
Year 1994
Volume 240
Pages 476-500
Authors Chook YM, Gray JV, Ke H, Lipscomb WN
Title The monofunctional chorismate mutase from Bacillus subtilis. Structure determination of chorismate mutase and its complexes with a transition state analog and prephenate, and implications for the mechanism of the enzymatic reaction.
Related PDB 1com
Related UniProtKB P19080
[6]
Resource
Comments
Medline ID
PubMed ID 8303271
Journal Science
Year 1994
Volume 263
Pages 646-52
Authors Haynes MR, Stura EA, Hilvert D, Wilson IA
Title Routes to catalysis: structure of a catalytic antibody and comparison with its natural counterpart.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 9383421
Journal Chem Biol
Year 1995
Volume 2
Pages 195-203
Authors Lee AY, Stewart JD, Clardy J, Ganem B
Title New insight into the catalytic mechanism of chorismate mutases from structural studies.
Related PDB
Related UniProtKB
[8]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-109
Medline ID
PubMed ID
Journal J Am Chem Soc
Year 1995
Volume 117
Pages 3627-8
Authors Lee AY, Karplus PA, Ganem B, Clardy J
Title Atomic structure of the buried catalytic pocket of Escherichia coli horismate mutase.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 8643526
Journal Proc Natl Acad Sci U S A
Year 1996
Volume 93
Pages 5043-8
Authors Kast P, Asif-Ullah M, Jiang N, Hilvert D
Title Exploring the active site of chorismate mutase by combinatorial mutagenesis and selection: the importance of electrostatic catalysis.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 9384560
Journal Structure
Year 1997
Volume 5
Pages 1437-52
Authors Strater N, Schnappauf G, Braus G, Lipscomb WN
Title Mechanisms of catalysis and allosteric regulation of yeast chorismate mutase from crystal structures.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 10103029
Journal Eur J Biochem
Year 1999
Volume 261
Pages 25-32
Authors Mattei P, Kast P, Hilvert D
Title Bacillus subtilis chorismate mutase is partially diffusion-controlled.
Related PDB
Related UniProtKB
[12]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS).
Medline ID 20280181
PubMed ID 10818343
Journal Acta Crystallogr D Biol Crystallogr
Year 2000
Volume 56
Pages 673-83
Authors Ladner JE, Reddy P, Davis A, Tordova M, Howard AJ, Gilliland GL
Title The 1.30 A resolution structure of the Bacillus subtilis chorismate mutase catalytic homotrimer.
Related PDB 1dbf
Related UniProtKB P19080
[13]
Resource
Comments
Medline ID
PubMed ID 11087356
Journal Biochemistry
Year 2000
Volume 39
Pages 14087-94
Authors Gamper M, Hilvert D, Kast P
Title Probing the role of the C-terminus of Bacillus subtilis chorismate mutase by a novel random protein-termination strategy.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 10815997
Journal Comput Chem
Year 2000
Volume 24
Pages 275-85
Authors Worthington SE, Krauss M
Title Effective fragment potentials and the enzyme active site.
Related PDB
Related UniProtKB
[15]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10960481
Journal J Biol Chem
Year 2000
Volume 275
Pages 36832-8
Authors Kast P, Grisostomi C, Chen IA, Li S, Krengel U, Xue Y, Hilvert D
Title A strategically positioned cation is crucial for efficient catalysis by chorismate mutase.
Related PDB 1fnj 1fnk
Related UniProtKB
[16]
Resource
Comments
Medline ID
PubMed ID 11456771
Journal J Am Chem Soc
Year 2001
Volume 123
Pages 1709-12
Authors Marti S, Andres J, Moliner V, Silla E, Tunon I, Bertran J, Field MJ
Title A hybrid potential reaction path and free energy study of the chorismate mutase reaction.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID 11495249
Journal J Biomol NMR
Year 2001
Volume 20
Pages 177-80
Authors Eletsky A, Kienhofer A, Pervushin K
Title TROSY NMR with partially deuterated proteins.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 12392438
Journal J Am Chem Soc
Year 2002
Volume 124
Pages 12898-902
Authors Pervushin K, Vogeli B, Eletsky A
Title Longitudinal (1)H relaxation optimization in TROSY NMR spectroscopy.
Related PDB
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 12449416
Journal J Biomol NMR
Year 2002
Volume 24
Pages 31-9
Authors Eletsky A, Heinz T, Moreira O, Kienhofer A, Hilvert D, Pervushi K
Title Direct NMR observation and DFT calculations of a hydrogen bond at the active site of a 44 kDa enzyme.
Related PDB
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 12698486
Journal Angew Chem Int Ed Engl
Year 2003
Volume 42
Pages 1508-11
Authors Guo H, Cui Q, Lipscomb WN, Karplus M
Title Understanding the role of active-site residues in chorismate mutase catalysis from molecular-dynamics simulations.
Related PDB
Related UniProtKB
[21]
Resource
Comments
Medline ID
PubMed ID 12584715
Journal Chemistry
Year 2003
Volume 9
Pages 984-91
Authors Marti S, Andres J, Moliner V, Silla E, Tunon I, Bertran J
Title Preorganization and reorganization as related factors in enzyme catalysis: the chorismate mutase case.
Related PDB
Related UniProtKB
[22]
Resource
Comments
Medline ID
PubMed ID 12783541
Journal J Am Chem Soc
Year 2003
Volume 125
Pages 6892-9
Authors Guimaraes CR, Repasky MP, Chandrasekhar J, Tirado-Rives J, Jorgensen WL
Title Contributions of conformational compression and preferential transition state stabilization to the rate enhancement by chorismate mutase.
Related PDB
Related UniProtKB
[23]
Resource
Comments
Medline ID
PubMed ID 12940735
Journal J Am Chem Soc
Year 2003
Volume 125
Pages 10540-2
Authors Hur S, Bruice TC
Title Just a near attack conformer for catalysis (chorismate to prephenate rearrangements in water, antibody, enzymes, and their mutants).
Related PDB
Related UniProtKB
[24]
Resource
Comments
Medline ID
PubMed ID 12630863
Journal J Am Chem Soc
Year 2003
Volume 125
Pages 3206-7
Authors Kienhofer A, Kast P, Hilvert D
Title Selective stabilization of the chorismate mutase transition state by a positively charged hydrogen bond donor.
Related PDB
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID 12733875
Journal J Am Chem Soc
Year 2003
Volume 125
Pages 5598-9
Authors Mandal A, Hilvert D
Title Charge optimization increases the potency and selectivity of a chorismate mutase inhibitor.
Related PDB
Related UniProtKB
[26]
Resource
Comments
Medline ID
PubMed ID 12766412
Journal J Biomol NMR
Year 2003
Volume 26
Pages 167-79
Authors Eletsky A, Moreira O, Kovacs H, Pervushin K
Title A novel strategy for the assignment of side-chain resonances in completely deuterated large proteins using 13C spectroscopy.
Related PDB
Related UniProtKB
[27]
Resource
Comments
Medline ID
PubMed ID 12926249
Journal Org Biomol Chem
Year 2003
Volume 1
Pages 483-7
Authors Marti S, Moliner V, Tunon I, Williams IH
Title QM/MM calculations of kinetic isotope effects in the chorismate mutase active site.
Related PDB
Related UniProtKB
[28]
Resource
Comments
Medline ID
PubMed ID 14523243
Journal Proc Natl Acad Sci U S A
Year 2003
Volume 100
Pages 12015-20
Authors Hur S, Bruice TC
Title The near attack conformation approach to the study of the chorismate to prephenate reaction.
Related PDB
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID 14727008
Journal Arch Microbiol
Year 2004
Volume 181
Pages 195-203
Authors Helmstaedt K, Heinrich G, Merkl R, Braus GH
Title Chorismate mutase of Thermus thermophilus is a monofunctional AroH class enzyme inhibited by tyrosine.
Related PDB
Related UniProtKB Q84FH6
[30]
Resource
Comments
Medline ID
PubMed ID 15136856
Journal Chem Commun (Camb)
Year 2004
Volume (10)
Pages 1238-9
Authors Ranaghan KE, Mulholland AJ
Title Conformational effects in enzyme catalysis: QM/MM free energy calculation of the 'NAC' contribution in chorismate mutase.
Related PDB
Related UniProtKB
[31]
Resource
Comments
Medline ID
PubMed ID 15096202
Journal Eur J Biochem
Year 2004
Volume 271
Pages 1630-7
Authors Woycechowsky KJ, Hilvert D
Title Deciphering enzymes. Genetic selection as a probe of structure and mechanism.
Related PDB
Related UniProtKB

Comments
This enzyme catalyzes Claisen rearrangement (or concerted addition and elimination).

Created Updated
2005-07-11 2009-02-26