DB code: S00230
| RLCP classification | 3.944.220700.2 : Transfer | |
|---|---|---|
| CATH domain | 3.20.20.105 : TIM Barrel | Catalytic domain |
| E.C. | 2.4.2.29 | |
| CSA | 1pud | |
| M-CSA | 1pud | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P28720 |
Queuine tRNA-ribosyltransferase
|
EC
2.4.2.29
tRNA-guanine transglycosylase Guanine insertion enzyme |
YP_162098.2
(Protein)
NC_006526.2 (DNA/RNA sequence) |
PF01702
(TGT)
[Graphical View] |
| Q9X1P7 |
Queuine tRNA-ribosyltransferase
|
EC
2.4.2.29
tRNA-guanine transglycosylase Guanine insertion enzyme |
NP_229361.1
(Protein)
NC_000853.1 (DNA/RNA sequence) |
PF01702
(TGT)
[Graphical View] |
| KEGG enzyme name |
|---|
|
tRNA-guanine transglycosylase
guanine insertion enzyme tRNA transglycosylase Q-insertase queuine transfer ribonucleate ribosyltransferase transfer ribonucleate glycosyltransferase tRNA guanine transglycosidase guanine, queuine-tRNA transglycosylase queuine tRNA-ribosyltransferase TGT [tRNA]-guanine:queuine tRNA-D-ribosyltransferase transfer ribonucleic acid guanine transglycosylase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P28720 | TGT_ZYMMO | [tRNA]-guanine + queuine = [tRNA]-queuine + guanine. [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. | Monomer. | Binds 1 zinc ion per subunit. | |
| Q9X1P7 | TGT_THEMA | [tRNA]-guanine + queuine = [tRNA]-queuine + guanine. [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. | Binds 1 zinc ion per subunit (By similarity). |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00038 | C01977 | C01449 | C01978 | C00242 | ||||||
| E.C. | |||||||||||
| Compound | Zinc | tRNA guanine | Queuine | tRNA queuine | Guanine | enzyme-tRNA covalent intermediate | |||||
| Type | heavy metal | amide group,amine group,nucleic acids | amide group,amine group,aromatic ring (with nitrogen atoms),carbohydrate | amide group,amine group,carbohydrate,nucleic acids | amide group,amine group,aromatic ring (with nitrogen atoms) | ||||||
| ChEBI |
29105 29105 |
17433 17433 |
16235 16235 |
||||||||
| PubChem |
32051 32051 |
114881 114881 |
764 764 |
||||||||
| 1efzA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:PRF | Unbound | Unbound | Unbound |
| 1enuA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:APZ | Unbound |
| 1f3eA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:DPZ | Unbound |
| 1k4gA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:AIQ | Unbound | Unbound | Unbound |
| 1k4hA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:APQ | Unbound | Unbound | Unbound |
| 1n2vA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:BDI | Unbound | Unbound | Unbound |
| 1ozmA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1ozqA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:PRF | Unbound | Unbound | Unbound |
| 1p0bA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:PQ0 | Unbound | Unbound | Unbound |
| 1p0dA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1p0eA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:PRF | Unbound | Unbound | Unbound |
| 1pudA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1pxgA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:PRF | Unbound | Unbound | Unbound |
| 1q2rA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:9DG | Bound:ASP_280-ROB (chain E) |
| 1q2rB |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:9DG | Unbound |
| 1q2rC |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:9DG | Bound:ASP_280-ROB (chain F) |
| 1q2rD |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:9DG | Unbound |
| 1q2sA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Analogue:A-G-C-A-C-G-G-C-U-PQ1-U-A-A-A-C-C-G-U-G-C (chain E) | Unbound | Unbound |
| 1q2sB |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1q2sC |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:9DG | Analogue:ASP_280-ROB (chain F) |
| 1q2sD |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1q4wA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:DQU | Unbound |
| 1q63A |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:AIQ | Unbound | Unbound | Unbound |
| 1q65A |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:BHB | Unbound | Unbound | Unbound |
| 1q66A |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:KMB | Unbound | Unbound | Unbound |
| 1r5yA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:DQU | Unbound |
| 1s38A |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:MAQ | Unbound |
| 1s39A |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Analogue:AQO | Unbound |
| 1wkdA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1wkeA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1wkfA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1y5vA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:NE8 | Unbound | Unbound | Unbound |
| 1y5wA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:NEZ | Unbound | Unbound | Unbound |
| 1y5xA |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:E89 | Unbound | Unbound | Unbound |
| 1y5xD |
|
|
|
|
|
Bound:_ZN | Unbound | Analogue:E89 | Unbound | Unbound | Unbound |
| 2ashA |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 2ashB |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 2ashC |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| 2ashD |
|
|
|
|
|
Bound:_ZN | Unbound | Unbound | Unbound | Unbound | Unbound |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P28720 & literature [29], [31] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1efzA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | mutant S103A | ||
| 1enuA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1f3eA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1k4gA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1k4hA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1n2vA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1ozmA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | mutant Y106F | ||
| 1ozqA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | mutant Y106F | ||
| 1p0bA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1p0dA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1p0eA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1pudA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1pxgA |
|
|
|
|
|
ASP 102; | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | mutant D280E | ||
| 1q2rA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q2rB |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q2rC |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q2rD |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q2sA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q2sB |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q2sC |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q2sD |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q4wA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q63A |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q65A |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1q66A |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1r5yA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1s38A |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1s39A |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1wkdA |
|
|
|
|
|
;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | mutant D102A | ||
| 1wkeA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | mutant D156A | ||
| 1wkfA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | mutant D156Y | ||
| 1y5vA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1y5wA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1y5xA |
|
|
|
|
|
ASP 102;ASP 280 | CYS 318;CYS 320;CYS 323;HIS 349(Zinc binding) | |||
| 1y5xD |
|
|
|
|
|
ASP 1102;ASP 1280 | CYS 1318;CYS 1320;CYS 1323;HIS 1349(Zinc binding) | |||
| 2ashA |
|
|
|
|
|
ASP 89;ASP 261 | CYS 299;CYS 301;CYS 304;HIS 330(Zinc binding) | |||
| 2ashB |
|
|
|
|
|
ASP 89;ASP 261 | CYS 299;CYS 301;CYS 304;HIS 330(Zinc binding) | |||
| 2ashC |
|
|
|
|
|
ASP 89;ASP 261 | CYS 299;CYS 301;CYS 304;HIS 330(Zinc binding) | |||
| 2ashD |
|
|
|
|
|
ASP 89;ASP 261 | CYS 299;CYS 301;CYS 304;HIS 330(Zinc binding) | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[15]
|
Fig.4, p.15738 | |
|
[16]
|
p.2853-2855 | |
|
[21]
|
Fig.4, p.145 | |
|
[22]
|
Fig.2, p.14128-14132 | |
|
[27]
|
Fig.1, p.1072-1074 | |
|
[29]
|
Fig.2, Fig.4, Fig.7, p.42376 | |
|
[31]
|
Fig.1, p.772 | |
|
[32]
|
p.786-787 | |
|
[33]
|
Fig.2, p.56 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6696916 |
| Journal | Biochim Biophys Acta |
| Year | 1984 |
| Volume | 781 |
| Pages | 64-75 |
| Authors | Farkas WR, Jacobson KB, Katze JR |
| Title | Substrate and inhibitor specificity of tRNA-guanine ribosyltransferase |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3117541 |
| Journal | Eur J Biochem |
| Year | 1987 |
| Volume | 168 |
| Pages | 219-25 |
| Authors | Haumont E, Droogmans L, Grosjean H |
| Title |
Enzymatic formation of queuosine and of glycosyl queuosine in yeast tRNAs microinjected into Xenopus laevis oocytes. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8494901 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 5239-46 |
| Authors | Curnow AW, Kung FL, Koch KA, Garcia GA |
| Title | tRNA-guanine transglycosylase from Escherichia coli |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8323579 |
| Journal | J Mol Biol |
| Year | 1993 |
| Volume | 231 |
| Pages | 489-97 |
| Authors | Garcia GA, Koch KA, Chong S |
| Title |
tRNA-guanine transglycosylase from Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8003468 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 7041-6 |
| Authors | Reuter K, Chong S, Ullrich F, Kersten H, Garcia GA |
| Title | Serine 90 is required for enzymic activity by tRNA-guanine transglycosylase from Escherichia coli |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7748954 |
| Journal | Biochimie |
| Year | 1994 |
| Volume | 76 |
| Pages | 1183-91 |
| Authors | Curnow AW, Garcia GA |
| Title | tRNA-guanine transglycosylase from Escherichia coli |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7528209 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 32221-5 |
| Authors | Nakanishi S, Ueda T, Hori H, Yamazaki N, Okada N, Watanabe K |
| Title | A UGU sequence in the anticodon loop is a minimum requirement for recognition by Escherichia coli tRNA-guanine transglycosylase |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7893665 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 3694-701 |
| Authors | Chong S, Curnow AW, Huston TJ, Garcia GA |
| Title |
tRNA-guanine transglycosylase from Escherichia coli is a zinc metalloprotein. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7492556 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 15539-44 |
| Authors | Hoops GC, Townsend LB, Garcia GA |
| Title | Mechanism-based inactivation of tRNA-guanine transglycosylase from Escherichia coli by 2-amino-5-(fluoromethyl)pyrrolo[2,3-d]pyrimidin-4 (3H)-one |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7578154 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 15381-7 |
| Authors | Hoops GC, Townsend LB, Garcia GA |
| Title | tRNA-guanine transglycosylase from Escherichia coli |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7665516 |
| Journal | J Bacteriol |
| Year | 1995 |
| Volume | 177 |
| Pages | 5284-8 |
| Authors | Reuter K, Ficner R |
| Title | Sequence analysis and overexpression of the Zymomonas mobilis tgt gene encoding tRNA-guanine transglycosylase |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7615526 |
| Journal | J Biol Chem |
| Year | 1995 |
| Volume | 270 |
| Pages | 17264-7 |
| Authors | Curnow AW, Garcia GA |
| Title |
tRNA-guanine transglycosylase from Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8579355 |
| Journal | Arch Biochem Biophys |
| Year | 1996 |
| Volume | 326 |
| Pages | 1-7 |
| Authors | Deshpande KL, Seubert PH, Tillman DM, Farkas WR, Katze JR |
| Title | Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8608154 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 3133-9 |
| Authors | Garcia GA, Tierney DL, Chong S, Clark K, Penner-Hahn JE |
| Title | X-ray absorption spectroscopy of the zinc site in tRNA-guanine transglycosylase from Escherichia coli |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8961936 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 15734-9 |
| Authors | Romier C, Reuter K, Suck D, Ficner R |
| Title | Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase reveal aspartate 102 as the active site nucleophile |
| Related PDB | 1wkd 1wke 1wkf |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS). |
| Medline ID | 96256303 |
| PubMed ID | 8654383 |
| Journal | EMBO J |
| Year | 1996 |
| Volume | 15 |
| Pages | 2850-7 |
| Authors | Romier C, Reuter K, Suck D, Ficner R |
| Title | Crystal structure of tRNA-guanine transglycosylase |
| Related PDB | 1pud |
| Related UniProtKB | P28720 |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8860000 |
| Journal | Proteins |
| Year | 1996 |
| Volume | 24 |
| Pages | 516-9 |
| Authors | Romier C, Ficner R, Reuter K, Suck D |
| Title |
Purification, |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9369241 |
| Journal | FEBS Lett |
| Year | 1997 |
| Volume | 416 |
| Pages | 93-8 |
| Authors | Romier C, Meyer JE, Suck D |
| Title | Slight sequence variations of a common fold explain the substrate specificities of tRNA-guanine transglycosylases from the three kingdoms |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9055203 |
| Journal | J Protein Chem |
| Year | 1997 |
| Volume | 16 |
| Pages | 11-7 |
| Authors | Garcia GA, Chong S |
| Title |
Cysteine 265 is in the active site of, |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9403840 |
| Journal | Med Microbiol Immunol (Berl) |
| Year | 1997 |
| Volume | 186 |
| Pages | 125-34 |
| Authors | Bereswill S, Fassbinder F, Volzing C, Haas R, Reuter K, Ficner R, Kist M |
| Title | Cloning and functional characterization of the genes encoding 3-dehydroquinate synthase (aroB) and tRNA-guanine transglycosylase (tgt) from Helicobacter pylori |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments |
X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 10-381, |
| Medline ID | |
| PubMed ID | 10413112 |
| Journal | FEBS Lett |
| Year | 1999 |
| Volume | 454 |
| Pages | 142-6 |
| Authors | Gradler U, Ficner R, Garcia GA, Stubbs MT, Klebe G, Reuter K |
| Title | Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity |
| Related PDB | 1efz |
| Related UniProtKB | P28720 |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11714265 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 14123-33 |
| Authors | Kittendorf JD, Barcomb LM, Nonekowski ST, Garcia GA |
| Title | tRNA-guanine transglycosylase from Escherichia coli |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11255023 |
| Journal | Gene |
| Year | 2001 |
| Volume | 265 |
| Pages | 205-12 |
| Authors | Deshpande KL, Katze JR |
| Title | Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS). |
| Medline ID | 21103718 |
| PubMed ID | 11178905 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 306 |
| Pages | 455-67 |
| Authors | Gradler U, Gerber HD, Goodenough-Lashua DM, Garcia GA, Ficner R, Reuter K, Stubbs MT, Klebe G |
| Title | A new target for shigellosis |
| Related PDB | 1f3e |
| Related UniProtKB | P28720 |
| [25] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 11-385. |
| Medline ID | |
| PubMed ID | 11921407 |
| Journal | Chembiochem |
| Year | 2002 |
| Volume | 3 |
| Pages | 250-3 |
| Authors | Meyer EA, Brenk R, Castellano RK, Furler M, Klebe G, Diederich F |
| Title |
De novo design, |
| Related PDB | 1k4g 1k4h |
| Related UniProtKB | P28720 |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11751936 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 7178-82 |
| Authors | Nonekowski ST, Kung FL, Garcia GA |
| Title | The Escherichia coli tRNA-guanine transglycosylase can recognize and modify DNA |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 14523925 |
| Journal | Chembiochem |
| Year | 2003 |
| Volume | 4 |
| Pages | 1066-77 |
| Authors | Brenk R, Stubbs MT, Heine A, Reuter K, Klebe G |
| Title |
Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, |
| Related PDB | 1ozm 1ozq 1p0b 1p0d 1p0e |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12581659 |
| Journal | Curr Opin Struct Biol |
| Year | 2003 |
| Volume | 13 |
| Pages | 49-55 |
| Authors | Ferre-D'Amare AR |
| Title | RNA-modifying enzymes. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12909636 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 42369-76 |
| Authors | Kittendorf JD, Sgraja T, Reuter K, Klebe G, Garcia GA |
| Title | An essential role for aspartate 264 in catalysis by tRNA-guanine transglycosylase from Escherichia coli. |
| Related PDB | 1pxg |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 11-385. |
| Medline ID | |
| PubMed ID | 12646024 |
| Journal | J Med Chem |
| Year | 2003 |
| Volume | 46 |
| Pages | 1133-43 |
| Authors | Brenk R, Naerum L, Gradler U, Gerber HD, Garcia GA, Reuter K, Stubbs MT, Klebe G |
| Title | Virtual screening for submicromolar leads of tRNA-guanine transglycosylase based on a new unexpected binding mode detected by crystal structure analysis. |
| Related PDB | 1n2v |
| Related UniProtKB | P28720 |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14513020 |
| Journal | Nat Struct Biol |
| Year | 2003 |
| Volume | 10 |
| Pages | 772-3 |
| Authors | Correll CC |
| Title | Caught in the act of modifying tRNA. |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12949492 |
| Journal | Nat Struct Biol |
| Year | 2003 |
| Volume | 10 |
| Pages | 781-8 |
| Authors | Xie W, Liu X, Huang RH |
| Title | Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate. |
| Related PDB | 1q2r 1q2s |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15050823 |
| Journal | J Mol Biol |
| Year | 2004 |
| Volume | 338 |
| Pages | 55-75 |
| Authors | Brenk R, Meyer EA, Reuter K, Stubbs MT, Garcia GA, Diederich F, Klebe G |
| Title | Crystallographic study of inhibitors of tRNA-guanine transglycosylase suggests a new structure-based pharmacophore for virtual screening. |
| Related PDB | 1q4w 1q63 1q65 1q66 1r5y |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | |
| Journal | Helv Chim Acta |
| Year | 2004 |
| Volume | 87 |
| Pages | 1333-56g |
| Authors | Meyer EA, Furler M, Diederich F, Brenk R, Klebe G |
| Title | Synthesis and in vitro evaluation of 2-aminoquinazolin-4(3h)-one-based inhibitors for tRNA-guanine transglycosylase (tgt). |
| Related PDB | 1s38 1s39 |
| Related UniProtKB | |
| Comments |
|---|
|
Although this enzyme binds a zinc ion, According to the literature [31] and [32], (1) Asp280 makes a nucleophilic attack on the C1' atom of tRNA ribose, (2) Asp102 acts as a general base activate the N9 atom of queine(or preQ1), (3) The activated queine (or preQ1) makes a nucleophilic attack on the C1' atom of the intermediate, |
| Created | Updated |
|---|---|
| 2005-03-29 | 2009-02-26 |