DB code: D00499
| CATH domain | 2.70.100.10 : 1,4-Beta-D-Glucan Cellobiohydrolase I; Chain A | Catalytic domain |
|---|---|---|
| 2.-.-.- : | ||
| E.C. | 3.2.1.91 | |
| CSA | 1cel | |
| M-CSA | 1cel | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 2.70.100.10 : 1,4-Beta-D-Glucan Cellobiohydrolase I; Chain A | S00162 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | CAZy | Pfam |
|---|---|---|---|---|
| P62694 |
Exoglucanase 1
|
EC
3.2.1.91
Exoglucanase I Exocellobiohydrolase I CBHI 1,4-beta-cellobiohydrolase |
CBM1
(Carbohydrate-Binding Module Family 1)
GH7 (Glycoside Hydrolase Family 7) |
PF00734
(CBM_1)
PF00840 (Glyco_hydro_7) [Graphical View] |
| Q09431 |
|
Cellulase
EC 3.2.1.91 |
CBM1
(Carbohydrate-Binding Module Family 1)
GH7 (Glycoside Hydrolase Family 7) |
PF00734
(CBM_1)
PF00840 (Glyco_hydro_7) [Graphical View] |
| Q7LIJ0 |
|
Major cellobiohydrolase (Cellulase)
EC 3.2.1.91 |
PF00734
(CBM_1)
PF00840 (Glyco_hydro_7) [Graphical View] |
|
| Q8TFL9 |
|
Cellobiohydrolase I catalytic domain (Cellobiohydrolase 1 catalytic domain)
EC 3.2.1.91 |
GH7
(Glycoside Hydrolase Family 7)
|
PF00840
(Glyco_hydro_7)
[Graphical View] |
| KEGG enzyme name |
|---|
|
cellulose 1,4-beta-cellobiosidase
exo-cellobiohydrolase beta-1,4-glucan cellobiohydrolase beta-1,4-glucan cellobiosylhydrolase 1,4-beta-glucan cellobiosidase exoglucanase avicelase CBH 1 C1 cellulase cellobiohydrolase I cellobiohydrolase exo-beta-1,4-glucan cellobiohydrolase 1,4-beta-D-glucan cellobiohydrolase cellobiosidase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P62694 | GUX1_TRIRE | Hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non- reducing ends of the chains. | Secreted. | ||
| Q09431 | Q09431_PHACH | ||||
| Q7LIJ0 | Q7LIJ0_PHACH | ||||
| Q8TFL9 | Q8TFL9_TALEM |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00500 | Starch and sucrose metabolism |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||
| KEGG-id | C00760 | C02013 | C00001 | C00185 | C00760 | ||||||
| E.C. | |||||||||||
| Compound | Cellulose | Cellotetraose | H2O | Cellobiose | Cellulose | ||||||
| Type | polysaccharide | polysaccharide | H2O | polysaccharide | polysaccharide | ||||||
| ChEBI |
62974 62974 |
15377 15377 |
17057 17057 |
||||||||
| PubChem |
439626 439626 |
22247451 962 22247451 962 |
439178 439178 |
||||||||
| 1celA |
|
|
|
|
|
Unbound | Unbound | Analogue:IBZ-GLC | Unbound | ||
| 1celB |
|
|
|
|
|
Unbound | Unbound | Analogue:IBZ-GLC | Unbound | ||
| 1dy4A |
|
|
|
|
|
Unbound | Unbound | Analogue:SNP | Unbound | ||
| 1egnA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 1q2bA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 1q2eA |
|
|
|
|
|
Unbound | Unbound | Analogue:MGL-SGC-GLC-GLC | Unbound | ||
| 1q2eB |
|
|
|
|
|
Unbound | Unbound | Analogue:MGL-SGC-GLC-GLC | Unbound | ||
| 2celA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 2celB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 3celA |
|
|
|
|
|
Unbound | Unbound | Bound:GLC-GLC | Unbound | ||
| 4celA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 4celB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 5celA |
|
|
|
|
|
Unbound | Bound:GLC-GLC-GLC-GLC_455 | Unbound | Unbound | ||
| 6celA |
|
|
|
|
|
Unbound | Unbound | Analogue:GLC-GLC-GLC-GLC | Bound:GLC-GLC-GLC-GLC-GLC | ||
| 7celA |
|
|
|
|
|
Unbound | Unbound | Bound:GLC-GLC | Bound:GLC-GLC-GLC-GLC-GLC-GLC | ||
| 1gpiA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 1h46X |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 1z3tA |
|
|
|
|
|
Unbound | Unbound | Bound:CBI | Unbound | ||
| 1z3vA |
|
|
|
|
|
Unbound | Unbound | Analogue:LAT | Unbound | ||
| 1z3wA |
|
|
|
|
|
Unbound | Unbound | Analogue:IDC | Unbound | ||
| 1q9hA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 1az6A |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 1azhA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 1azkA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 1cbhA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| 2cbhA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| literature [14], [18] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1celA |
|
|
|
|
|
GLU 212;ASP 214;GLU 217;HIS 228 | ||||
| 1celB |
|
|
|
|
|
GLU 212;ASP 214;GLU 217;HIS 228 | ||||
| 1dy4A |
|
|
|
|
|
GLU 212;ASP 214;GLU 217;HIS 228 | ||||
| 1egnA |
|
|
|
|
|
GLU 212;ASP 214;GLU 217;HIS 228 | mutant E223S,A224H,L225V,T226A,D262G | |||
| 1q2bA |
|
|
|
|
|
GLU 212;ASP 214;GLU 217;HIS 228 | mutant D241C,D249C | |||
| 1q2eA |
|
|
|
|
|
GLU 212;ASP 214;GLU 217;HIS 228 | mutant deletion 245-252 | |||
| 1q2eB |
|
|
|
|
|
GLU 212;ASP 214;GLU 217;HIS 228 | mutant deletion 245-252 | |||
| 2celA |
|
|
|
|
|
;ASP 214;GLU 217;HIS 228 | mutant E212Q | |||
| 2celB |
|
|
|
|
|
;ASP 214;GLU 217;HIS 228 | mutant E212Q | |||
| 3celA |
|
|
|
|
|
;ASP 214;GLU 217;HIS 228 | mutant E212Q | |||
| 4celA |
|
|
|
|
|
GLU 212;;GLU 217;HIS 228 | mutant D214N | |||
| 4celB |
|
|
|
|
|
GLU 212;;GLU 217;HIS 228 | mutant D214N | |||
| 5celA |
|
|
|
|
|
;ASP 214;GLU 217;HIS 228 | mutant E212Q | |||
| 6celA |
|
|
|
|
|
;ASP 214;GLU 217;HIS 228 | mutant E212Q | |||
| 7celA |
|
|
|
|
|
GLU 212;ASP 214;;HIS 228 | mutant E217Q | |||
| 1gpiA |
|
|
|
|
|
GLU 207;ASP 209;GLU 212;HIS 223 | ||||
| 1h46X |
|
|
|
|
|
GLU 207;ASP 209;GLU 212;HIS 223 | ||||
| 1z3tA |
|
|
|
|
|
GLU 207;ASP 209;GLU 212;HIS 223 | ||||
| 1z3vA |
|
|
|
|
|
GLU 207;ASP 209;GLU 212;HIS 223 | ||||
| 1z3wA |
|
|
|
|
|
GLU 207;ASP 209;GLU 212;HIS 223 | ||||
| 1q9hA |
|
|
|
|
|
GLU 209;ASP 211;GLU 214;HIS 225 | ||||
| 1az6A |
|
|
|
|
|
mutant Y5A,Y31A,Y32A | ||||
| 1azhA |
|
|
|
|
|
mutant Y5A,Y31A,Y32A | ||||
| 1azkA |
|
|
|
|
|
mutant Y5A,Y31A,Y32A | ||||
| 1cbhA |
|
|
|
|
|
mutant E126Q,D130N,D132A | ||||
| 2cbhA |
|
|
|
|
|
|||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[8]
|
p.887 | |
|
[14]
|
p.526 | |
|
[17]
|
p.342-344 | |
|
[21]
|
Scheme 1 | |
|
[23]
|
p.317-319 | |
|
[25]
|
Scheme 1 | |
|
[26]
|
p.1101 | |
|
[27]
|
p.88 | |
|
[34]
|
p.1960 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3178831 |
| Journal | Biochem Biophys Res Commun |
| Year | 1988 |
| Volume | 156 |
| Pages | 180-5 |
| Authors | Abuja PM, Pilz I, Claeyssens M, Tomme P |
| Title | Domain structure of cellobiohydrolase II as studied by small angle X-ray scattering: close resemblance to cellobiohydrolase I. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2597150 |
| Journal | Biochem Biophys Res Commun |
| Year | 1989 |
| Volume | 165 |
| Pages | 615-23 |
| Authors | Abuja PM, Pilz I, Tomme P, Claeyssens M |
| Title | Structural changes in cellobiohydrolase I upon binding of a macromolecular ligand as evident by SAXS investigations. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 478-513. |
| Medline ID | 90057416 |
| PubMed ID | 2554967 |
| Journal | Biochemistry |
| Year | 1989 |
| Volume | 28 |
| Pages | 7241-57 |
| Authors | Kraulis J, Clore GM, Nilges M, Jones TA, Pettersson G, Knowles J, Gronenborn AM |
| Title |
Determination of the three-dimensional solution structure of the C-terminal domain of cellobiohydrolase I from Trichoderma reesei. |
| Related PDB | 1cbh 2cbh |
| Related UniProtKB | P62694 |
| [4] | |
| Resource | |
| Comments | ACTIVE SITE |
| Medline ID | |
| PubMed ID | |
| Journal | FEBS Lett |
| Year | 1989 |
| Volume | 243 |
| Pages | 239-43 |
| Authors | Tomme P, Clayssens M |
| Title | Identification of a functionally important carboxyl group in cellobiohydrolase I from Trichoderma reesei. |
| Related PDB | |
| Related UniProtKB | P62694 |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2332056 |
| Journal | FEBS Lett |
| Year | 1990 |
| Volume | 263 |
| Pages | 89-92 |
| Authors | Claeyssens M, Tomme P, Brewer CF, Hehre EJ |
| Title | Stereochemical course of hydrolysis and hydration reactions catalysed by cellobiohydrolases I and II from Trichoderma reesei. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2261982 |
| Journal | FEBS Lett |
| Year | 1990 |
| Volume | 275 |
| Pages | 135-8 |
| Authors | Mitsuishi Y, Nitisinprasert S, Saloheimo M, Biese I, Reinikainen T, Claeyssens M, Keranen S, Knowles JK, Teeri TT |
| Title | Site-directed mutagenesis of the putative catalytic residues of Trichoderma reesei cellobiohydrolase I and endoglucanase I. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1567377 |
| Journal | Biochem J |
| Year | 1992 |
| Volume | 283 |
| Pages | 31-4 |
| Authors | Brooks MM, Tuohy MG, Savage AV, Claeyssens M, Coughlan MP |
| Title | The stereochemical course of reactions catalysed by the cellobiohydrolases produced by Talaromyces emersonii. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8489514 |
| Journal | Biochem J |
| Year | 1993 |
| Volume | 291 |
| Pages | 883-8 |
| Authors | Konstantinidis AK, Marsden I, Sinnott ML |
| Title | Hydrolyses of alpha- and beta-cellobiosyl fluorides by cellobiohydrolases of Trichoderma reesei. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8252556 |
| Journal | Carbohydr Res |
| Year | 1993 |
| Volume | 249 |
| Pages | 77-90 |
| Authors | McCarter JD, Adam MJ, Braun C, Namchuk M, Tull D, Withers SG |
| Title | Syntheses of 2-deoxy-2-fluoro mono- and oligo-saccharide glycosides from glycals and evaluation as glycosidase inhibitors. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8407900 |
| Journal | J Biol Chem |
| Year | 1993 |
| Volume | 268 |
| Pages | 20756-61 |
| Authors | Srisodsuk M, Reinikainen T, Penttila M, Teeri TT |
| Title | Role of the interdomain linker peptide of Trichoderma reesei cellobiohydrolase I in its interaction with crystalline cellulose. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8254686 |
| Journal | J Mol Biol |
| Year | 1993 |
| Volume | 234 |
| Pages | 905-7 |
| Authors | Divne C, Sinning I, Stahlberg J, Pettersson G, Bailey M, Siika-aho M, Margolles-Clark E, Teeri T, Jones TA |
| Title | Crystallization and preliminary X-ray studies on the core proteins of cellobiohydrolase I and endoglucanase I from Trichoderma reesei. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8037459 |
| Journal | Arch Biochem Biophys |
| Year | 1994 |
| Volume | 312 |
| Pages | 459-66 |
| Authors | Evans BR, Margalit R, Woodward J |
| Title | Veratryl alcohol oxidase activity of a chemically modified cellulase protein. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8192865 |
| Journal | Biotechnol Appl Biochem |
| Year | 1994 |
| Volume | 19 |
| Pages | 141-53 |
| Authors | Woodward J, Brown JP, Evans BR, Affholter KA |
| Title | Papain digestion of crude Trichoderma reesei cellulase: purification and properties of cellobiohydrolase I and II core proteins. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.81 ANGSTROMS) OF 18-452. |
| Medline ID | 94310436 |
| PubMed ID | 8036495 |
| Journal | Science |
| Year | 1994 |
| Volume | 265 |
| Pages | 524-8 |
| Authors | Divne C, Stahlberg J, Reinikainen T, Ruohonen L, Pettersson G, Knowles JK, Teeri TT, Jones TA |
| Title | The three-dimensional crystal structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei. |
| Related PDB | 1cel |
| Related UniProtKB | P62694 |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7549870 |
| Journal | Protein Sci |
| Year | 1995 |
| Volume | 4 |
| Pages | 1056-64 |
| Authors | Linder M, Mattinen ML, Kontteli M, Lindeberg G, Stahlberg J, Drakenberg T, Reinikainen T, Pettersson G, Annila A |
| Title | Identification of functionally important amino acids in the cellulose-binding domain of Trichoderma reesei cellobiohydrolase I. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8706890 |
| Journal | FEBS Lett |
| Year | 1996 |
| Volume | 390 |
| Pages | 339-44 |
| Authors | Henriksson H, Stahlberg J, Isaksson R, Pettersson G |
| Title | The active sites of cellulases are involved in chiral recognition: a comparison of cellobiohydrolase 1 and endoglucanase 1. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8951380 |
| Journal | J Mol Biol |
| Year | 1996 |
| Volume | 264 |
| Pages | 337-49 |
| Authors | Stahlberg J, Divne C, Koivula A, Piens K, Claeyssens M, Teeri TT, Jones TA |
| Title | Activity studies and crystal structures of catalytically deficient mutants of cellobiohydrolase I from Trichoderma reesei. |
| Related PDB | 2cel 3cel 4cel |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9449766 |
| Journal | Carbohydr Res |
| Year | 1997 |
| Volume | 304 |
| Pages | 143-54 |
| Authors | Klarskov K, Piens K, Stahlberg J, Hoj PB, Beeumen JV, Claeyssens M |
| Title | Cellobiohydrolase I from Trichoderma reesei: identification of an active-site nucleophile and additional information on sequence including the glycosylation pattern of the core protein. |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9325098 |
| Journal | J Mol Biol |
| Year | 1997 |
| Volume | 272 |
| Pages | 383-97 |
| Authors | Kleywegt GJ, Zou JY, Divne C, Davies GJ, Sinning I, Stahlberg J, Reinikainen T, Srisodsuk M, Teeri TT, Jones TA |
| Title |
The crystal structure of the catalytic core domain of endoglucanase I from Trichoderma reesei at 3.6 A resolution, |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | STRUCTURE BY NMR OF 478-513. |
| Medline ID | 97194052 |
| PubMed ID | 9041630 |
| Journal | Protein Sci |
| Year | 1997 |
| Volume | 6 |
| Pages | 294-303 |
| Authors | Mattinen ML, Kontteli M, Kerovuo J, Linder M, Annila A, Lindeberg G, Reinikainen T, Drakenberg T |
| Title | Three-dimensional structures of three engineered cellulose-binding domains of cellobiohydrolase I from Trichoderma reesei. |
| Related PDB | 1az6 1azh 1azj 1azk |
| Related UniProtKB | P62694 |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9761741 |
| Journal | Biochem J |
| Year | 1998 |
| Volume | 335 |
| Pages | 409-16 |
| Authors | MacKenzie LF, Sulzenbacher G, Divne C, Jones TA, Woldike HF, Schulein M, Withers SG, Davies GJ |
| Title | Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9760165 |
| Journal | Eur J Biochem |
| Year | 1998 |
| Volume | 256 |
| Pages | 279-86 |
| Authors | Mattinen ML, Linder M, Drakenberg T, Annila A |
| Title | Solution structure of the cellulose-binding domain of endoglucanase I from Trichoderma reesei and its interaction with cello-oligosaccharides. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 18-452. |
| Medline ID | 98128795 |
| PubMed ID | 9466911 |
| Journal | J Mol Biol |
| Year | 1998 |
| Volume | 275 |
| Pages | 309-25 |
| Authors | Divne C, Stahlberg J, Teeri TT, Jones TA |
| Title | High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei. |
| Related PDB | 5cel 6cel 7cel |
| Related UniProtKB | P62694 |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9989594 |
| Journal | FEBS Lett |
| Year | 1999 |
| Volume | 443 |
| Pages | 149-53 |
| Authors | Harjunpaa V, Helin J, Koivula A, Siika-aho M, Drakenberg T |
| Title |
A comparative study of two retaining enzymes of Trichoderma reesei: transglycosylation of oligosaccharides catalysed by the cellobiohydrolase I, |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11336632 |
| Journal | Biochem J |
| Year | 2001 |
| Volume | 356 |
| Pages | 19-30 |
| Authors | Becker D, Braet C, Brumer H 3rd, Claeyssens M, Divne C, Fagerstrom BR, Harris M, Jones TA, Kleywegt GJ, Koivula A, Mahdi S, Piens K, Sinnott ML, Stahlberg J, Teeri TT, Underwood M, Wohlfahrt G |
| Title | Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant. |
| Related PDB | 1egn |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11743726 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 314 |
| Pages | 1097-111 |
| Authors | Munoz IG, Ubhayasekera W, Henriksson H, Szabo I, Pettersson G, Johansson G, Mowbray SL, Stahlberg J |
| Title | Family 7 cellobiohydrolases from Phanerochaete chrysosporium: crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 A resolution and homology models of the isozymes. |
| Related PDB | 1gpi |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11114249 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 305 |
| Pages | 79-93 |
| Authors | Stahlberg J, Henriksson H, Divne C, Isaksson R, Pettersson G, Johansson G, Jones TA |
| Title | Structural basis for enantiomer binding and separation of a common beta-blocker: crystal structure of cellobiohydrolase Cel7A with bound (S)-propranolol at 1.9 A resolution. |
| Related PDB | 1dy4 |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12832787 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2003 |
| Volume | 59 |
| Pages | 1283-4 |
| Authors | Grassick A, Birrane G, Tuohy M, Murray P, Higgins T |
| Title | Crystallization and preliminary crystallographic analysis of the catalytic domain cellobiohydrolase I from Talaromyces emersonii. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12657782 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2003 |
| Volume | 59 |
| Pages | 637-43 |
| Authors | Munoz IG, Mowbray SL, Stahlberg J |
| Title | The catalytic module of Cel7D from Phanerochaete chrysosporium as a chiral selector: structural studies of its complex with the beta blocker (R)-propranolol. |
| Related PDB | 1h46 |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 14501131 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2003 |
| Volume | 59 |
| Pages | 1838-9 |
| Authors | Yaoi K, Kondo H, Suzuki M, Noro N, Tsuda S, Mitsuishi Y |
| Title |
Crystallization and preliminary X-ray crystallographic study on a xyloglucan-specific exo-beta-glycosidase, |
| Related PDB | |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12603317 |
| Journal | Eur J Biochem |
| Year | 2003 |
| Volume | 270 |
| Pages | 841-8 |
| Authors | Boer H, Koivula A |
| Title | The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A. |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 14568538 |
| Journal | J Mol Biol |
| Year | 2003 |
| Volume | 333 |
| Pages | 817-29 |
| Authors | von Ossowski I, Stahlberg J, Koivula A, Piens K, Becker D, Boer H, Harle R, Harris M, Divne C, Mahdi S, Zhao Y, Driguez H, Claeyssens M, Sinnott ML, Teeri TT |
| Title |
Engineering the exo-loop of Trichoderma reesei cellobiohydrolase, |
| Related PDB | 1q2b 1q2e |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15560790 |
| Journal | Eur J Biochem |
| Year | 2004 |
| Volume | 271 |
| Pages | 4495-506 |
| Authors | Grassick A, Murray PG, Thompson R, Collins CM, Byrnes L, Birrane G, Higgins TM, Tuohy MG |
| Title |
Three-dimensional structure of a thermostable native cellobiohydrolase, |
| Related PDB | 1q9h |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15819888 |
| Journal | FEBS J |
| Year | 2005 |
| Volume | 272 |
| Pages | 1952-64 |
| Authors | Ubhayasekera W, Munoz IG, Vasella A, Stahlberg J, Mowbray SL |
| Title | Structures of Phanerochaete chrysosporium Cel7D in complex with product andinhibitors. |
| Related PDB | 1z3t 1z3v 1z3w |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is composed of the N-terminal catalytic domain and the C-terminal small domain, This enzyme has got a similar catalytic site to that of alpha-amylase, Two of the acidic residues act as a nucleophile and an acid-base, |
| Created | Updated |
|---|---|
| 2004-01-21 | 2009-09-29 |