DB code: D00219
| RLCP classification | 1.13.30000.9 : Hydrolysis | |
|---|---|---|
| CATH domain | 3.30.70.80 : Alpha-Beta Plaits | |
| 3.40.50.200 : Rossmann fold | Catalytic domain | |
| E.C. | 3.4.21.62 | |
| CSA | 1sca | |
| M-CSA | 1sca | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.200 : Rossmann fold | S00295 S00296 S00519 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | MEROPS | Pfam | RefSeq |
|---|---|---|---|---|---|
| P00780 |
Subtilisin Carlsberg
|
EC
3.4.21.62
|
I09.001
()
|
PF05922
(Inhibitor_I9)
PF00082 (Peptidase_S8) [Graphical View] |
|
| P00782 |
Subtilisin BPN''
|
EC
3.4.21.62
Subtilisin Novo Subtilisin DFE Alkaline protease |
I09.001
()
|
PF05922
(Inhibitor_I9)
PF00082 (Peptidase_S8) [Graphical View] |
|
| P04189 |
Subtilisin E
|
EC
3.4.21.62
|
I09.001
()
|
PF05922
(Inhibitor_I9)
PF00082 (Peptidase_S8) [Graphical View] |
NP_388911.2
(Protein)
NC_000964.3 (DNA/RNA sequence) |
| P07518 |
Subtilisin
|
EC
3.4.21.62
Alkaline mesentericopeptidase |
S08.002
(Serine)
|
PF00082
(Peptidase_S8)
[Graphical View] |
| KEGG enzyme name |
|---|
|
subtilisin
alcalase alcalase 0.6L alcalase 2.5L ALK-enzyme bacillopeptidase A bacillopeptidase B Bacillus subtilis alkaline proteinase bioprase bioprase AL 15 bioprase APL 30 colistinase (see also comments) subtilisin J subtilisin S41 subtilisin Sendai subtilisin GX subtilisin E subtilisin BL genenase I esperase maxatase alcalase thermoase PC 10 protease XXVII thermoase superase subtilisin DY subtilopeptidase SP 266 savinase 8.0L savinase 4.0T kazusase protease VIII opticlean Bacillus subtilis alkaline proteinase protin A 3L savinase savinase 16.0L savinase 32.0 L EX orientase 10B protease S |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P00780 | SUBT_BACLI | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. | Secreted. | Binds 2 calcium ions per subunit. | |
| P00782 | SUBT_BACAM | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. | Monomer. | Secreted. | Binds 2 calcium ions per subunit. |
| P04189 | SUBT_BACSU | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. | Secreted. | Binds 2 calcium ions per subunit. | |
| P07518 | SUBT_BACPU | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. | Secreted. | Binds 2 calcium ions per subunit. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | |||||||||||
| KEGG-id | C00076 | C00012 | C00017 | C00001 | C00012 | C00017 | I00087 | I00085 | I00086 | |||||
| E.C. | ||||||||||||||
| Compound | Calcium | Peptide | Protein | H2O | Peptide | Protein | Peptidyl-tetrahedral intermediate | Acyl-enzyme | Tetrahedral intermediate | |||||
| Type | divalent metal (Ca2+, Mg2+) | peptide/protein | peptide/protein | H2O | peptide/protein | peptide/protein | ||||||||
| ChEBI |
29108 29108 |
15377 15377 |
||||||||||||
| PubChem |
271 271 |
22247451 962 22247451 962 |
||||||||||||
| 1scjB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1spbP |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1af4A |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1a2qA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ak9A |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1aqnA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1au9A |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1av7A |
|
|
|
|
|
Analogue:2x_NA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1avtA |
|
|
|
|
|
Analogue:2x_NA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1be6A |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:TCA | Unbound | |
| 1be8A |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:TCA | Unbound | |
| 1bfkA |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1bfuA |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1c3lA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Analogue:FMT_285 | Unbound | Unbound | Unbound | Unbound | |
| 1cseE |
|
|
|
|
|
Bound:2x_CA | Unbound | Bound:LEU_45-ASP_46 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1duiA |
|
|
|
|
|
Analogue:_NA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:DFP | |
| 1gnvA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1lw6E |
|
|
|
|
|
Bound:_CA | Unbound | Bound:MET_59-GLU_60 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1meeA |
|
|
|
|
|
Bound:2x_CA | Unbound | Bound:LYS_45-ASP_46 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1oyvA |
|
|
|
|
|
Bound:_CA | Unbound | Bound:PHE_62-ASN_63 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1oyvB |
|
|
|
|
|
Bound:_CA | Unbound | Bound:ARG_5-GLU_6 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s01A |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1s02A |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sbcA |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sbhA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sbiA |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sbnE |
|
|
|
|
|
Bound:2x_CA | Unbound | Bound:ARG_45-ASP_46 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sbtA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1scaA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1scbA |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1scdA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1scnE |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Intermediate-bound:BOC-ALA-PRO-APE (chain I) | Unbound | |
| 1scjA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Bound:TYR_377 (chain B) | Unbound | Unbound | Unbound | |
| 1selA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1selB |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sibE |
|
|
|
|
|
Bound:2x_CA | Unbound | Bound:LEU_45-ASP_46 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1spbS |
|
|
|
|
|
Analogue:_NA | Unbound | Unbound | Unbound | Bound:TYR_77 (chain P) | Unbound | Unbound | Unbound | |
| 1st2A |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1suaA |
|
|
|
|
|
Unbound | Unbound | Unbound | Bound:ALA-LEU-ALA-LEU (chain C) | Unbound | Unbound | Unbound | Unbound | |
| 1subA |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sucA |
|
|
|
|
|
Analogue:__K | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sudA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1sueA |
|
|
|
|
|
Analogue:_NA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:DFP | |
| 1supA |
|
|
|
|
|
Bound:_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Transition-state-analogue:PMS | |
| 1ubnA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1vsbA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1yjaA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1yjbA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1yjcA |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sbtA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2secE |
|
|
|
|
|
Bound:3x_CA | Unbound | Bound:LEU_59-ASP_60 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sicE |
|
|
|
|
|
Bound:2x_CA | Unbound | Bound:MET_73-VAL_74 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2sniE |
|
|
|
|
|
Bound:2x_CA | Unbound | Bound:MET_59-GLU_60 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 2st1A |
|
|
|
|
|
Bound:2x_CA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3sicE |
|
|
|
|
|
Bound:2x_CA | Unbound | Bound:LYS_73-VAL_74 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 3vsbA |
|
|
|
|
|
Analogue:2x_NA | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 5sicE |
|
|
|
|
|
Bound:2x_CA | Unbound | Bound:LYS_73-VAL_74 (chain I) | Unbound | Unbound | Unbound | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P00780, P00782, P04189, P07518 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1scjB |
|
|
|
|
|
|||||
| 1spbP |
|
|
|
|
|
|||||
| 1af4A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1a2qA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | MIS 221 | MIS 221 | mutant T22C, S87C, G169A, N218S | |
| 1ak9A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant T22C, M50F, S87C, G169A, Y217K, N218S | ||
| 1aqnA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant T22C, M50F, S87C, G169A, Q206C, Y217K, N218S | ||
| 1au9A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant T22C, M50F, S87C, G169A, Q206C, Y217K, N218S | ||
| 1av7A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | SBL 221 | SBL 221 | ||
| 1avtA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | CLD 221 | CLD 221 | ||
| 1be6A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1be8A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1bfkA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1bfuA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1c3lA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1cseE |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1duiA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1gnvA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | MIS 221 | MIS 221 | ||
| 1lw6E |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1meeA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1oyvA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1oyvB |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1s01A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant M50F, N76D, G169A, Q206C, Y217K, N218S | ||
| 1s02A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant Q19E, Q271E | ||
| 1sbcA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1sbhA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant M50F, N76D, G169A, Q206C, N218S, K256Y | ||
| 1sbiA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant M50F, N76D, G169A, Q206C, N218S | ||
| 1sbnE |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1sbtA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1scaA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1scbA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1scdA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1scnE |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1scjA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | mutant S221C | |||
| 1selA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | SEC 221 | SEC 221 | ||
| 1selB |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | SEC 221 | SEC 221 | ||
| 1sibE |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1spbS |
|
|
|
|
|
;HIS 64;ASN 155;THR 220; | mutant D32N, K43N, M50F, A73L, Q206V, Y217K, N218S, S221A, deletion 75-83 | |||
| 1st2A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1suaA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | mutant K43N, M50F, G73L, Q206V, Y217K, N218S, S221A, Q271E, deletion 75-83 | |||
| 1subA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | mutant N218S, S221C | |||
| 1sucA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | mutant M50P, Y217K, N218N, S221C, deletion 75-83 | |||
| 1sudA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | mutant M50P, Y217K, N218S, S221C | |||
| 1sueA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant Q2K, S3C, P5S, K43N, M50F, A73L, Q206C, Y217K, N218S, deletion 75-83 | ||
| 1supA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 1ubnA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | SOC 221 | SOC 221 | ||
| 1vsbA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | CLB 221 | CLB 221 | ||
| 1yjaA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant M50F, N76D, G169A, Q206C, N218S, K256Y | ||
| 1yjbA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant M50F, N76D, G169A, Q206C, N218S, K256Y | ||
| 1yjcA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | mutant M50F, N76D, G169A, Q206C, N218S, K256Y | ||
| 2sbtA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 2secE |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 2sicE |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 2sniE |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 2st1A |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 3sicE |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| 3vsbA |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220; | SBD 221 | SBD 221 | ||
| 5sicE |
|
|
|
|
|
ASP 32;HIS 64;ASN 155;THR 220;SER 221 | SER 221 | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[2]
|
||
|
[5]
|
||
|
[8]
|
p.397-399 | |
|
[9]
|
Fig.1 | |
|
[10]
|
p.6595 | |
|
[11]
|
p.7906 | |
|
[12]
|
p.275 | |
|
[16]
|
p.723 | |
|
[17]
|
Fig.1 | |
|
[18]
|
p.1219-1220 | |
|
[19]
|
Fig.1, p.11800 | |
|
[20]
|
Fig.2 | |
|
[22]
|
p.314-316 | |
|
[25]
|
Fig.4 | |
|
[55]
|
Fig.5 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | ACTIVE SITE. |
| Medline ID | 69104413 |
| PubMed ID | 5249818 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1968 |
| Volume | 61 |
| Pages | 1440-7 |
| Authors | Markland FS, Shaw E, Smith EL |
| Title | Identification of histidine 64 in the active site of subtilisin. |
| Related PDB | |
| Related UniProtKB | P00782 |
| [2] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). |
| Medline ID | 72035041 |
| PubMed ID | 4399039 |
| Journal | Philos Trans R Soc Lond B Biol Sci |
| Year | 1970 |
| Volume | 257 |
| Pages | 119-24 |
| Authors | Alden RA, Wright CS, Kraut J |
| Title | A hydrogen-bond network at the active site of subtilisin BPN'. |
| Related PDB | |
| Related UniProtKB | P00782 |
| [3] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 5160720 |
| Journal | Biochem Biophys Res Commun |
| Year | 1971 |
| Volume | 45 |
| Pages | 337-44 |
| Authors | Alden RA, Birktoft JJ, Kraut J, Robertus JD, Wright CS |
| Title | Atomic coordinates for subtilisin BPN' (or Novo). |
| Related PDB | 1sbt |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 4508127 |
| Journal | Cold Spring Harb Symp Quant Biol |
| Year | 1972 |
| Volume | 36 |
| Pages | 107-16 |
| Authors | Drenth J, Hol WG, Jansonius JN, Koekoek R |
| Title | A comparison of the three-dimensional structures of subtilisin BPN' and subtilisin novo. |
| Related PDB | 2sbt |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF COMPLEX WITH INHIBITOR. |
| Medline ID | 85033707 |
| PubMed ID | 6387152 |
| Journal | J Mol Biol |
| Year | 1984 |
| Volume | 178 |
| Pages | 389-414 |
| Authors | Hirono S, Akagawa H, Mitsui Y, Iitaka Y |
| Title | Crystal structure at 2.6 A resolution of the complex of subtilisin BPN' with streptomyces subtilisin inhibitor. |
| Related PDB | |
| Related UniProtKB | P00782 |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3519213 |
| Journal | EMBO J |
| Year | 1986 |
| Volume | 5 |
| Pages | 813-8 |
| Authors | Bode W, Papamokos E, Musil D, Seemueller U, Fritz H |
| Title |
Refined 1.2 A crystal structure of the complex formed between subtilisin Carlsberg and the inhibitor eglin c. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 3301348 |
| Journal | Eur J Biochem |
| Year | 1987 |
| Volume | 166 |
| Pages | 673-92 |
| Authors | Bode W, Papamokos E, Musil D |
| Title |
The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, |
| Related PDB | 1cse |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3299704 |
| Journal | Science |
| Year | 1987 |
| Volume | 237 |
| Pages | 394-9 |
| Authors | Carter P, Wells JA |
| Title | Engineering enzyme specificity by "substrate-assisted catalysis". |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3282170 |
| Journal | Nature |
| Year | 1988 |
| Volume | 332 |
| Pages | 564-8 |
| Authors | Carter P, Wells JA |
| Title | Dissecting the catalytic triad of a serine protease. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 3064813 |
| Journal | Biochemistry |
| Year | 1988 |
| Volume | 27 |
| Pages | 6582-98 |
| Authors | McPhalen CA, James MN |
| Title | Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo. |
| Related PDB | 2sec 2sni |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 3286644 |
| Journal | J Biol Chem |
| Year | 1988 |
| Volume | 263 |
| Pages | 7895-906 |
| Authors | Bott R, Ultsch M, Kossiakoff A, Graycar T, Katz B, Power S |
| Title | The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation. |
| Related PDB | 1st2 2st1 |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 3150541 |
| Journal | Protein Eng |
| Year | 1988 |
| Volume | 2 |
| Pages | 271-6 |
| Authors | Neidhart DJ, Petsko GA |
| Title | The refined crystal structure of subtilisin Carlsberg at 2.5 A resolution. |
| Related PDB | 1sbc |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANT. |
| Medline ID | 90057412 |
| PubMed ID | 2684274 |
| Journal | Biochemistry |
| Year | 1989 |
| Volume | 28 |
| Pages | 7205-13 |
| Authors | Pantoliano MW, Whitlow M, Wood JF, Dodd SW, Hardman KD, Rollence ML, Bryan PN |
| Title | Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding. |
| Related PDB | 1s01 1a2q 1ak9 1au9 |
| Related UniProtKB | P00782 |
| [14] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 2127106 |
| Journal | Protein Eng |
| Year | 1990 |
| Volume | 4 |
| Pages | 87-97 |
| Authors | Erwin CR, Barnett BL, Oliver JD, Sullivan JF |
| Title | Effects of engineered salt bridges on the stability of subtilisin BPN'. |
| Related PDB | 1s02 |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2199972 |
| Journal | Proteins |
| Year | 1990 |
| Volume | 7 |
| Pages | 343-57 |
| Authors | Katz B, Kossiakoff AA |
| Title | Crystal structures of subtilisin BPN' variants containing disulfide bonds and cavities: concerted structural rearrangements induced by mutagenesis. |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). |
| Medline ID | 92172311 |
| PubMed ID | 1793542 |
| Journal | Acta Crystallogr B |
| Year | 1991 |
| Volume | 47 |
| Pages | 707-30 |
| Authors | Dauter Z, Betzel C, Genov N, Pipon N, Wilson KS |
| Title | Complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-C. |
| Related PDB | 1mee |
| Related UniProtKB | P07518 |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2059622 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 6142-8 |
| Authors | Carter P, Abrahmsen L, Wells JA |
| Title | Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1991100 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 1211-21 |
| Authors | Kossiakoff AA, Ultsch M, White S, Eigenbrot C |
| Title | Neutron structure of subtilisin BPN': effects of chemical environment on hydrogen-bonding geometries and the pattern of hydrogen-deuterium exchange in secondary structure elements. |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1904870 |
| Journal | J Biol Chem |
| Year | 1991 |
| Volume | 266 |
| Pages | 11797-800 |
| Authors | Braxton S, Wells JA |
| Title | The importance of a distal hydrogen bonding group in stabilizing the transition state in subtilisin BPN'. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1904871 |
| Journal | J Biol Chem |
| Year | 1991 |
| Volume | 266 |
| Pages | 11801-9 |
| Authors | Mizushima N, Spellmeyer D, Hirono S, Pearlman D, Kollman P |
| Title | Free energy perturbation calculations on binding and catalysis after mutating threonine 220 in subtilisin. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 1992167 |
| Journal | J Mol Biol |
| Year | 1991 |
| Volume | 217 |
| Pages | 353-71 |
| Authors | Heinz DW, Priestle JP, Rahuel J, Wilson KS, Grutter MG |
| Title |
Refined crystal structures of subtilisin novo in complex with wild-type and two mutant eglins. |
| Related PDB | 1sbn 1sib |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 1920411 |
| Journal | J Mol Biol |
| Year | 1991 |
| Volume | 221 |
| Pages | 309-25 |
| Authors | Takeuchi Y, Satow Y, Nakamura KT, Mitsui Y |
| Title | Refined crystal structure of the complex of subtilisin BPN' and Streptomyces subtilisin inhibitor at 1.8 A resolution. |
| Related PDB | 2sic |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 1891457 |
| Journal | Protein Eng |
| Year | 1991 |
| Volume | 4 |
| Pages | 501-8 |
| Authors | Takeuchi Y, Noguchi S, Satow Y, Kojima S, Kumagai I, Miura K, Nakamura KT, Mitsui Y |
| Title | Molecular recognition at the active site of subtilisin BPN': crystallographic studies using genetically engineered proteinaceous inhibitor SSI (Streptomyces subtilisin inhibitor). |
| Related PDB | 3sic 5sic |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1453465 |
| Journal | J Mol Biol |
| Year | 1992 |
| Volume | 228 |
| Pages | 580-95 |
| Authors | Goddette DW, Paech C, Yang SS, Mielenz JR, Bystroff C, Wilke ME, Fletterick RJ |
| Title |
The crystal structure of the Bacillus lentus alkaline protease, |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8268166 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 13909-16 |
| Authors | Jackson SE, Fersht AR |
| Title | Contribution of long-range electrostatic interactions to the stabilization of the catalytic transition state of the serine protease subtilisin BPN'. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8347611 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 8112-9 |
| Authors | Strausberg S, Alexander P, Wang L, Schwarz F, Bryan P |
| Title | Catalysis of a protein folding reaction: thermodynamic and kinetic analysis of subtilisin BPN' interactions with its propeptide fragment. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF MUTANT WITH SELENOCYSTEINE-325. |
| Medline ID | 93291170 |
| PubMed ID | 8512925 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 6157-64 |
| Authors | Syed R, Wu ZP, Hogle JM, Hilvert D |
| Title | Crystal structure of selenosubtilisin at 2.0-A resolution. |
| Related PDB | 1sel |
| Related UniProtKB | P00780 |
| [28] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8378343 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1993 |
| Volume | 90 |
| Pages | 8653-7 |
| Authors | Fitzpatrick PA, Steinmetz AC, Ringe D, Klibanov AM |
| Title | Enzyme crystal structure in a neat organic solvent. |
| Related PDB | 1sca 1scb |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8332597 |
| Journal | Protein Eng |
| Year | 1993 |
| Volume | 6 |
| Pages | 397-408 |
| Authors | Heiner AP, Berendsen HJ, van Gunsteren WF |
| Title | Structure prediction of subtilisin BPN' mutants using molecular dynamics methods. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8332608 |
| Journal | Proteins |
| Year | 1993 |
| Volume | 16 |
| Pages | 205-13 |
| Authors | Gallagher T, Bryan P, Gilliland GL |
| Title | Calcium-independent subtilisin by design. |
| Related PDB | 1sub 1suc 1sud |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8297378 |
| Journal | Biochem Biophys Res Commun |
| Year | 1994 |
| Volume | 198 |
| Pages | 675-81 |
| Authors | Fitzpatrick PA, Ringe D, Klibanov AM |
| Title |
X-ray crystal structure of cross-linked subtilisin Carlsberg in water vs. |
| Related PDB | 1scd |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8068694 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 10535-44 |
| Authors | Steinmetz AC, Demuth HU, Ringe D |
| Title | Inactivation of subtilisin Carlsberg by N-((tert-butoxycarbonyl)alanylprolylphenylalanyl)-O-benzoylhydroxyl- amine: formation of a covalent enzyme-inhibitor linkage in the form of a carbamate derivative. |
| Related PDB | 1scn |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7547973 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 12302-10 |
| Authors | Wangikar PP, Rich JO, Clark DS, Dordick JS |
| Title | Probing enzymic transition state hydrophobicities. |
| Related PDB | |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8535784 |
| Journal | Structure |
| Year | 1995 |
| Volume | 3 |
| Pages | 907-14 |
| Authors | Gallagher T, Gilliland G, Wang L, Bryan P |
| Title | The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'. |
| Related PDB | 1spb |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) |
| Medline ID | |
| PubMed ID | |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1996 |
| Volume | 52 |
| Pages | 1125-35 |
| Authors | Gallagher T, Oliver J, Bott R, Betzel C, Gilliland GL |
| Title | Subtilisin BPN' at 1.6-A resolution: analysis for discrete disorder and comparison of crystal forms. |
| Related PDB | |
| Related UniProtKB | P00782 |
| [36] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8796321 |
| Journal | Adv Exp Med Biol |
| Year | 1996 |
| Volume | 379 |
| Pages | 159-69 |
| Authors | Gilliland GL, Gallagher DT, Alexander P, Bryan P |
| Title | Crystal structure analysis of subtilisin BPN' mutants engineered for studying thermal stability. |
| Related PDB | |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8796307 |
| Journal | Adv Exp Med Biol |
| Year | 1996 |
| Volume | 379 |
| Pages | 21-7 |
| Authors | Nonaka T, Suzuki T, Tanaka N, Saito S, Senda T, Miura K, Mitsui Y |
| Title | Structure and function of subtilisin BPN' as studied through crystallographic studies on a series of its complexes with genetically engineered proteinaceous inhibitor SSI. |
| Related PDB | |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | |
| Journal | J Am Chem Soc |
| Year | 1996 |
| Volume | 118 |
| Pages | 1645-50 |
| Authors | Kidd RD, Yennawar HP, Sears P, Wong CH, Farber GK |
| Title | A Weak Calcium Binding Site in Subtilisin BPN' Has a Dramatic Effect on Protein Stability. |
| Related PDB | 1sbi |
| Related UniProtKB | |
| [39] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9113975 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1997 |
| Volume | 94 |
| Pages | 4250-5 |
| Authors | Schmitke JL, Stern LJ, Klibanov AM |
| Title | The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile. |
| Related PDB | 1af4 |
| Related UniProtKB | |
| [40] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9675126 |
| Journal | Biochem Biophys Res Commun |
| Year | 1998 |
| Volume | 248 |
| Pages | 273-7 |
| Authors | Schmitke JL, Stern LJ, Klibanov AM |
| Title | Organic solvent binding to crystalline subtilisin1 in mostly aqueous media and in the neat solvents. |
| Related PDB | 1bfk 1bfu |
| Related UniProtKB | |
| [41] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 106-379. |
| Medline ID | 98087517 |
| PubMed ID | 9425066 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 451-62 |
| Authors | Stoll VS, Eger BT, Hynes RC, Martichonok V, Jones JB, Pai EF |
| Title | Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes. |
| Related PDB | 1av7 1avt 1vsb |
| Related UniProtKB | P00780 |
| [42] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). |
| Medline ID | 99030466 |
| PubMed ID | 9811547 |
| Journal | J Mol Biol |
| Year | 1998 |
| Volume | 284 |
| Pages | 137-44 |
| Authors | Jain SC, Shinde U, Li Y, Inouye M, Berman HM |
| Title | The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution. |
| Related PDB | 1scj |
| Related UniProtKB | P04189 |
| [43] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | |
| Journal | Journal of Crystal Growth |
| Year | 1998 |
| Volume | 193 |
| Pages | 665-73 |
| Authors | Gallagher DT, Pan QW, Gilliland GL |
| Title | Mechanism of ionic strength dependence of crystal growth rates in a subtilisin variant. |
| Related PDB | 1sue |
| Related UniProtKB | |
| [44] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9789015 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1998 |
| Volume | 95 |
| Pages | 12918-23 |
| Authors | Schmitke JL, Stern LJ, Klibanov AM |
| Title | Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water. |
| Related PDB | 1be6 1be8 |
| Related UniProtKB | |
| [45] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9552156 |
| Journal | Proteins |
| Year | 1998 |
| Volume | 31 |
| Pages | 21-32 |
| Authors | Almog O, Gallagher T, Tordova M, Hoskins J, Bryan P, Gilliland GL |
| Title | Crystal structure of calcium-independent subtilisin BPN' with restored thermal stability folded without the prodomain. |
| Related PDB | 1sua |
| Related UniProtKB | |
| [46] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9443341 |
| Journal | Proteins |
| Year | 1998 |
| Volume | 30 |
| Pages | 61-73 |
| Authors | Prange T, Schiltz M, Pernot L, Colloc'h N, Longhi S, Bourguet W, Fourme R |
| Title | Exploring hydrophobic sites in proteins with xenon or krypton. |
| Related PDB | 1c3l |
| Related UniProtKB | |
| [47] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10350485 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 6659-67 |
| Authors | Dinakarpandian D, Shenoy BC, Hilvert D, McRee DE, McTigue M, Carey PR |
| Title | Electric fields in active sites: substrate switching from null to strong fields in thiol- and selenol-subtilisins. |
| Related PDB | 1ubn |
| Related UniProtKB | |
| [48] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10048334 |
| Journal | Protein Sci |
| Year | 1999 |
| Volume | 8 |
| Pages | 410-7 |
| Authors | Kidd RD, Sears P, Huang DH, Witte K, Wong CH, Farber GK |
| Title | Breaking the low barrier hydrogen bond in a serine protease. |
| Related PDB | 1yja 1yjb 1yjc |
| Related UniProtKB | |
| [49] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11524007 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 10634-9 |
| Authors | Alexander PA, Ruan B, Bryan PN |
| Title | Cation-dependent stability of subtilisin. |
| Related PDB | |
| Related UniProtKB | |
| [50] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11524008 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 10640-4 |
| Authors | Alexander PA, Ruan B, Strausberg SL, Bryan PN |
| Title | Stabilizing mutations and calcium-dependent stability of subtilisin. |
| Related PDB | |
| Related UniProtKB | |
| [51] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11523982 |
| Journal | Biochemistry |
| Year | 2001 |
| Volume | 40 |
| Pages | 10411-6 |
| Authors | Stratton JR, Pelton JG, Kirsch JF |
| Title | A novel engineered subtilisin BPN' lacking a low-barrier hydrogen bond in the catalytic triad. |
| Related PDB | |
| Related UniProtKB | |
| [52] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12011071 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 27553-8 |
| Authors | Almog O, Gallagher DT, Ladner JE, Strausberg S, Alexander P, Bryan P, Gilliland GL |
| Title |
Structural basis of thermostability. |
| Related PDB | 1gnv |
| Related UniProtKB | |
| [53] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11925221 |
| Journal | J Org Chem |
| Year | 2002 |
| Volume | 67 |
| Pages | 2144-51 |
| Authors | Ema T, Jittani M, Furuie K, Utaka M, Sakai T |
| Title | 5-[4-(1-Hydroxyethyl)phenyl]-10,15,20-triphenylporphyrin as a probe of the transition-state conformation in hydrolase-catalyzed enantioselective transesterifications. |
| Related PDB | |
| Related UniProtKB | |
| [54] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12142461 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 2002 |
| Volume | 99 |
| Pages | 10316-21 |
| Authors | Radisky ES, Koshland DE Jr |
| Title | A clogged gutter mechanism for protease inhibitors. |
| Related PDB | 1lw6 |
| Related UniProtKB | |
| [55] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12684499 |
| Journal | J Biol Chem |
| Year | 2003 |
| Volume | 278 |
| Pages | 24062-71 |
| Authors | Barrette-Ng IH, Ng KK, Cherney MM, Pearce G, Ryan CA, James MN |
| Title | Structural basis of inhibition revealed by a 1:2 complex of the two-headed tomato inhibitor-II and subtilisin Carlsberg. |
| Related PDB | 1oyv |
| Related UniProtKB | |
| [56] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12649438 |
| Journal | Protein Sci |
| Year | 2003 |
| Volume | 12 |
| Pages | 794-810 |
| Authors | Day RM, Thalhauser CJ, Sudmeier JL, Vincent MP, Torchilin EV, Sanford DG, Bachovchin CW, Bachovchin WW |
| Title |
Tautomerism, |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme belongs to the peptidase family-S8.
The calcium ions are not involved in catalysis. This enzyme has got a classic catalytic triad, |
| Created | Updated |
|---|---|
| 2004-11-12 | 2011-02-21 |