DB code: D00185

CATH domain 3.40.350.10 : Creatine Amidinohydrolase; Chain A, domain 1
3.90.230.10 : Creatine Amidinohydrolase Catalytic domain
E.C. 3.4.11.9
CSA 1a16
M-CSA 1a16
MACiE

CATH domain Related DB codes (homologues)
3.40.350.10 : Creatine Amidinohydrolase; Chain A, domain 1 D00244
3.90.230.10 : Creatine Amidinohydrolase D00244

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq MEROPS Pfam
P15034 Xaa-Pro aminopeptidase
EC 3.4.11.9
X-Pro aminopeptidase
Aminopeptidase P II
APP-II
Aminoacylproline aminopeptidase
NP_417384.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491109.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
M24.004 (Metallo)
PF05195 (AMP_N)
PF00557 (Peptidase_M24)
[Graphical View]

KEGG enzyme name
Xaa-Pro aminopeptidase
proline aminopeptidase
aminopeptidase P
aminoacylproline aminopeptidase
X-Pro aminopeptidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P15034 AMPP_ECOLI Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide. Homotetramer. Cytoplasm. Binds 2 manganese ions per subunit.

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00034 C00012 C00001 C00045 C02186
E.C.
Compound Manganese Peptide H2O Amino acid Prolyl-peptide
Type heavy metal peptide/protein H2O amino acids amide group,amine group,carboxyl group
ChEBI 18291
 35154
 18291
 35154
 		15377
 15377
PubChem 23930
 23930
 		22247451
 962
 22247451
 962
1a16A01 Unbound Unbound Unbound Unbound
1az9A01 Unbound Unbound Unbound Unbound
1jawA01 Unbound Unbound Unbound Unbound
1m35A01 Unbound Unbound Unbound Unbound
1m35B01 Unbound Unbound Unbound Unbound
1m35C01 Unbound Unbound Unbound Unbound
1m35D01 Unbound Unbound Unbound Unbound
1m35E01 Unbound Unbound Unbound Unbound
1m35F01 Unbound Unbound Unbound Unbound
1a16A02 Bound:2x_MN Unbound Unbound Bound:PRO-LEU (chain L)
1az9A02 Bound:2x_MN Unbound Unbound Unbound
1jawA02 Bound:2x_MN Unbound Unbound Unbound
1m35A02 Bound:2x_MN Unbound Unbound Unbound
1m35B02 Bound:2x_MN Unbound Unbound Unbound
1m35C02 Bound:2x_MN Unbound Unbound Unbound
1m35D02 Bound:2x_MN Unbound Unbound Unbound
1m35E02 Bound:2x_MN Unbound Unbound Unbound
1m35F02 Bound:2x_MN Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot & literature [5]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1a16A01
1az9A01
1jawA01
1m35A01
1m35B01
1m35C01
1m35D01
1m35E01
1m35F01
1a16A02 GLU 383 ASP 260(Manganese-2 binding);HIS 354;GLU 383(Manganese-1 binding);ASP 271;GLU 406(Manganese-1 & -2 binding)
1az9A02 GLU 383 ASP 260(Manganese-2 binding);HIS 354;GLU 383(Manganese-1 binding);ASP 271;GLU 406(Manganese-1 & -2 binding)
1jawA02 GLU 383 ASP 260(Manganese-2 binding);HIS 354;GLU 383(Manganese-1 binding);ASP 271;GLU 406(Manganese-1 & -2 binding)
1m35A02 GLU 383 ASP 260(Manganese-2 binding);HIS 354;GLU 383(Manganese-1 binding);ASP 271;GLU 406(Manganese-1 & -2 binding)
1m35B02 GLU 383 ASP 260(Manganese-2 binding);HIS 354;GLU 383(Manganese-1 binding);ASP 271;GLU 406(Manganese-1 & -2 binding)
1m35C02 GLU 383 ASP 260(Manganese-2 binding);HIS 354;GLU 383(Manganese-1 binding);ASP 271;GLU 406(Manganese-1 & -2 binding)
1m35D02 GLU 383 ASP 260(Manganese-2 binding);HIS 354;GLU 383(Manganese-1 binding);ASP 271;GLU 406(Manganese-1 & -2 binding)
1m35E02 GLU 383 ASP 260(Manganese-2 binding);HIS 354;GLU 383(Manganese-1 binding);ASP 271;GLU 406(Manganese-1 & -2 binding)
1m35F02 GLU 383 ASP 260(Manganese-2 binding);HIS 354;GLU 383(Manganese-1 binding);ASP 271;GLU 406(Manganese-1 & -2 binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[5]
Fig.3 2
[6]
Fig.7, p.14817-14818 3
[8]
p.15134-15135

References
[1]
Resource
Comments
Medline ID
PubMed ID 4318
Journal Eur J Biochem
Year 1976
Volume 63
Pages 271-87
Authors Kessler E, Yaron A
Title An extracellular aminopeptidase from Clostridium histolyticum.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 7397119
Journal Biochemistry
Year 1980
Volume 19
Pages 3055-9
Authors Lin LN, Brandts JF
Title Kinetic mechanism for conformational transitions between poly-L-prolines I and II: a study utilizing the cis-trans specificity of a proline-specific protease.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 6441592
Journal Biochemistry
Year 1984
Volume 23
Pages 5713-23
Authors Lin LN, Brandts JF
Title Involvement of prolines-114 and -117 in the slow refolding phase of ribonuclease A as determined by isomer-specific proteolysis.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 8146141
Journal Proc Natl Acad Sci U S A
Year 1994
Volume 91
Pages 2473-7
Authors Bazan JF, Weaver LH, Roderick SL, Huber R, Matthews BW
Title Sequence and structure comparison suggest that methionine aminopeptidase, prolidase, aminopeptidase P, and creatinase share a common fold.
Related PDB
Related UniProtKB
[5]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)
Medline ID 98188227
PubMed ID 9520390
Journal Proc Natl Acad Sci U S A
Year 1998
Volume 95
Pages 3472-7
Authors Wilce MC, Bond CS, Dixon NE, Freeman HC, Guss JM, Lilley PE, Wilce JA
Title Structure and mechanism of a proline-specific aminopeptidase from Escherichia coli.
Related PDB 1a16 1az9 1jaw
Related UniProtKB P15034
[6]
Resource
Comments
Medline ID
PubMed ID 10555963
Journal Biochemistry
Year 1999
Volume 38
Pages 14810-9
Authors Lowther WT, Zhang Y, Sampson PB, Honek JF, Matthews BW
Title Insights into the mechanism of Escherichia coli methionine aminopeptidase from the structural analysis of reaction products and phosphorus-based transition-state analogues.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 10395480
Journal J Med Chem
Year 1999
Volume 42
Pages 2394-402
Authors Maggiora LL, Orawski AT, Simmons WH
Title Apstatin analogue inhibitors of aminopeptidase P, a bradykinin-degrading enzyme.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 11106491
Journal Biochemistry
Year 2000
Volume 39
Pages 15129-35
Authors Cottrell GS, Hyde RJ, Lim J, Parsons MR, Hooper NM, Turner AJ
Title Identification of critical residues in the active site of porcine membrane-bound aminopeptidase P.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 11516173
Journal Arch Biochem Biophys
Year 2001
Volume 393
Pages 163-9
Authors Iwase A, Nomura S, Mizutani S
Title Characterization of a secretase activity for placental leucine aminopeptidase.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 11423553
Journal J Biol Chem
Year 2001
Volume 276
Pages 31732-7
Authors Hauser F, Strassner J, Schaller A
Title Cloning, expression, and characterization of tomato (Lycopersicon esculentum) aminopeptidase P.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 11872174
Journal J Biochem (Tokyo)
Year 2002
Volume 131
Pages 445-52
Authors Kulkarni GV, Deobagkar DD
Title A cytosolic form of aminopeptidase P from Drosophila melanogaster: molecular cloning and characterization.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-03-25 2009-02-26