DB code: D00127

CATH domain 1.10.135.10 : Transferase Creatine Kinase; Chain A, domain 1
3.30.590.10 : Creatine Kinase; Chain A, domain 2 Catalytic domain
E.C. 2.7.3.3
CSA 1bg0
M-CSA 1bg0
MACiE M0086

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
P51541 Arginine kinase
AK
EC 2.7.3.3
PF00217 (ATP-gua_Ptrans)
PF02807 (ATP-gua_PtransN)
[Graphical View]

KEGG enzyme name
arginine kinase
arginine phosphokinase
adenosine 5'-triphosphate: L-arginine phosphotransferase
adenosine 5'-triphosphate-arginine phosphotransferase
ATP:L-arginine N-phosphotransferasel ATP:L-arginineomega-N-phosphotransferase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P51541 KARG_LIMPO ATP + L-arginine = ADP + N(omega)-phospho-L- arginine. Monomer. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.
MAP00330 Arginine and proline metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00305 C00002 C00062 C00008 C05945
E.C.
Compound Magnesium ATP L-Arginine ADP N-Phospho-L-arginine
Type divalent metal (Ca2+, Mg2+) amine group,nucleotide amino acids,amine group,imine group,lipid amine group,nucleotide amino acids,amine group,imine group,lipid,phosphate group/phosphate ion
ChEBI 18420
 18420
 		15422
 15422
 		16467
 16467
 		16761
 16761
 		18412
 18412
PubChem 888
 888
 		5957
 5957
 		28782
 6322
 28782
 6322
 		6022
 6022
 		92150
 92150
1bg0A01 Unbound Unbound Unbound Unbound Unbound Unbound
1m80A01 Unbound Unbound Unbound Unbound Unbound Unbound
1m80B01 Unbound Unbound Unbound Unbound Unbound Unbound
1m15A01 Unbound Unbound Unbound Unbound Unbound Unbound
1p50A01 Unbound Unbound Unbound Unbound Unbound Unbound
1p52A01 Unbound Unbound Unbound Unbound Unbound Unbound
1bg0A02 Bound:_MG Unbound Analogue:DAR Bound:ADP Unbound Transition-state-analogue:NO3_401
1m80A02 Unbound Unbound Unbound Unbound Unbound Unbound
1m80B02 Unbound Unbound Unbound Unbound Unbound Unbound
1m15A02 Bound:_MG Unbound Bound:ARG Bound:ADP Unbound Transition-state-analogue:NO3_405
1p50A02 Bound:_MG Unbound Bound:ARG Bound:ADP Unbound Transition-state-analogue:NO3
1p52A02 Bound:_MG Unbound Analogue:DAR Bound:ADP Unbound Transition-state-analogue:NO3_401

Reference for Active-site residues
resource references E.C.
Swiss-prot;P51541

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1bg0A01
1m80A01
1m80B01
1m15A01
1p50A01
1p52A01
1bg0A02 CYS 271
1m80A02 CYS 271
1m80B02 CYS 271
1m15A02 CYS 271
1p50A02 CYS 271
1p52A02 CYS 271

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[4]
Fig.3, p.8451-8453
[10]
p.26956-26957

References
[1]
Resource
Comments
Medline ID
PubMed ID 7819288
Journal Biochim Biophys Acta
Year 1995
Volume 1246
Pages 197-200
Authors Strong SJ, Ellington WR
Title Isolation and sequence analysis of the gene for arginine kinase from the chelicerate arthropod, Limulus polyphemus: insights into catalytically important residues.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 9359868
Journal Biochem J
Year 1997
Volume 328
Pages 301-6
Authors Suzuki T, Kawasaki Y, Furukohri T
Title Evolution of phosphagen kinase. Isolation, characterization and cDNA-derived amino acid sequence of two-domain arginine kinase from the sea anemone Anthopleura japonicus.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 9675202
Journal Biophys J
Year 1998
Volume 75
Pages 1016-23
Authors Forstner M, Kriechbaum M, Laggner P, Wallimann T
Title Structural changes of creatine kinase upon substrate binding.
Related PDB
Related UniProtKB
[4]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS)
Medline ID 98337935
PubMed ID 9671698
Journal Proc Natl Acad Sci U S A
Year 1998
Volume 95
Pages 8449-54
Authors Zhou G, Somasundaram T, Blanc E, Parthasarathy G, Ellington WR, Chapman MS
Title Transition state structure of arginine kinase: implications for catalysis of bimolecular reactions.
Related PDB 1bg0
Related UniProtKB P51541
[5]
Resource
Comments
Medline ID
PubMed ID 10089314
Journal Acta Crystallogr D Biol Crystallogr
Year 1999
Volume 55
Pages 835-45
Authors Zhou G, Somasundaram T, Blanc E, Chen Z, Chapman MS
Title Critical initial real-space refinement in the structure determination of arginine kinase.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 10461938
Journal J Parasitol
Year 1999
Volume 85
Pages 603-7
Authors Platzer EG, Wang W, Thompson SN, Borchardt DB
Title Arginine kinase and phosphoarginine, a functional phosphagen, in the rhabditoid nematode steinernema carpocapsae.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 10811656
Journal J Biol Chem
Year 2000
Volume 275
Pages 23884-90
Authors Suzuki T, Fukuta H, Nagato H, Umekawa M
Title Arginine kinase from Nautilus pompilius, a living fossil. Site-directed mutagenesis studies on the role of amino acid residues in the Guanidino specificity region.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 12454458
Journal Acta Crystallogr D Biol Crystallogr
Year 2002
Volume 58
Pages 2009-17
Authors Yousef MS, Fabiola F, Gattis JL, Somasundaram T, Chapman MS
Title Refinement of the arginine kinase transition-state analogue complex at 1.2 A resolution: mechanistic insights.
Related PDB 1m15
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 12493833
Journal Protein Sci
Year 2003
Volume 12
Pages 103-11
Authors Yousef MS, Clark SA, Pruett PK, Somasundaram T, Ellington WR, Chapman MS
Title Induced fit in guanidino kinases--comparison of substrate-free and transition state analog structures of arginine kinase.
Related PDB 1m80
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 12732621
Journal J Biol Chem
Year 2003
Volume 278
Pages 26952-7
Authors Pruett PS, Azzi A, Clark SA, Yousef MS, Gattis JL, Somasundaram T, Ellington WR, Chapman MS
Title The putative catalytic bases have, at most, an accessory role in the mechanism of arginine kinase.
Related PDB 1p50 1p52
Related UniProtKB

Comments

Created Updated
2004-03-18 2009-02-26