DB code: D00083
| CATH domain | 1.10.155.10 : Chemotaxis Receptor Methyltransferase Cher; domain 1 | |
|---|---|---|
| 3.40.50.150 : Rossmann fold | Catalytic domain | |
| E.C. | 2.1.1.80 | |
| CSA | 1af7 | |
| M-CSA | 1af7 | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.150 : Rossmann fold | S00637 S00639 S00262 S00261 S00291 S00412 D00075 D00076 D00079 D00080 D00082 D00823 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P07801 |
Chemotaxis protein methyltransferase
|
EC
2.1.1.80
|
NP_460875.1
(Protein)
NC_003197.1 (DNA/RNA sequence) |
PF01739
(CheR)
PF03705 (CheR_N) [Graphical View] |
| KEGG enzyme name |
|---|
|
protein-glutamate O-methyltransferase
methyl-accepting chemotaxis protein O-methyltransferase S-adenosylmethionine-glutamyl methyltransferase methyl-accepting chemotaxis protein methyltransferase II S-adenosylmethionine:protein-carboxyl O-methyltransferase protein methylase II MCP methyltransferase I MCP methyltransferase II protein O-methyltransferase protein(aspartate)methyltransferase protein(carboxyl)methyltransferase protein carboxyl-methylase protein carboxyl-O-methyltransferase protein carboxylmethyltransferase II protein carboxymethylase protein carboxymethyltransferase protein methyltransferase II |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P07801 | CHER_SALTY | S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00019 | C00614 | C00021 | C04142 | ||||||
| E.C. | ||||||||||
| Compound | S-Adenosyl-L-methionine | Protein glutamate | S-Adenosyl-L-homocysteine | Protein glutamate methyl ester | ||||||
| Type | amino acids,amine group,nucleoside,sulfonium ion | carboxyl group,peptide/protein | amino acids,amine group,nucleoside,sulfide group | peptide/protein | ||||||
| ChEBI |
67040 67040 |
16680 57856 16680 57856 |
||||||||
| PubChem |
34755 34755 |
25246222 439155 25246222 439155 |
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| 1af7A01 |
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Unbound | Unbound | Unbound | Unbound | |
| 1bc5A01 |
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Unbound | Analogue:ACE-ASN-TRP-GLU-THR-PHE (chain T) | Unbound | Unbound | |
| 1af7A02 |
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Unbound | Unbound | Bound:SAH | Unbound | |
| 1bc5A02 |
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Unbound | Unbound | Bound:SAH | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| PDB;1bc5 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1af7A01 |
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| 1bc5A01 |
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| 1af7A02 |
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HIS 114;HIS 192(Cobalt binding) | ||||
| 1bc5A02 |
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HIS 114;HIS 192 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[2]
|
p.554-556 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7567955 |
| Journal | Proteins |
| Year | 1995 |
| Volume | 21 |
| Pages | 345-50 |
| Authors | West AH, Djordjevic S, Martinez-Hackert E, Stock AM |
| Title |
Purification, |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 11-284 |
| Medline ID | 97277239 |
| PubMed ID | 9115443 |
| Journal | Structure |
| Year | 1997 |
| Volume | 5 |
| Pages | 545-58 |
| Authors | Djordjevic S, Stock AM |
| Title | Crystal structure of the chemotaxis receptor methyltransferase CheR suggests a conserved structural motif for binding S-adenosylmethionine. |
| Related PDB | 1af7 |
| Related UniProtKB | P07801 |
| [3] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 16-284 |
| Medline ID | 98290446 |
| PubMed ID | 9628482 |
| Journal | Nat Struct Biol |
| Year | 1998 |
| Volume | 5 |
| Pages | 446-50 |
| Authors | Djordjevic S, Stock AM |
| Title | Chemotaxis receptor recognition by protein methyltransferase CheR. |
| Related PDB | 1bc5 |
| Related UniProtKB | P07801 |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9731767 |
| Journal | Nat Struct Biol |
| Year | 1998 |
| Volume | 5 |
| Pages | 763-4 |
| Authors | Yang F, Gustafson KR, Boyd MR, Wlodawer A |
| Title | Crystal structure of Escherichia coli HdeA. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9465023 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1998 |
| Volume | 95 |
| Pages | 1381-6 |
| Authors | Djordjevic S, Goudreau PN, Xu Q, Stock AM, West AH |
| Title | Structural basis for methylesterase CheB regulation by a phosphorylation-activated domain. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10760170 |
| Journal | Mol Microbiol |
| Year | 2000 |
| Volume | 36 |
| Pages | 132-40 |
| Authors | Shiomi D, Okumura H, Homma M, Kawagishi I |
| Title | The aspartate chemoreceptor Tar is effectively methylated by binding to the methyltransferase mainly through hydrophobic interaction. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11916840 |
| Journal | Biophys J |
| Year | 2002 |
| Volume | 82 |
| Pages | 1809-17 |
| Authors | Levin MD, Shimizu TS, Bray D |
| Title | Binding and diffusion of CheR molecules within a cluster of membrane receptors. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
| Created | Updated |
|---|---|
| 2004-03-24 | 2009-02-26 |