DB code: D00061

CATH domain 1.10.520.10 : Peroxidase; domain 1 Catalytic domain
1.10.420.10 : Peroxidase; domain 2 Catalytic domain
E.C. 1.11.1.13
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)
1.10.420.10 : Peroxidase; domain 2 D00060 D00062
1.10.520.10 : Peroxidase; domain 1 D00060 D00062

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
Q02567 Peroxidase manganese-dependent 1
EC 1.11.1.13
Peroxidase manganese-dependent I
Manganese peroxidase isozyme 1
MnP-1
MnP1
PF11895 (DUF3415)
PF00141 (peroxidase)
[Graphical View]

KEGG enzyme name
manganese peroxidase
peroxidase-M2
Mn-dependent (NADH-oxidizing) peroxidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
Q02567 PEM1_PHACH 2 Mn(2+) + 2 H(+) + H(2)O(2) = 2 Mn(3+) + 2 H(2)O. Secreted. Binds 2 calcium ions per subunit. Binds 1 heme B (iron-protoporphyrin IX) group per subunit.

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00032 C00076 C00034 C00080 C00027 C00034 C00001
E.C.
Compound Heme Calcium Mn(II) H+ H2O2 Mn(III) H2O
Type aromatic ring (with nitrogen atoms),carboxyl group,heavy metal divalent metal (Ca2+, Mg2+) heavy metal others others heavy metal H2O
ChEBI 17627
 26355
 17627
 26355
 		29108
 29108
 		18291
 35154
 18291
 35154
 		15378
 15378
 		16240
 16240
 		18291
 35154
 18291
 35154
 		15377
 15377
PubChem 271
 271
 		23930
 23930
 		1038
 1038
 		22326046
 784
 22326046
 784
 		23930
 23930
 		22247451
 962
 22247451
 962
1mn1A01 Unbound Bound:_CA Unbound Unbound Unbound
1mn2A01 Unbound Bound:_CA Unbound Unbound Unbound
1mnpA01 Unbound Bound:_CA Bound:_MN Unbound Unbound
1yydA01 Unbound Bound:_CA Bound:_MN Unbound Unbound
1yygA01 Unbound Bound:_CA_372 Analogue:_CA_381 Unbound Unbound
1yzpA01 Unbound Bound:_CA Unbound Unbound Unbound
1yzrA01 Unbound Bound:_CA Analogue:_SM Unbound Unbound
1mn1A02 Bound:HEM Bound:_CA Unbound Unbound Unbound
1mn2A02 Bound:HEM Bound:_CA Unbound Unbound Unbound
1mnpA02 Bound:HEM Bound:_CA Unbound Unbound Unbound
1yydA02 Bound:HEM Bound:_CA Unbound Unbound Unbound
1yygA02 Bound:HEM Bound:_CA_371 Unbound Unbound Unbound
1yzpA02 Bound:HEM Bound:_CA Unbound Unbound Unbound
1yzrA02 Bound:HEM Bound:_CA Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;Q02567 & literature [37]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1mn1A01 ARG 42;PHE 45;HIS 46;GLU 74;ASN 80 GLU 35;GLU 39(Manganese binding);ASP 47;GLY 62;ASP 64;SER 66(Calcium-1 binding)
1mn2A01 ARG 42;PHE 45;HIS 46;GLU 74;ASN 80 ;GLU 39(Manganese binding);ASP 47;GLY 62;ASP 64;SER 66(Calcium-1 binding) mutant E35Q
1mnpA01 ARG 42;PHE 45;HIS 46;GLU 74;ASN 80 GLU 35;GLU 39(Manganese binding);ASP 47;GLY 62;ASP 64;SER 66(Calcium-1 binding)
1yydA01 ARG 42;PHE 45;HIS 46;GLU 74;ASN 80 GLU 35;GLU 39(Manganese binding);ASP 47;GLY 62;ASP 64;SER 66(Calcium-1 binding)
1yygA01 ARG 42;PHE 45;HIS 46;GLU 74;ASN 80 GLU 35;GLU 39(Manganese binding);ASP 47;GLY 62;ASP 64;SER 66(Calcium-1 binding)
1yzpA01 ARG 42;PHE 45;HIS 46;GLU 74;ASN 80 GLU 35;GLU 39(Manganese binding);ASP 47;GLY 62;ASP 64;SER 66(Calcium-1 binding)
1yzrA01 ARG 42;PHE 45;HIS 46;GLU 74;ASN 80 GLU 35;GLU 39(Manganese binding);ASP 47;GLY 62;ASP 64;SER 66(Calcium-1 binding)
1mn1A02 HIS 173(Heme binding); ;SER 174;ASP 191;THR 193;THR 196;ASP 198(Calcium-2 binding) mutant D179N
1mn2A02 HIS 173(Heme binding); ;SER 174;ASP 191;THR 193;THR 196;ASP 198(Calcium-2 binding) mutant D179N
1mnpA02 HIS 173(Heme binding);ASP 179(Manganese binding);SER 174;ASP 191;THR 193;THR 196;ASP 198(Calcium-2 binding)
1yydA02 HIS 173(Heme binding);ASP 179(Manganese binding);SER 174;ASP 191;THR 193;THR 196;ASP 198(Calcium-2 binding)
1yygA02 HIS 173(Heme binding);ASP 179(Manganese binding);SER 174;ASP 191;THR 193;THR 196;ASP 198(Calcium-2 binding)
1yzpA02 HIS 173(Heme binding);ASP 179(Manganese binding);SER 174;ASP 191;THR 193;THR 196;ASP 198(Calcium-2 binding)
1yzrA02 HIS 173(Heme binding);ASP 179(Manganese binding);SER 174;ASP 191;THR 193;THR 196;ASP 198(Calcium-2 binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[7]
[10]
[19]
p.3659
[20]
[22]
SCHEME 1
[23]
[25]
[37]
Fig.2, p.563-565, Fig.5, Fig.6, p.571-574

References
[1]
Resource
Comments
Medline ID
PubMed ID 2925681
Journal J Biol Chem
Year 1989
Volume 264
Pages 5036-40
Authors Pribnow D, Mayfield MB, Nipper VJ, Brown JA, Gold MH
Title Characterization of a cDNA encoding a manganese peroxidase, from the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 1851156
Journal J Biol Chem
Year 1991
Volume 266
Pages 8751-8
Authors Harris RZ, Wariishi H, Gold MH, Ortiz de Montellano PR
Title The catalytic site of manganese peroxidase. Regiospecific addition of sodium azide and alkylhydrazines to the heme group.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 1327129
Journal Biochemistry
Year 1992
Volume 31
Pages 10009-17
Authors Banci L, Bertini I, Pease EA, Tien M, Turano P
Title 1H NMR investigation of manganese peroxidase from Phanerochaete chrysosporium. A comparison with other peroxidases.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 8393660
Journal Biochem J
Year 1993
Volume 293
Pages 431-5
Authors Ander P, Sena-Martins G, Duarte JC
Title Influence of cellobiose oxidase on peroxidases from Phanerochaete chrysosporium.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 7763834
Journal J Biotechnol
Year 1993
Volume 30
Pages 79-90
Authors Cai D, Tien M
Title Lignin-degrading peroxidases of Phanerochaete chrysosporium.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 8034057
Journal FEBS Lett
Year 1994
Volume 348
Pages 291-6
Authors Lundell T, Hatakka A
Title Participation of Mn(II) in the catalysis of laccase, manganese peroxidase and lignin peroxidase from Phelbia radiata.
Related PDB
Related UniProtKB
[7]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS)
Medline ID 95105154
PubMed ID 7806497
Journal J Biol Chem
Year 1994
Volume 269
Pages 32759-67
Authors Sundaramoorthy M, Kishi K, Gold MH, Poulos TL
Title The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-A resolution.
Related PDB
Related UniProtKB Q02567
[8]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 8182752
Journal J Mol Biol
Year 1994
Volume 238
Pages 845-8
Authors Sundaramoorthy M, Kishi K, Gold MH, Poulos TL
Title Preliminary crystallographic analysis of manganese peroxidase from Phanerochaete chrysosporium.
Related PDB 1mnp
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 7731950
Journal Proteins
Year 1994
Volume 20
Pages 312-9
Authors Johnson F, Loew GH, Du P
Title Homology models of two isozymes of manganese peroxidase: prediction of a Mn(II) binding site.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 7672248
Journal Biochem Soc Trans
Year 1995
Volume 23
Pages 228-32
Authors Poulos TL, Patterson WR, Sundaramoorthy M
Title The crystal structure of ascorbate and manganese peroxidases: the role of non-haem metal in the catalytic mechanism.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 7654716
Journal Biochemistry
Year 1995
Volume 34
Pages 10620-7
Authors Kusters-van Someren M, Kishi K, Lundell T, Gold MH
Title The manganese binding site of manganese peroxidase: characterization of an Asp179Asn site-directed mutant protein.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 7548080
Journal Biochemistry
Year 1995
Volume 34
Pages 13176-82
Authors Sinclair R, Copeland B, Yamazaki I, Powers L
Title X-ray absorption spectroscopy comparison of the active site structures of Phanerochaete chrysosporium lignin peroxidase isoenzymes H2, H3, H4, H5, H8, and H10.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 7737200
Journal Eur J Biochem
Year 1995
Volume 228
Pages 955-61
Authors Selvaggini C, Salmona M, De Gioia L
Title Manganese peroxidase from Phanerochaete chrysosporium. A homology-based molecular model.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 8806717
Journal Arch Biochem Biophys
Year 1996
Volume 332
Pages 128-34
Authors Sutherland GR, Aust SD
Title The effects of calcium on the thermal stability and activity of manganese peroxidase.
Related PDB
Related UniProtKB
[15]
Resource
Comments METAL-BINDING
Medline ID 96280658
PubMed ID 8688436
Journal Biochemistry
Year 1996
Volume 35
Pages 8986-94
Authors Kishi K, Kusters-van Someren M, Mayfield MB, Sun J, Loehr TM, Gold MH
Title Characterization of manganese(II) binding site mutants of manganese peroxidase.
Related PDB
Related UniProtKB Q02567
[16]
Resource
Comments
Medline ID
PubMed ID 8647081
Journal Eur J Biochem
Year 1996
Volume 237
Pages 424-32
Authors Martinez MJ, Ruiz-Duenas FJ, Guillen F, Martinez AT
Title Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii.
Related PDB
Related UniProtKB
[17]
Resource
Comments
Medline ID
PubMed ID 9100022
Journal Biochemistry
Year 1997
Volume 36
Pages 4268-77
Authors Kishi K, Hildebrand DP, Kusters-van Someren M, Gettemy J, Mauk AG, Gold MH
Title Site-directed mutations at phenylalanine-190 of manganese peroxidase: effects on stability, function, and coordination.
Related PDB
Related UniProtKB
[18]
Resource
Comments
Medline ID
PubMed ID 9214302
Journal Biochemistry
Year 1997
Volume 36
Pages 8567-73
Authors Sutherland GR, Aust SD
Title Thermodynamics of binding of the distal calcium to manganese peroxidase.
Related PDB
Related UniProtKB
[19]
Resource
Comments
Medline ID
PubMed ID 9132018
Journal Biochemistry
Year 1997
Volume 36
Pages 3654-62
Authors Sutherland GR, Zapanta LS, Tien M, Aust SD
Title Role of calcium in maintaining the heme environment of manganese peroxidase.
Related PDB
Related UniProtKB
[20]
Resource
Comments
Medline ID
PubMed ID 9245408
Journal Biochemistry
Year 1997
Volume 36
Pages 9766-73
Authors Whitwam RE, Brown KR, Musick M, Natan MJ, Tien M
Title Mutagenesis of the Mn2+-binding site of manganese peroxidase affects oxidation of Mn2+ by both compound I and compound II.
Related PDB
Related UniProtKB
[21]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF MUTANTS
Medline ID 97362247
PubMed ID 9211904
Journal J Biol Chem
Year 1997
Volume 272
Pages 17574-80
Authors Sundaramoorthy M, Kishi K, Gold MH, Poulos TL
Title Crystal structures of substrate binding site mutants of manganese peroxidase.
Related PDB 1mn1 1mn2
Related UniProtKB Q02567
[22]
Resource
Comments
Medline ID
PubMed ID 9705219
Journal Arch Biochem Biophys
Year 1998
Volume 356
Pages 287-95
Authors Timofeevski SL, Reading NS, Aust SD
Title Mechanisms for protection against inactivation of manganese peroxidase by hydrogen peroxide.
Related PDB
Related UniProtKB
[23]
Resource
Comments
Medline ID
PubMed ID 9636044
Journal Biochemistry
Year 1998
Volume 37
Pages 9009-15
Authors Banci L, Bertini I, Dal Pozzo L, Del Conte R, Tien M
Title Monitoring the role of oxalate in manganese peroxidase.
Related PDB
Related UniProtKB
[24]
Resource
Comments
Medline ID
PubMed ID 10099422
Journal Biotechnol Bioeng
Year 1998
Volume 60
Pages 204-15
Authors Grabski AC, Grimek HJ, Burgess RR
Title Immobilization of manganese peroxidase from Lentinula edodes and its biocatalytic generation of MnIII-chelate as a chemical oxidant of chlorophenols.
Related PDB
Related UniProtKB
[25]
Resource
Comments
Medline ID
PubMed ID 9742955
Journal FEBS Lett
Year 1998
Volume 434
Pages 362-6
Authors Hofrichter M, Ziegenhagen D, Vares T, Friedrich M, Jager MG, Fritsche W, Hatakka A
Title Oxidative decomposition of malonic acid as basis for the action of manganese peroxidase in the absence of hydrogen peroxide.
Related PDB
Related UniProtKB
[26]
Resource
Comments
Medline ID
PubMed ID 9469932
Journal Gene
Year 1998
Volume 206
Pages 185-93
Authors Lobos S, Larrondo L, Salas L, Karahanian E, Vicuna R
Title Cloning and molecular analysis of a cDNA and the Cs-mnp1 gene encoding a manganese peroxidase isoenzyme from the lignin-degrading basidiomycete Ceriporiopsis subvermispora.
Related PDB
Related UniProtKB
[27]
Resource
Comments
Medline ID
PubMed ID 9624124
Journal J Biol Chem
Year 1998
Volume 273
Pages 15412-7
Authors Mester T, Field JA
Title Characterization of a novel manganese peroxidase-lignin peroxidase hybrid isozyme produced by Bjerkandera species strain BOS55 in the absence of manganese.
Related PDB
Related UniProtKB
[28]
Resource
Comments
Medline ID
PubMed ID 10423239
Journal Biochemistry
Year 1999
Volume 38
Pages 9617-25
Authors Banci L, Bertini I, Capannoli C, Del Conte R, Tien M
Title Spectroscopic characterization of active mutants of manganese peroxidase: mutations on the proximal side affect calcium binding of the distal side.
Related PDB
Related UniProtKB
[29]
Resource
Comments
Medline ID
PubMed ID 10074376
Journal Biochemistry
Year 1999
Volume 38
Pages 3205-10
Authors Banci L, Ciofi-Baffoni S, Tien M
Title Lignin and Mn peroxidase-catalyzed oxidation of phenolic lignin oligomers.
Related PDB
Related UniProtKB
[30]
Resource
Comments
Medline ID
PubMed ID 10471300
Journal Biochemistry
Year 1999
Volume 38
Pages 11482-9
Authors Sollewijn Gelpke MD, Moenne-Loccoz P, Gold MH
Title Arginine 177 is involved in Mn(II) binding by manganese peroxidase.
Related PDB
Related UniProtKB
[31]
Resource
Comments
Medline ID
PubMed ID 10413489
Journal Biochemistry
Year 1999
Volume 38
Pages 9146-57
Authors Wang X, Lu Y
Title Proton NMR investigation of the heme active site structure of an engineered cytochrome c peroxidase that mimics manganese peroxidase.
Related PDB
Related UniProtKB
[32]
Resource
Comments
Medline ID
PubMed ID 11051101
Journal Arch Biochem Biophys
Year 2000
Volume 382
Pages 89-94
Authors Ambert-Balay K, Dougherty M, Tien M
Title Reactivity of manganese peroxidase: site-directed mutagenesis of residues in proximity to the porphyrin ring.
Related PDB
Related UniProtKB
[33]
Resource
Comments
Medline ID
PubMed ID 10620333
Journal Arch Biochem Biophys
Year 2000
Volume 373
Pages 147-53
Authors Timofeevski SL, Nie G, Reading NS, Aust SD
Title Substrate specificity of lignin peroxidase and a S168W variant of manganese peroxidase.
Related PDB
Related UniProtKB
[34]
Resource
Comments
Medline ID
PubMed ID 10835231
Journal Biotechnol Prog
Year 2000
Volume 16
Pages 326-33
Authors Reading NS, Aust SD
Title Engineering a disulfide bond in recombinant manganese peroxidase results in increased thermostability.
Related PDB
Related UniProtKB
[35]
Resource
Comments
Medline ID
PubMed ID 11106414
Journal Eur J Biochem
Year 2000
Volume 267
Pages 7038-45
Authors Gelpke MD, Youngs HL, Gold MH
Title Role of arginine 177 in the MnII binding site of manganese peroxidase. Studies with R177D, R177E, R177N, and R177Q mutants.
Related PDB
Related UniProtKB
[36]
Resource
Comments
Medline ID
PubMed ID 10806381
Journal Eur J Biochem
Year 2000
Volume 267
Pages 2840-9
Authors Zou P, Schrempf H
Title The heme-independent manganese-peroxidase activity depends on the presence of the C-terminal domain within the Streptomyces reticuli catalase-peroxidase CpeB.
Related PDB
Related UniProtKB
[37]
Resource
Comments
Medline ID
PubMed ID 10693145
Journal Met Ions Biol Syst
Year 2000
Volume 37
Pages 559-86
Authors Gold MH, Youngs HL, Gelpke MD
Title Manganese peroxidase.
Related PDB
Related UniProtKB
[38]
Resource
Comments
Medline ID
PubMed ID 11329293
Journal Biochemistry
Year 2001
Volume 40
Pages 2243-50
Authors Youngs HL, Sollewijn Gelpke MD, Li D, Sundaramoorthy M, Gold MH
Title The role of Glu39 in MnII binding and oxidation by manganese peroxidase from Phanerochaete chrysoporium.
Related PDB
Related UniProtKB
[39]
Resource
Comments
Medline ID
PubMed ID 11734216
Journal FEBS Lett
Year 2001
Volume 509
Pages 111-4
Authors Miyazaki C, Takahashi H
Title Engineering of the H2O2-binding pocket region of a recombinant manganese peroxidase to be resistant to H2O2.
Related PDB
Related UniProtKB
[40]
Resource
Comments
Medline ID
PubMed ID 12089036
Journal Appl Environ Microbiol
Year 2002
Volume 68
Pages 3514-21
Authors Schlosser D, Hofer C
Title Laccase-catalyzed oxidation of Mn(2+) in the presence of natural Mn(3+) chelators as a novel source of extracellular H(2)O(2) production and its impact on manganese peroxidase.
Related PDB
Related UniProtKB
[41]
Resource
Comments
Medline ID
PubMed ID 12172033
Journal Plant Cell
Year 2002
Volume 14
Pages 1953-62
Authors Onnerud H, Zhang L, Gellerstedt G, Henriksson G
Title Polymerization of monolignols by redox shuttle-mediated enzymatic oxidation: a new model in lignin biosynthesis I.
Related PDB
Related UniProtKB
[42]
Resource
Comments
Medline ID
PubMed ID 12400090
Journal Biopolymers
Year 2003
Volume 72
Pages 38-47
Authors Banci L, Bartalesi I, Ciofi-Baffoni S, Tien M
Title Unfolding and pH studies on manganese peroxidase: role of heme and calcium on secondary structure stability.
Related PDB
Related UniProtKB
[43]
Resource
Comments
Medline ID
PubMed ID 15766255
Journal Biochemistry
Year 2005
Volume 44
Pages 4267-74
Authors Pogni R, Baratto MC, Giansanti S, Teutloff C, Verdin J, Valderrama B, Lendzian F, Lubitz W, Vazquez-Duhalt R, Basosi R
Title Tryptophan-based radical in the catalytic mechanism of versatile peroxidase from Bjerkandera adusta.
Related PDB
Related UniProtKB
[44]
Resource
Comments
Medline ID
PubMed ID 15850380
Journal Biochemistry
Year 2005
Volume 44
Pages 6463-70
Authors Sundaramoorthy M, Youngs HL, Gold MH, Poulos TL
Title High-resolution crystal structure of manganese peroxidase: substrate and i
Related PDB 1yyd 1yyg 1yzp 1yzr
Related UniProtKB

Comments
Although this enzyme binds two calcium ions, they are not involved in catalysis.
According to the literature, this enzyme catalyzes three reactions as follows:
(1) P-Fe3+ + H2O2 = Compound I + H2O ; Compound I = [P+-Fe4+=O] (two-electron transfer)
(2) Compound I + Mn2+ = Compound II + Mn3+ ; Compound II = [P-Fe4+=O] (one-electron transfer)
(3) Compound II + Mn2+ = P-Fe3+ + Mn3+ (one-electron transfer)
Here, "P" and "P+" indicate "a porphyrin" and "a porphyrin pi cation radical", respectively.
Thus, Compound I is a two-electron-oxidized intermediate, containing an oxyferryl center, Fe4+=O, and a porphyrin pi cation radical. Compound II is one-electron-oxidized intermediate, containing an oxyferryl center, Fe4+=O, and a porphyrin.
During the reaction (2), this enzyme can oxidze a phenolic or aromatic amine substrate, such as lignin, instead of manganese (see [37]).

Created Updated
2005-06-02 2009-02-26