DB code: D00059

CATH domain 1.20.144.10 : Vanadium-containing Chloroperoxidase; domain 1
1.10.606.10 : Vanadium-containing Chloroperoxidase; domain 2 Catalytic domain
E.C. 1.11.1.10
CSA 1vnc
M-CSA 1vnc
MACiE M0014

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
P49053 Vanadium chloroperoxidase
EC 1.11.1.10
Vanadium chloride peroxidase
VCPO
PF01569 (PAP2)
[Graphical View]

KEGG enzyme name
chloride peroxidase
chloroperoxidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P49053 PRXC_CURIN RH + Cl(-) + H(2)O(2) = RCl + 2 H(2)O. Secreted. Vanadium.

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C06267 C01371 C00698 C00027 C01334 C00001
E.C.
Compound Vanadium RH Cl- H2O2 RCl H2O
Type heavy metal lipid halide others halide H2O
ChEBI 17996
 17996
 		16240
 16240
 		15377
 15377
PubChem 312
 312
 		22326046
 784
 22326046
 784
 		22247451
 962
 22247451
 962
1idqA01 Unbound Unbound Unbound Unbound Unbound
1iduA01 Unbound Unbound Unbound Unbound Unbound
1vncA01 Unbound Unbound Unbound Unbound Unbound
1vneA01 Unbound Unbound Unbound Unbound Unbound
1vnfA01 Unbound Unbound Unbound Unbound Unbound
1vngA01 Unbound Unbound Unbound Unbound Unbound
1vnhA01 Unbound Unbound Unbound Unbound Unbound
1vniA01 Unbound Unbound Unbound Unbound Unbound
1vnsA01 Unbound Unbound Unbound Unbound Unbound
1idqA02 Bound:VO4 Unbound Unbound Unbound Unbound
1iduA02 Bound:VO4 Unbound Unbound Unbound Unbound
1vncA02 Bound:VO4 Unbound Unbound Unbound Unbound
1vneA02 Bound:VO4 Unbound Unbound Unbound Unbound
1vnfA02 Bound:VO4 Unbound Unbound Unbound Unbound
1vngA02 Bound:VO4 Unbound Unbound Unbound Unbound
1vnhA02 Bound:VO4 Unbound Unbound Unbound Unbound
1vniA02 Bound:VO4 Unbound Unbound Unbound Unbound
1vnsA02 Analogue:SO4 Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P49053 & literature [4], [6], [10]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1idqA01
1iduA01
1vncA01
1vneA01
1vnfA01
1vngA01
1vnhA01
1vniA01
1vnsA01
1idqA02 LYS 353;ARG 360;HIS 404;ARG 490 HIS 496(Vanadium binding)
1iduA02 LYS 353;ARG 360;HIS 404;ARG 490 HIS 496(Vanadium binding)
1vncA02 LYS 353;ARG 360;HIS 404;ARG 490 HIS 496(Vanadium binding)
1vneA02 LYS 353;ARG 360;HIS 404;ARG 490 HIS 496(Vanadium binding) mutant D292A
1vnfA02 LYS 353;;HIS 404;ARG 490 HIS 496(Vanadium binding) mutant R360A
1vngA02 LYS 353;ARG 360;;ARG 490 HIS 496(Vanadium binding) mutant H404A
1vnhA02 LYS 353;ARG 360;HIS 404;ARG 490 mutant H496A
1vniA02 LYS 353;ARG 360;HIS 404;ARG 490 HIS 496(Vanadium binding)
1vnsA02 LYS 353;ARG 360;HIS 404;ARG 490 HIS 496(Vanadium binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[1]
p.395-396
[2]
Fig.7, p.314
[5]
Fig.2a, p.30-31
[6]
Fig.5, p.23825-23826
[8]
Fig.10, p.603-604
[9]
Fig.8, p.11656
[12]
Fig.1, p.294

References
[1]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)
Medline ID 96133943
PubMed ID 8552646
Journal Proc Natl Acad Sci U S A
Year 1996
Volume 93
Pages 392-6
Authors Messerschmidt A, Wever R
Title X-ray structure of a vanadium-containing enzyme: chloroperoxidase from the fungus Curvularia inaequalis.
Related PDB 1vnc
Related UniProtKB P49053
[2]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 9165086
Journal Biol Chem
Year 1997
Volume 378
Pages 309-15
Authors Messerschmidt A, Prade L, Wever R
Title Implications for the catalytic mechanism of the vanadium-containing enzyme chloroperoxidase from the fungus Curvularia inaequalis by X-ray structures of the native and peroxide form.
Related PDB 1idu 1idq 1vne 1vnf
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 9224681
Journal FEBS Lett
Year 1997
Volume 409
Pages 317-9
Authors Hemrika W, Wever R
Title A new model for the membrane topology of glucose-6-phosphatase: the enzyme involved in von Gierke disease.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 9260289
Journal Protein Sci
Year 1997
Volume 6
Pages 1764-7
Authors Neuwald AF
Title An unexpected structural relationship between integral membrane phosphatases and soluble haloperoxidases.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 10021409
Journal Curr Opin Chem Biol
Year 1999
Volume 3
Pages 28-34
Authors Littlechild J
Title Haloperoxidases and their role in biotransformation reactions.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 10446144
Journal J Biol Chem
Year 1999
Volume 274
Pages 23820-7
Authors Hemrika W, Renirie R, Macedo-Ribeiro S, Messerschmidt A, Wever R
Title Heterologous expression of the vanadium-containing chloroperoxidase from Curvularia inaequalis in Saccharomyces cerevisiae and site-directed mutagenesis of the active site residues His(496), Lys(353), Arg(360), and Arg(490).
Related PDB
Related UniProtKB
[7]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10499093
Journal J Biol Inorg Chem
Year 1999
Volume 4
Pages 209-19
Authors Macedo-Ribeiro S, Hemrika W, Renirie R, Wever R, Messerschmidt A
Title X-ray crystal structures of active site mutants of the vanadium-containing chloroperoxidase from the fungus Curvularia inaequalis.
Related PDB 1vng 1vnh 1vni 1vns
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 10543953
Journal J Mol Biol
Year 1999
Volume 293
Pages 595-611
Authors Weyand M, Hecht H, Kiess M, Liaud M, Vilter H, Schomburg D
Title X-ray structure determination of a vanadium-dependent haloperoxidase from Ascophyllum nodosum at 2.0 A resolution.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 10766783
Journal J Biol Chem
Year 2000
Volume 275
Pages 11650-7
Authors Renirie R, Hemrika W, Wever R
Title Peroxidase and phosphatase activity of active-site mutants of vanadium chloroperoxidase from the fungus Curvularia inaequalis. Implications for the catalytic mechanisms.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 10843856
Journal J Mol Biol
Year 2000
Volume 299
Pages 1035-49
Authors Isupov MN, Dalby AR, Brindley AA, Izumi Y, Tanabe T, Murshudov GN, Littlechild JA
Title Crystal structure of dodecameric vanadium-dependent bromoperoxidase from the red algae Corallina officinalis.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 11800602
Journal Inorg Chem
Year 2002
Volume 41
Pages 161-3
Authors Kimblin C, Bu X, Butler A
Title Modeling the catalytic site of vanadium bromoperoxidase: synthesis and structural characterization of intramolecularly H-bonded vanadium(V) oxoperoxo complexes, [VO(O(2))((NH)2pyg(2))]K and [VO(O(2))((BrNH)2pyg(2))]K.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 12447906
Journal J Mol Recognit
Year 2002
Volume 15
Pages 291-6
Authors Littlechild J, Garcia-Rodriguez E, Dalby A, Isupov M
Title Structural and functional comparisons between vanadium haloperoxidase and acid phosphatase enzymes.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-06-21 2009-02-26