DB code: T00258

RLCP classification 3.105.250000.90 : Transfer
CATH domain -.-.-.- :
3.40.50.- : Rossmann fold
3.40.930.10 : Mannitol-specific EII; Chain A Catalytic domain
E.C. 2.7.1.-

CATH domain Related DB codes (homologues)
3.40.930.10 : Mannitol-specific EII; Chain A S00420

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Includes RefSeq Pfam
P00550 PTS system mannitol-specific EIICBA component
Mannitol permease IIC component PTS system mannitol-specific EIIC component
Mannitol-specific phosphotransferase enzyme IIB component
PTS system mannitol-specific EIIB component
Mannitol-specific phosphotransferase enzyme IIA component
EC 2.7.1.-
PTS system mannitol-specific EIIA component
NP_418056.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491835.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF00359 (PTS_EIIA_2)
PF02378 (PTS_EIIC)
PF02302 (PTS_IIB)
[Graphical View]

KEGG enzyme name
protein-Npi-phosphohistidine---sugar phosphotransferase
(EC )
glucose permease
(EC )
PTS permease
(EC )
phosphotransferase, phosphohistidinoprotein-hexose
(EC )
enzyme IIl4ac
(EC )
gene glC proteins
(EC )
gene bglC RNA formation factors
(EC )
PEP-dependent phosphotransferase enzyme II
(EC )
PEP-sugar phosphotransferase enzyme II
(EC )
phosphoenolpyruvate-sugar phosphotransferase enzyme II
(EC )
phosphohistidinoprotein-hexose phosphotransferase
(EC )
phosphohistidinoprotein-hexose phosphoribosyltransferase
(EC )
phosphoprotein factor-hexose phosophotransferase
(EC )
protein, specific or class, gene bglC
(EC )
ribonucleic acid formation factor, gene glC
(EC )
sucrose phosphotransferase system II
(EC )
protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase
(EC )
protein-Npi-phosphohistidine:sugar Npi-phosphotransferase
(EC )

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P00550 PTM3C_ECOLI Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L- histidine/cysteine. Protein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate. Homodimer. Cell inner membrane, Multi-pass membrane protein.

KEGG Pathways
Map code Pathways E.C.
MAP00010 Glycolysis / Gluconeogenesis
MAP00051 Fructose and mannose metabolism
MAP00052 Galactose metabolism
MAP00053 Ascorbate and aldarate metabolism
MAP00500 Starch and sucrose metabolism
MAP00530 Aminosugars metabolism

Compound table
Substrates Products Intermediates
KEGG-id C04261 C11477 C02743 C00615 C00934 L00031
Compound Protein N(pi)-phospho-L-histidine Sugar Protein cysteine Protein histidine Sugar phosphate Protein S-phosphoryl-cysteine
Type aromatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ion polysaccharide peptide/protein,sulfhydryl group aromatic ring (with nitrogen atoms),peptide/protein phosphate group/phosphate ion,polysaccharide peptide/protein,phosphate group/phosphate ion,sulfide group
1a3aA Unbound Unbound Unbound Unbound Unbound Unbound
1a3aB Unbound Unbound Unbound Unbound Unbound Unbound
1a3aC Unbound Unbound Unbound Unbound Unbound Unbound
1a3aD Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1a3aA ARG 49;HIS 65;HIS 111 HIS 65 (phosphorylated)
1a3aB ARG 49;HIS 65;HIS 111 HIS 65 (phosphorylated)
1a3aC ARG 49;HIS 65;HIS 111 HIS 65 (phosphorylated)
1a3aD ARG 49;HIS 65;HIS 111 HIS 65 (phosphorylated)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
Fig.7, Fig.8, p.385 2

Medline ID
PubMed ID 1946374
Journal Proc Natl Acad Sci U S A
Year 1991
Volume 88
Pages 9603-7
Authors Sugiyama JE, Mahmoodian S, Jacobson GR
Title Membrane topology analysis of Escherichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions.
Related PDB
Related UniProtKB P00550
Medline ID
PubMed ID 9551558
Journal Structure
Year 1998
Volume 6
Pages 377-88
Authors van Montfort RL, Pijning T, Kalk KH, Hangyi I, Kouwijzer ML, Robillard GT, Dijkstra BW
Title The structure of the Escherichia coli phosphotransferase IIAmannitol reveals a novel fold with two conformations of the active site.
Related PDB 1a3a
Related UniProtKB P00550
Medline ID
PubMed ID 9636714
Journal J Mol Biol
Year 1998
Volume 279
Pages 245-55
Authors Bordo D, van Monfort RL, Pijning T, Kalk KH, Reizer J, Saier MH Jr, Dijkstra BW
Title The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli.
Related PDB 1a6j
Related UniProtKB P69829

This enzyme is composed of three domains: The N-terminal domain for Mannitol permease IIC component, a domain for Mannitol-specific phosphotransferase enzyme IIB component (E.C., and the C-terminal domain for Mannitol-specific phosphotransferase enzyme IIA component (E.C. 2.7.1.-).
The same E.C. number ( appears in S00297, D00527, S00283, S00046. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, a phosphoryl group is transferred from phosphoenolpyruvate (PEP) via the PTS enzymes, EI, HPr, IIA, IIB to the tranported sugar. The enzyme here is IIA subunit of a mannitol transporter (IIA-mannitol).
His65 (1a3a) is phosphorylated during the phosphotransfer reaction. A phosphoryl transfer between two PTS proteins involves an in-line attack of the phosphorus atom by the acceptor protein. A trigonal bipyramidal transition state is formed with a pentavalent coordination of the phosphorus atom. Productive breakdown of the transition state results in an inversion of the configuration at the phosphorus atom [2]. Although there is no biochemical evidence for the roles of Arg49/His111 (1a3a), Arg49 is ideally positioned to stabilize the transition state in the phosphoryl transfer from HPr to IIA-mannitol. In contrast, whilst His111 is pointed away from the phosphorylation site for HPr, it might also be needed for the phosphoryl transfer to IIB-mannitol.

Created Updated
2002-08-30 2009-03-04