DB code: T00100

CATH domain 3.40.50.1070 : Rossmann fold
3.40.275.10 : L-fucose Isomerase; Chain A, domain 2 Catalytic domain
3.20.14.10 : L-fucose Isomerase; Chain A, domain 3 Catalytic domain
E.C. 5.3.1.25
CSA 1fui
M-CSA 1fui
MACiE M0095

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P69922 L-fucose isomerase
EC 5.3.1.25
6-deoxy-L-galactose isomerase
FucIase
NP_417282.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491010.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF02952 (Fucose_iso_C)
PF07881 (Fucose_iso_N1)
PF07882 (Fucose_iso_N2)
[Graphical View]

KEGG enzyme name
L-fucose isomerase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P69922 FUCI_ECOLI L-fucose = L-fuculose. Homohexamer. Cytoplasm. Manganese.

KEGG Pathways
Map code Pathways E.C.
MAP00051 Fructose and mannose metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00034 C01019 C01721
E.C.
Compound Manganese L-Fucose L-Fuculose
Type heavy metal carbohydrate carbohydrate
ChEBI 18291
 35154
 18291
 35154
 		2181
 2181
 		17617
 17617
PubChem 23930
 23930
 		17106
 17106
 		6857362
 6857362
1fuiA01 Unbound Unbound Unbound
1fuiB01 Unbound Unbound Unbound
1fuiC01 Unbound Unbound Unbound
1fuiD01 Unbound Unbound Unbound
1fuiE01 Unbound Unbound Unbound
1fuiF01 Unbound Unbound Unbound
1fuiA02 Unbound Unbound Unbound
1fuiB02 Unbound Unbound Unbound
1fuiC02 Unbound Unbound Unbound
1fuiD02 Unbound Unbound Unbound
1fuiE02 Unbound Unbound Unbound
1fuiF02 Unbound Unbound Unbound
1fuiA03 Bound:_MN Analogue:FOC Unbound
1fuiB03 Bound:_MN Analogue:FOC Unbound
1fuiC03 Bound:_MN Analogue:FOC Unbound
1fuiD03 Bound:_MN Analogue:FOC Unbound
1fuiE03 Bound:_MN Analogue:FOC Unbound
1fuiF03 Unbound Analogue:FOC Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P69922 & literature [7]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1fuiA01
1fuiB01
1fuiC01
1fuiD01
1fuiE01
1fuiF01
1fuiA02 GLU 337 GLU 337(Manganese binding)
1fuiB02 GLU 337 GLU 337(Manganese binding)
1fuiC02 GLU 337 GLU 337(Manganese binding)
1fuiD02 GLU 337 GLU 337(Manganese binding)
1fuiE02 GLU 337 GLU 337(Manganese binding)
1fuiF02 GLU 337 GLU 337(Manganese binding)
1fuiA03 ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiB03 ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiC03 ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiD03 ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiE03 ASP 361 ASP 361;HIS 528(Manganese binding)
1fuiF03 ASP 361 ASP 361;HIS 528(Manganese binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[7]
Fig.11, p.260-262, p.264-266
[10]
Scheme 16, p.9-10
[11]
p.101

References
[1]
Resource
Comments
Medline ID
PubMed ID 13319278
Journal J Biol Chem
Year 1956
Volume 219
Pages 557-68
Authors GREEN M, COHEN SS
Title Enzymatic conversion of L-fucose to L-fuculose.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID
Journal Methods Enzymol
Year 1966
Volume 9
Pages 583-5
Authors Mortlock RP
Title D-Arabinose isomerase.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 4632320
Journal J Bacteriol
Year 1973
Volume 113
Pages 687-96
Authors Boulter JR, Gielow WO
Title Properties of D-arabinose isomerase purified from two strains of Escherichia coli.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID
Journal Agric Biol Chem
Year 1974
Volume 38
Pages 267-73
Authors Izumori K, Yamanaka K
Title Purification and crystallization of D-arabinose (L-fucose) isomerase from Aerobacter aerogenes by polyethylene glycol.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID
Journal Agric Biol Chem
Year 1976
Volume 40
Pages 439-440
Authors Yamanaka K, Izumori K
Title Inhibition of D-arabinose (L-fucose) isomerase activity by Tris and its analogues.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID
Journal Agric Biol Chem
Year 1979
Volume 43
Pages 1993-4
Authors Izumori K, Yamanaka K
Title Activation and inhibition of D-arabinose isomerase of Klebsiella aerogenes by L-histidine.
Related PDB
Related UniProtKB
[7]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
Medline ID 98035055
PubMed ID 9367760
Journal J Mol Biol
Year 1997
Volume 273
Pages 256-68
Authors Seemann JE, Schulz GE
Title Structure and mechanism of L-fucose isomerase from Escherichia coli.
Related PDB 1fui
Related UniProtKB P69922
[8]
Resource
Comments
Medline ID
PubMed ID 9485504
Journal Proteins
Year 1997
Volume Suppl 1
Pages 129-33
Authors Flockner H, Domingues FS, Sippl MJ
Title Protein folds from pair interactions: a blind test in fold recognition.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 10368289
Journal Structure Fold Des
Year 1999
Volume 7
Pages 227-36
Authors Castillo RM, Mizuguchi K, Dhanaraj V, Albert A, Blundell TL, Murzin AG
Title A six-stranded double-psi beta barrel is shared by several protein superfamilies.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 11368170
Journal Arch Biochem Biophys
Year 2001
Volume 387
Pages 1-10
Authors Creighton DJ, Hamilton DS
Title Brief history of glyoxalase I and what we have learned about metal ion-dependent, enzyme-catalyzed isomerizations.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID
Journal Top Curr Chem
Year 2001
Volume 215
Pages 77-114
Authors Hausler H, Stutz AE
Title D-xylose (D-glucose) isomerase and related enzymes in carbohydrate synthesis.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 12645017
Journal Chemistry
Year 2003
Volume 9
Pages 1281-95
Authors Casanova D, Alemany P, Bofill JM, Alvarez S
Title Shape and symmetry of heptacoordinate transition-metal complexes: structural trends.
Related PDB
Related UniProtKB

Comments

Created Updated
2005-07-04 2009-02-26