DB code: S00387
CATH domain | 3.40.109.10 : NADH Oxidase | Catalytic domain |
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E.C. | 1.-.-.- | |
CSA | ||
M-CSA | ||
MACiE |
CATH domain | Related DB codes (homologues) |
---|---|
3.40.109.10 : NADH Oxidase | S00385 S00386 S00388 |
Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
---|---|---|---|---|
P17117 |
Oxygen-insensitive NADPH nitroreductase
|
EC
1.-.-.-
Modulator of drug activity A |
NP_415372.1
(Protein)
NC_000913.2 (DNA/RNA sequence) YP_489124.1 (Protein) NC_007779.1 (DNA/RNA sequence) |
PF00881
(Nitroreductase)
[Graphical View] |
KEGG enzyme name |
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UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
---|---|---|---|---|---|
P17117 | NFSA_ECOLI | FMN. |
KEGG Pathways | Map code | Pathways | E.C. |
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Compound table | |||||||||||||
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Cofactors | Substrates | Products | Intermediates | ||||||||||
KEGG-id | C00061 | C00005 | C06813 | C00080 | C00006 | C02720 | C00001 | ||||||
E.C. | |||||||||||||
Compound | FMN | NADPH | Nitrobenzene | H+ | NADP+ | N-Hydroxyarylamine | H2O | ||||||
Type | amide group,amine group,aromatic ring (only carbon atom),aromatic ring (with nitrogen atoms),carbohydrate,phosphate group/phosphate ion | amide group,amine group,nucleotide | aromatic ring (only carbon atom),nitro group | others | amide group,amine group,nucleotide | amine group,aromatic ring (only carbon atom) | H2O | ||||||
ChEBI |
17621 17621 |
16474 16474 |
27798 27798 |
15378 15378 |
18009 18009 |
28902 28902 |
15377 15377 |
||||||
PubChem |
643976 643976 |
5884 5884 |
7416 7416 |
1038 1038 |
5886 5886 |
7518 7518 |
22247451 962 22247451 962 |
||||||
1f5vA | Bound:FMN | Unbound | Unbound | Unbound | Unbound | ||||||||
1f5vB | Bound:FMN | Unbound | Unbound | Unbound | Unbound |
Reference for Active-site residues | ||
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resource | references | E.C. |
Active-site residues | ||||||||||
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PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
1f5vA | ||||||||||
1f5vB |
References for Catalytic Mechanism | ||
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References | Sections | No. of steps in catalysis |
[4]
|
p.267 |
References | |
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[1] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 374406 |
Journal | J Biol Chem |
Year | 1979 |
Volume | 254 |
Pages | 4009-14 |
Authors | Peterson FJ, Mason RP, Hovsepian J, Holtzman JL |
Title | Oxygen-sensitive and -insensitive nitroreduction by Escherichia coli and rat hepatic microsomes. |
Related PDB | |
Related UniProtKB | |
[2] | |
Resource | |
Comments | |
Medline ID | |
PubMed ID | 8755878 |
Journal | J Bacteriol |
Year | 1996 |
Volume | 178 |
Pages | 4508-14 |
Authors | Zenno S, Koike H, Kumar AN, Jayaraman R, Tanokura M, Saigo K |
Title |
Biochemical characterization of NfsA, |
Related PDB | |
Related UniProtKB | |
[3] | |
Resource | |
Comments | MUTAGENESIS |
Medline ID | 98101485 |
PubMed ID | 9440535 |
Journal | J Bacteriol |
Year | 1998 |
Volume | 180 |
Pages | 422-5 |
Authors | Zenno S, Kobori T, Tanokura M, Saigo K |
Title |
Conversion of NfsA, |
Related PDB | |
Related UniProtKB | P17117 |
[4] | |
Resource | |
Comments | Homologous enzyme |
Medline ID | |
PubMed ID | 9654450 |
Journal | J Mol Biol |
Year | 1998 |
Volume | 280 |
Pages | 259-73 |
Authors | Koike H, Sasaki H, Kobori T, Zenno S, Saigo K, Murphy ME, Adman ET, Tanokura M |
Title |
1.8 A crystal structure of the major NAD(P)H:FMN oxidoreductase of a bioluminescent bacterium, |
Related PDB | |
Related UniProtKB | |
[5] | |
Resource | |
Comments | X-ray crystallography |
Medline ID | |
PubMed ID | 11034992 |
Journal | J Biol Chem |
Year | 2001 |
Volume | 276 |
Pages | 2816-23 |
Authors | Kobori T, Sasaki H, Lee WC, Zenno S, Saigo K, Murphy ME, Tanokura M |
Title | Structure and site-directed mutagenesis of a flavoprotein from Escherichia coli that reduces nitrocompounds: alteration of pyridine nucleotide binding by a single amino acid substitution. |
Related PDB | 1f5v |
Related UniProtKB |
Comments |
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The substrates and products are annotated based on literature [1].
Although this enzyme is homologous to other NADPH nitrogenase (S00388 in EzCatDB), |
Created | Updated |
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2004-07-12 | 2009-02-26 |