DB code: S00366
| RLCP classification | 3.100.220195.81 : Transfer | |
|---|---|---|
| CATH domain | 3.40.50.1240 : Rossmann fold | Catalytic domain |
| E.C. | 5.4.2.1 | |
| CSA | 1qhf | |
| M-CSA | 1qhf | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.1240 : Rossmann fold | S00365 S00363 D00460 D00514 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P62707 |
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
|
BPG-dependent PGAM
PGAM Phosphoglyceromutase dPGM EC 5.4.2.1 |
NP_415276.1
(Protein)
NC_000913.2 (DNA/RNA sequence) YP_489028.1 (Protein) NC_007779.1 (DNA/RNA sequence) |
PF00300
(His_Phos_1)
[Graphical View] |
| P0A5R6 |
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
|
BPG-dependent PGAM
PGAM Phosphoglyceromutase dPGM EC 5.4.2.1 |
NP_334917.1
(Protein)
NC_002755.2 (DNA/RNA sequence) YP_006513819.1 (Protein) NC_018143.1 (DNA/RNA sequence) YP_177731.1 (Protein) NC_000962.3 (DNA/RNA sequence) |
PF00300
(His_Phos_1)
[Graphical View] |
| Q6MWZ7 |
(Possible phosphoglycerate mutase Gpm2
|
Phosphoglyceromutase
PGAM) (BPG-dependent PGAM) |
YP_006516686.1
(Protein)
NC_018143.1 (DNA/RNA sequence) YP_177944.1 (Protein) NC_000962.3 (DNA/RNA sequence) |
PF00300
(His_Phos_1)
[Graphical View] |
| P36623 |
Phosphoglycerate mutase
|
PGAM
EC 5.4.2.1 BPG-dependent PGAM MPGM Phosphoglyceromutase |
NP_594889.1
(Protein)
NM_001020318.2 (DNA/RNA sequence) |
PF00300
(His_Phos_1)
[Graphical View] |
| P00950 |
Phosphoglycerate mutase 1
|
PGAM 1
EC 5.4.2.1 BPG-dependent PGAM 1 MPGM 1 Phosphoglyceromutase 1 |
NP_012770.1
(Protein)
NM_001179718.1 (DNA/RNA sequence) |
PF00300
(His_Phos_1)
[Graphical View] |
| KEGG enzyme name |
|---|
|
phosphoglycerate mutase
phosphoglycerate phosphomutase phosphoglyceromutase glycerate phosphomutase (diphosphoglycerate cofactor) monophosphoglycerate mutase monophosphoglyceromutase diphosphoglycomutase diphosphoglycerate mutase bisphosphoglyceromutase GriP mutase MPGM PGA mutase PGAM-i PGAM PGAM-d PGM |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P62707 | GPMA_ECOLI | 2-phospho-D-glycerate = 3-phospho-D-glycerate. | Homodimer. | ||
| P0A5R6 | GPMA_MYCTU | 2-phospho-D-glycerate = 3-phospho-D-glycerate. | |||
| Q6MWZ7 | Q6MWZ7_MYCTU | ||||
| P00950 | PMG1_YEAST | 2-phospho-D-glycerate = 3-phospho-D-glycerate. | Homotetramer. | ||
| P36623 | PMGY_SCHPO | 2-phospho-D-glycerate = 3-phospho-D-glycerate. | Monomer. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00010 | Glycolysis / Gluconeogenesis |
| Compound table | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||
| KEGG-id | C01159 | C00631 | C00197 | C04262 | |||||
| E.C. | |||||||||
| Compound | 2,3-Bisphospho-D-glycerate | 2-Phospho-D-glycerate | 3-Phospho-D-glycerate | Protein N(tau)-phospho-L-histidine | |||||
| Type | carboxyl group,phosphate group/phosphate ion | carbohydrate,carboxyl group,phosphate group/phosphate ion | carbohydrate,carboxyl group,phosphate group/phosphate ion | ||||||
| ChEBI |
17720 17720 |
17835 17835 |
17794 17794 |
||||||
| PubChem |
186004 186004 |
439278 439278 |
439183 439183 |
||||||
| 1e58A |
|
|
|
|
|
Unbound | Unbound | Unbound | Intermediate-bound:HIS_10-NEP |
| 1e59A |
|
|
|
|
|
Analogue:VO3 | Unbound | Unbound | Unbound |
| 1riiA |
|
|
|
|
|
Unbound | Analogue:GOL | Unbound | Unbound |
| 1riiB |
|
|
|
|
|
Unbound | Analogue:GOL | Unbound | Unbound |
| 1riiC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 1riiD |
|
|
|
|
|
Unbound | Analogue:GOL | Unbound | Unbound |
| 2a6pA |
|
|
|
|
|
Unbound | Analogue:GOL-SO4 | Unbound | Unbound |
| 2a6pB |
|
|
|
|
|
Unbound | Analogue:GOL-SO4 | Unbound | Unbound |
| 1bq3A |
|
|
|
|
|
Analogue:IHP | Unbound | Unbound | Unbound |
| 1bq3B |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 1bq3C |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 1bq3D |
|
|
|
|
|
Analogue:IHP | Unbound | Unbound | Unbound |
| 1bq4A |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 1bq4B |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 1bq4C |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 1bq4D |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 1qhfA |
|
|
|
|
|
Unbound | Unbound | Bound:3PG | Unbound |
| 1qhfB |
|
|
|
|
|
Unbound | Unbound | Bound:3PG | Unbound |
| 3pgmA |
|
|
|
|
|
Unbound | Unbound | Bound:3PG | Unbound |
| 4pgmA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 4pgmB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 4pgmC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 4pgmD |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 5pgmA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 5pgmB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 5pgmC |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 5pgmD |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 5pgmE |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 5pgmF |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 5pgmG |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 5pgmH |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| 1fztA |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P00950, P62707, P36623 & literature [18] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1e58A |
|
|
|
|
|
HIS 10;GLU 88;ARG 9;ASN 16;ARG 61;HIS 183 | HIS 10(Phosphorylation) | Modified residue H10-NEP | ||
| 1e59A |
|
|
|
|
|
HIS 10;GLU 88;ARG 9;ASN 16;ARG 61;HIS 183 | HIS 10(Phosphorylation) | |||
| 1riiA |
|
|
|
|
|
HIS 12;GLU 90;ARG 11;ASN 18;ARG 63;HIS 183 | HIS 12(Phosphorylation) | |||
| 1riiB |
|
|
|
|
|
HIS 12;GLU 90;ARG 11;ASN 18;ARG 63;HIS 183 | HIS 12(Phosphorylation) | |||
| 1riiC |
|
|
|
|
|
HIS 12;GLU 90;ARG 11;ASN 18;ARG 63;HIS 183 | HIS 12(Phosphorylation) | |||
| 1riiD |
|
|
|
|
|
HIS 12;GLU 90;ARG 11;ASN 18;ARG 63;HIS 183 | HIS 12(Phosphorylation) | |||
| 2a6pA |
|
|
|
|
|
HIS 13;GLU 85;ARG 12; ;ARG 64;HIS 147 | HIS 13(Phosphorylation) | |||
| 2a6pB |
|
|
|
|
|
HIS 13;GLU 85;ARG 12; ;ARG 64;HIS 147 | HIS 13(Phosphorylation) | |||
| 1bq3A |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1bq3B |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1bq3C |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1bq3D |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1bq4A |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1bq4B |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1bq4C |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1bq4D |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1qhfA |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1qhfB |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 3pgmA |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 179 | HIS 8(Phosphorylation) | |||
| 4pgmA |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 4pgmB |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 4pgmC |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 4pgmD |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 5pgmA |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 5pgmB |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 5pgmC |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 5pgmD |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 5pgmE |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 5pgmF |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 5pgmG |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 5pgmH |
|
|
|
|
|
HIS 8;GLU 86;ARG 7;ASN 14;ARG 59;HIS 181 | HIS 8(Phosphorylation) | |||
| 1fztA |
|
|
|
|
|
HIS 15;GLU 93;ARG 14;ASN 21;ARG 66;HIS 163 | HIS 15(Phosphorylation) | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[2]
|
Scheme II, p.741-742 | |
|
[4]
|
Fig.3, p.125-128 | |
|
[14]
|
p.455-457 | |
|
[15]
|
p.1825-1826 | |
|
[18]
|
Fig.5, p.1511-1515 | |
|
[23]
|
p.1076-1077 | |
|
[24]
|
p.1133-1137 | |
|
[26]
|
p.82-83 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6251799 |
| Journal | Biochem Biophys Res Commun |
| Year | 1980 |
| Volume | 95 |
| Pages | 132-9 |
| Authors | Hass LF, Miller KB |
| Title | Human erythrocyte phosphoglycerate mutase: evidence for normal catalysis in the absence of added 2,3-bisphospho-D-glycerate. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6243955 |
| Journal | Biochemistry |
| Year | 1980 |
| Volume | 19 |
| Pages | 738-43 |
| Authors | Blattler WA, Knowles JR |
| Title | Phosphoglycerate mutases: stereochemical course of the phosphoryl group transfers catalyzed by the cofactor-dependent enzyme from rabbit muscle and the cofactor-independent enzyme from wheat germ. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6115417 |
| Journal | Philos Trans R Soc Lond B Biol Sci |
| Year | 1981 |
| Volume | 293 |
| Pages | 177-89 |
| Authors | Sternberg MJ, Cohen FE, Taylor WR, Feldmann RJ |
| Title | Analysis and predication of structural motifs in the glycolytic enzymes. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS). |
| Medline ID | 81273329 |
| PubMed ID | 6115412 |
| Journal | Philos Trans R Soc Lond B Biol Sci |
| Year | 1981 |
| Volume | 293 |
| Pages | 121-30 |
| Authors | Winn SI, Watson HC, Harkins RN, Fothergill LA |
| Title | Structure and activity of phosphoglycerate mutase. |
| Related PDB | 3pgm |
| Related UniProtKB | P00950 |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6282653 |
| Journal | Fed Proc |
| Year | 1982 |
| Volume | 41 |
| Pages | 2424-31 |
| Authors | Knowles JR |
| Title | Phospho transfer enzymes: lessons from stereochemistry. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6318807 |
| Journal | Biochemistry |
| Year | 1983 |
| Volume | 22 |
| Pages | 6130-4 |
| Authors | Hunt AG, Simplaceanu V, Hong JS, Ho C |
| Title | Phosphorus-31 nuclear magnetic resonance investigation of membrane vesicles from Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6331428 |
| Journal | Biochem Biophys Res Commun |
| Year | 1984 |
| Volume | 121 |
| Pages | 826-33 |
| Authors | Amato SV, Rose ZB, Liebman MN |
| Title | Macro-structural organization of phosphoglycerate mutase. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6331429 |
| Journal | Biochem Biophys Res Commun |
| Year | 1984 |
| Volume | 121 |
| Pages | 834-41 |
| Authors | Rose ZB, Kaklij GS |
| Title | The effects of anions on phosphoglycerate mutase. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1659318 |
| Journal | Arch Biochem Biophys |
| Year | 1991 |
| Volume | 291 |
| Pages | 201-11 |
| Authors | Mendz GL |
| Title | Stimulation of mutases and isomerases by vanadium. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8110200 |
| Journal | Biochem J |
| Year | 1994 |
| Volume | 297 |
| Pages | 603-8 |
| Authors | Nairn J, Price NC, Fothergill-Gilmore LA, Walker GE, Fothergill JE, Dunbar B |
| Title | The amino acid sequence of the small monomeric phosphoglycerate mutase from the fission yeast Schizosaccharomyces pombe. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8765231 |
| Journal | Biochim Biophys Acta |
| Year | 1996 |
| Volume | 1296 |
| Pages | 69-75 |
| Authors | Nairn J, Price NC, Kelly SM, Rigden D, Fothergill-Gilmore LA, Krell T |
| Title | Phosphoglycerate mutase from Schizosaccharomyces pombe: development of an expression system and characterisation of three histidine mutants of the enzyme. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9030753 |
| Journal | Eur J Biochem |
| Year | 1997 |
| Volume | 243 |
| Pages | 306-14 |
| Authors | Seip S, Lanz R, Gutknecht R, Flukiger K, Erni B |
| Title | The fructose transporter of Bacillus subtilis encoded by the lev operon: backbone assignment and secondary structure of the IIB(Lev) subunit. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9390410 |
| Journal | J Biomol NMR |
| Year | 1997 |
| Volume | 10 |
| Pages | 309-10 |
| Authors | Uhrinova S, Uhrin D, Nairn J, Price NC, Fothergill-Gilmore LA, Barlow PN |
| Title | Backbone assignment of double labelled 23.7 kDa phosphoglycerate mutase from Schizosaccharomyces pombe. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS). |
| Medline ID | 98173871 |
| PubMed ID | 9512715 |
| Journal | J Mol Biol |
| Year | 1998 |
| Volume | 276 |
| Pages | 449-59 |
| Authors | Rigden DJ, Alexeev D, Phillips SE, Fothergill-Gilmore LA |
| Title |
The 2.3 A X-ray crystal structure of S. |
| Related PDB | 4pgm |
| Related UniProtKB | P00950 |
| [15] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10531478 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1999 |
| Volume | 55 |
| Pages | 1822-6 |
| Authors | Crowhurst GS, Dalby AR, Isupov MN, Campbell JW, Littlechild JA |
| Title | Structure of a phosphoglycerate mutase:3-phosphoglyceric acid complex at 1.7 A. |
| Related PDB | 1qhf |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10437801 |
| Journal | FEBS Lett |
| Year | 1999 |
| Volume | 455 |
| Pages | 344-8 |
| Authors | Fraser HI, Kvaratskhelia M, White MF |
| Title | The two analogous phosphoglycerate mutases of Escherichia coli. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10369755 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 289 |
| Pages | 691-9 |
| Authors | Rigden DJ, Walter RA, Phillips SE, Fothergill-Gilmore LA |
| Title | Polyanionic inhibitors of phosphoglycerate mutase: combined structural and biochemical analysis. |
| Related PDB | 1bq3 1bq4 |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10064712 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 286 |
| Pages | 1507-17 |
| Authors | Rigden DJ, Walter RA, Phillips SE, Fothergill-Gilmore LA |
| Title |
Sulphate ions observed in the 2.12 A structure of a new crystal form of S. |
| Related PDB | 5pgm |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11106417 |
| Journal | Eur J Biochem |
| Year | 2000 |
| Volume | 267 |
| Pages | 7065-74 |
| Authors | Nairn J, Duncan D, Price NE, Kelly SM, Fothergill-Gilmore LA, Uhrinova S, Barlow PN, Rigden DJ, Price NC |
| Title |
Characterization of active-site mutants of Schizosaccharomyces pombe phosphoglycerate mutase. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 11038361 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 3247-53 |
| Authors | Bond CS, White MF, Hunter WN |
| Title | High resolution structure of the phosphohistidine-activated form of Escherichia coli cofactor-dependent phosphoglycerate mutase. |
| Related PDB | 1e58 |
| Related UniProtKB | P62707 |
| [21] | |
| Resource | |
| Comments |
NMR, |
| Medline ID | |
| PubMed ID | 11237600 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 306 |
| Pages | 275-90 |
| Authors | Uhrinova S, Uhrin D, Nairn J, Price NC, Fothergill-Gilmore LA, Barlow PN |
| Title |
Solution structure and dynamics of an open beta-sheet, |
| Related PDB | 1fzt |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11514674 |
| Journal | Protein Sci |
| Year | 2001 |
| Volume | 10 |
| Pages | 1835-46 |
| Authors | Rigden DJ, Bagyan I, Lamani E, Setlow P, Jedrzejas MJ |
| Title | A cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus is actually a broad specificity phosphatase. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS). |
| Medline ID | |
| PubMed ID | 11884145 |
| Journal | J Mol Biol |
| Year | 2002 |
| Volume | 316 |
| Pages | 1071-81 |
| Authors | Bond CS, White MF, Hunter WN |
| Title | Mechanistic implications for Escherichia coli cofactor-dependent phosphoglycerate mutase based on the high-resolution crystal structure of a vanadate complex. |
| Related PDB | 1e59 |
| Related UniProtKB | P62707 |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11827481 |
| Journal | J Mol Biol |
| Year | 2002 |
| Volume | 315 |
| Pages | 1129-43 |
| Authors | Rigden DJ, Mello LV, Setlow P, Jedrzejas MJ |
| Title | Structure and mechanism of action of a cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus with broad specificity phosphatase activity. |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11951058 |
| Journal | Med Sci Monit |
| Year | 2002 |
| Volume | 8 |
| Pages | BR123-35 |
| Authors | Torshin IY |
| Title | Functional maps of the junctions between interglobular contacts and active sites in glycolytic enzymes -- a comparative analysis of the biochemical and structural data. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12586342 |
| Journal | FEBS Lett |
| Year | 2003 |
| Volume | 536 |
| Pages | 77-84 |
| Authors | Rigden DJ |
| Title | Unexpected catalytic site variation in phosphoprotein phosphatase homologues of cofactor-dependent phosphoglycerate mutase. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15258155 |
| Journal | J Biol Chem |
| Year | 2004 |
| Volume | 279 |
| Pages | 39132-8 |
| Authors | Wang Y, Wei Z, Bian Q, Cheng Z, Wan M, Liu L, Gong W |
| Title | Crystal structure of human bisphosphoglycerate mutase. |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15735341 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 2005 |
| Volume | 61 |
| Pages | 309-15 |
| Authors | Muller P, Sawaya MR, Pashkov I, Chan S, Nguyen C, Wu Y, Perry LJ, Eisenberg D |
| Title | The 1.70 angstroms X-ray crystal structure of Mycobacterium tuberculosis phosphoglycerate mutase. |
| Related PDB | 1rii |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 16672613 |
| Journal | J Bacteriol |
| Year | 2006 |
| Volume | 188 |
| Pages | 3589-99 |
| Authors | Watkins HA, Baker EN |
| Title |
Structural and functional analysis of Rv3214 from Mycobacterium tuberculosis, |
| Related PDB | 2a6p |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme is homologous to a phosphatase (E.C. This enzyme catalyzes a kind of intermolecular transfer reaction, (a) 2,3-BPG (cofactor) + enzyme => 3-PGA (or 2-PGA) (product) + phosphorylated enzyme (b) 2-PGA (or 3-PGA) (substrate) + phosphorylated enzyme => 2,3-BPG (cofactor) + enzyme Accoding to the literature [14], (a) 2,3-BPG (cofactor) + enzyme => 3-PGA (or 2-PGA) (product) + phosphorylated enzyme (1) Arg59 and His181 modulate the nucleophilicity of His8, (2) His8 makes a nucleophilic attack on one of the phosphoryl groups of 2,3-BPG, (3) The transition state seems to be stabilized by Arg7, (b) 2-PGA (or 3-PGA) (substrate) + phosphorylated enzyme => 2,3-BPG (cofactor) + enzyme (4) Glu86 now acts as a general base to deprotonate and activate another hydroxyl group of substrate. (5) The activated acceptor, (6) The transition state seems to be stabilized by Arg7, |
| Created | Updated |
|---|---|
| 2005-06-14 | 2015-10-27 |