DB code: S00283

RLCP classification 3.105.250000.91 : Transfer
CATH domain 3.40.35.10 : Fructose Permease Catalytic domain
E.C. 2.7.1.69
CSA
M-CSA
MACiE

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P26380 Fructose-specific phosphotransferase enzyme IIB component
EC 2.7.1.69
PTS system fructose-specific EIIB component
EIIB-Fru
p18
NP_390584.1 (Protein)
NC_000964.3 (DNA/RNA sequence)
PF03830 (PTSIIB_sorb)
[Graphical View]

KEGG enzyme name
protein-Npi-phosphohistidine---sugar phosphotransferase
glucose permease
PTS permease
phosphotransferase, phosphohistidinoprotein-hexose
enzyme IIl4ac
gene glC proteins
gene bglC RNA formation factors
PEP-dependent phosphotransferase enzyme II
PEP-sugar phosphotransferase enzyme II
phosphoenolpyruvate-sugar phosphotransferase enzyme II
phosphohistidinoprotein-hexose phosphotransferase
phosphohistidinoprotein-hexose phosphoribosyltransferase
phosphoprotein factor-hexose phosophotransferase
protein, specific or class, gene bglC
ribonucleic acid formation factor, gene glC
sucrose phosphotransferase system II
protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase
protein-Npi-phosphohistidine:sugar Npi-phosphotransferase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P26380 PTFB_BACSU Protein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.
MAP00010 Glycolysis / Gluconeogenesis
MAP00051 Fructose and mannose metabolism
MAP00052 Galactose metabolism
MAP00053 Ascorbate and aldarate metabolism
MAP00500 Starch and sucrose metabolism
MAP00530 Aminosugars metabolism

Compound table
Substrates Products Intermediates
KEGG-id C04261 C11477 C00615 C00934
E.C.
Compound Protein N(pi)-phospho-L-histidine Sugar Protein histidine Sugar phosphate
Type aromatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ion polysaccharide aromatic ring (with nitrogen atoms),peptide/protein phosphate group/phosphate ion,polysaccharide
ChEBI
PubChem
1bleA Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P26380

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1bleA HIS 15 HIS 15(phosphorylated) phosphorylation site

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[2]
p.597, Fig.5 2

References
[1]
Resource
Comments
Medline ID
PubMed ID 9030753
Journal Eur J Biochem
Year 1997
Volume 243
Pages 306-14
Authors Seip S, Lanz R, Gutknecht R, Flukiger K, Erni B
Title The fructose transporter of Bacillus subtilis encoded by the lev operon: backbone assignment and secondary structure of the IIB(Lev) subunit.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 9551099
Journal J Mol Biol
Year 1998
Volume 276
Pages 591-602
Authors Schauder S, Nunn RS, Lanz R, Erni B, Schirmer T
Title Crystal structure of the IIB subunit of a fructose permease (IIBLev) from Bacillus subtilis.
Related PDB 1ble
Related UniProtKB P26380

Comments
The same E.C. number (2.7.1.69) appears in S00297, D00527, D00525, S00420, S00046. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, a phosphoryl group is transferred from phosphoenolpyruvate (PEP) via the PTS enzymes, EI, HPr, IIA, IIB to the tranported sugar. The enzyme here is IIB subunit of a fructose transporter (IIB-lev).
Phosphoryl transfer between the PTS components is thought to proceed by nucleophilic attack of the active histidine residue on the phospho-histidine of the target protein, resulting in inversion of configuration of the phosphoryl group.

Created Updated
2002-07-28 2009-03-04