DB code: S00283

RLCP classification 3.105.250000.91 : Transfer
CATH domain : Fructose Permease Catalytic domain

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P26380 Fructose-specific phosphotransferase enzyme IIB component
PTS system fructose-specific EIIB component
NP_390584.1 (Protein)
NC_000964.3 (DNA/RNA sequence)
PF03830 (PTSIIB_sorb)
[Graphical View]

KEGG enzyme name
protein-Npi-phosphohistidine---sugar phosphotransferase
glucose permease
PTS permease
phosphotransferase, phosphohistidinoprotein-hexose
enzyme IIl4ac
gene glC proteins
gene bglC RNA formation factors
PEP-dependent phosphotransferase enzyme II
PEP-sugar phosphotransferase enzyme II
phosphoenolpyruvate-sugar phosphotransferase enzyme II
phosphohistidinoprotein-hexose phosphotransferase
phosphohistidinoprotein-hexose phosphoribosyltransferase
phosphoprotein factor-hexose phosophotransferase
protein, specific or class, gene bglC
ribonucleic acid formation factor, gene glC
sucrose phosphotransferase system II
protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase
protein-Npi-phosphohistidine:sugar Npi-phosphotransferase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P26380 PTFB_BACSU Protein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.
MAP00010 Glycolysis / Gluconeogenesis
MAP00051 Fructose and mannose metabolism
MAP00052 Galactose metabolism
MAP00053 Ascorbate and aldarate metabolism
MAP00500 Starch and sucrose metabolism
MAP00530 Aminosugars metabolism

Compound table
Substrates Products Intermediates
KEGG-id C04261 C11477 C00615 C00934
Compound Protein N(pi)-phospho-L-histidine Sugar Protein histidine Sugar phosphate
Type aromatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ion polysaccharide aromatic ring (with nitrogen atoms),peptide/protein phosphate group/phosphate ion,polysaccharide
1bleA Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1bleA HIS 15 HIS 15(phosphorylated) phosphorylation site

References for Catalytic Mechanism
References Sections No. of steps in catalysis
p.597, Fig.5 2

Medline ID
PubMed ID 9030753
Journal Eur J Biochem
Year 1997
Volume 243
Pages 306-14
Authors Seip S, Lanz R, Gutknecht R, Flukiger K, Erni B
Title The fructose transporter of Bacillus subtilis encoded by the lev operon: backbone assignment and secondary structure of the IIB(Lev) subunit.
Related PDB
Related UniProtKB
Medline ID
PubMed ID 9551099
Journal J Mol Biol
Year 1998
Volume 276
Pages 591-602
Authors Schauder S, Nunn RS, Lanz R, Erni B, Schirmer T
Title Crystal structure of the IIB subunit of a fructose permease (IIBLev) from Bacillus subtilis.
Related PDB 1ble
Related UniProtKB P26380

The same E.C. number ( appears in S00297, D00527, D00525, S00420, S00046. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, a phosphoryl group is transferred from phosphoenolpyruvate (PEP) via the PTS enzymes, EI, HPr, IIA, IIB to the tranported sugar. The enzyme here is IIB subunit of a fructose transporter (IIB-lev).
Phosphoryl transfer between the PTS components is thought to proceed by nucleophilic attack of the active histidine residue on the phospho-histidine of the target protein, resulting in inversion of configuration of the phosphoryl group.

Created Updated
2002-07-28 2009-03-04