DB code: S00017

CATH domain 1.10.489.10 : Chloroperoxidase Catalytic domain
E.C. 1.11.1.10
CSA 2cpo
M-CSA 2cpo
MACiE M0250

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Pfam
P04963 Chloroperoxidase
EC 1.11.1.10
Chloride peroxidase
CPO
PF01328 (Peroxidase_2)
[Graphical View]

KEGG enzyme name
chloride peroxidase
chloroperoxidase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P04963 PRXC_CALFU RH + Cl(-) + H(2)O(2) = RCl + 2 H(2)O. Binds 1 heme B (iron-protoporphyrin IX) group per subunit. Binds 1 manganese ion per subunit.

KEGG Pathways
Map code Pathways E.C.

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00032 C00034 C00027 C00698 C00708 C01324 C01371 C00001 C00720 C01321 C01334
E.C.
Compound Heme Manganese H2O2 Cl- I- Br- RH H2O RBr RI RCl
Type aromatic ring (with nitrogen atoms),carboxyl group,heavy metal heavy metal others halide halide halide lipid H2O halide halide halide
ChEBI 17627
 26355
 17627
 26355
 		18291
 35154
 18291
 35154
 		16240
 16240
 		17996
 17996
 		16382
 16382
 		15858
 15858
 		15377
 15377
PubChem 23930
 23930
 		22326046
 784
 22326046
 784
 		312
 312
 		30165
 30165
 		259
 259
 		22247451
 962
 22247451
 962
1cpoA Bound:HEM Bound:_MN Unbound Unbound Unbound Unbound Unbound Bound:HOH_801 Unbound Unbound Unbound
2cpoA Bound:HEM Bound:_MN Unbound Unbound Unbound Unbound Unbound Bound:HOH_801 Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P04963

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1cpoA HIS 105;GLU 183 CYS 29(Fe binding);GLU 104;HIS 105;SER 108(Manganese binding)
2cpoA HIS 105;GLU 183 CYS 29(Fe binding);GLU 104;HIS 105;SER 108(Manganese binding)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[4]
p.1370-1374
[5]
Fig.1, Fig.2, Fig.6, Fig.8 6
[10]
Scheme 1 p.670

References
[1]
Resource
Comments
Medline ID
PubMed ID 7200984
Journal J Biol Chem
Year 1982
Volume 257
Pages 7768-9
Authors Rubin B, VanMiddlesworth J, Thomas K, Hager L
Title Crystallization and preliminary X-ray data for chloroperoxidase.
Related PDB
Related UniProtKB
[2]
Resource
Comments DETERMINATION OF HEME LIGAND
Medline ID 89066662
PubMed ID 3198598
Journal J Biol Chem
Year 1988
Volume 263
Pages 18739-43
Authors Blanke SR, Hager LP
Title Identification of the fifth axial heme ligand of chloroperoxidase.
Related PDB
Related UniProtKB P04963
[3]
Resource
Comments
Medline ID
PubMed ID 8188677
Journal J Biol Chem
Year 1994
Volume 269
Pages 13966-78
Authors Hosten CM, Sullivan AM, Palaniappan V, Fitzgerald MM, Terner J
Title Resonance Raman spectroscopy of the catalytic intermediates and derivatives of chloroperoxidase from Caldariomyces fumago.
Related PDB
Related UniProtKB
[4]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)
Medline ID 96363674
PubMed ID 8747463
Journal Structure
Year 1995
Volume 3
Pages 1367-77
Authors Sundaramoorthy M, Terner J, Poulos TL
Title The crystal structure of chloroperoxidase: a heme peroxidase--cytochrome P450 functional hybrid.
Related PDB 1cpo 2cpo
Related UniProtKB P04963
[5]
Resource
Comments
Medline ID
PubMed ID 9195874
Journal Chem Biol
Year 1997
Volume 4
Pages 367-72
Authors Wagenknecht HA, Woggon WD
Title Identification of intermediates in the catalytic cycle of chloroperoxidase.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 9671545
Journal Chem Res Toxicol
Year 1998
Volume 11
Pages 816-23
Authors Toy PH, Newcomb M, Hager LP
Title Hypersensitive radical probe studies of chloroperoxidase-catalyzed hydroxylation reactions.
Related PDB
Related UniProtKB
[7]
Resource
Comments Homologous enzyme
Medline ID
PubMed ID 9551547
Journal Structure
Year 1998
Volume 6
Pages 255-8
Authors Poulos TL, Raman CS, Li H
Title NO news is good news.
Related PDB
Related UniProtKB
[8]
Resource
Comments MUTAGENESIS OF CYS-50
Medline ID 20006248
PubMed ID 10535936
Journal Proc Natl Acad Sci U S A
Year 1999
Volume 96
Pages 12412-7
Authors Yi X, Mroczko M, Manoj KM, Wang X, Hager LP
Title Replacement of the proximal heme thiolate ligand in chloroperoxidase with a histidine residue.
Related PDB
Related UniProtKB P04963
[9]
Resource
Comments
Medline ID
PubMed ID 11460242
Journal Biotechnol Bioeng
Year 2001
Volume 72
Pages 523-9
Authors van de Velde F, Bakker M, van Rantwijk F, Sheldon RA
Title Chloroperoxidase-catalyzed enantioselective oxidations in hydrophobic organic media.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 11168405
Journal Eur J Biochem
Year 2001
Volume 268
Pages 665-72
Authors Baciocchi E, Fabbrini M, Lanzalunga O, Manduchi L, Pochetti G
Title Prochiral selectivity in H(2)O(2)-promoted oxidation of arylalkanols catalysed by chloroperoxidase. The role of the interactions between the OH group and the amino-acid residues in the enzyme active site.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 11278701
Journal J Biol Chem
Year 2001
Volume 276
Pages 17635-40
Authors Conesa A, van De Velde F, van Rantwijk F, Sheldon RA, van Den Hondel CA, Punt PJ
Title Expression of the Caldariomyces fumago chloroperoxidase in Aspergillus niger and characterization of the recombinant enzyme.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 11684191
Journal Phytochemistry
Year 2001
Volume 58
Pages 929-33
Authors Vazquez-Duhalt R, Ayala M, Marquez-Rocha FJ
Title Biocatalytic chlorination of aromatic hydrocarbons by chloroperoxidase of Caldariomyces fumago.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 12127969
Journal Biochem Biophys Res Commun
Year 2002
Volume 295
Pages 828-31
Authors Ayala M, Horjales E, Pickard MA, Vazquez-Duhalt R
Title Cross-linked crystals of chloroperoxidase.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-01-26 2009-02-26