DB code: M00178
| RLCP classification | 3.113.90000.397 : Transfer | |
|---|---|---|
| 3.1103.6090.130 : Transfer | ||
| CATH domain | 2.40.240.10 : Ribosomal Protein L25; Chain P | |
| 2.40.240.10 : Ribosomal Protein L25; Chain P | ||
| 1.10.1160.10 : Glutamyl-tRNA Synthetase; domain 2 | Catalytic domain | |
| 3.90.800.10 : Glutamyl-tRNA Synthetase; domain 3 | ||
| 3.40.50.620 : Rossmann fold | Catalytic domain | |
| E.C. | 6.1.1.18 | |
| CSA | 1euy | |
| M-CSA | 1euy | |
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.620 : Rossmann fold | S00314 S00549 S00316 S00317 S00318 S00315 T00085 T00249 D00300 M00177 T00106 T00114 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P00962 |
Glutaminyl-tRNA synthetase
|
EC
6.1.1.18
Glutamine--tRNA ligase GlnRS |
NP_415206.1
(Protein)
NC_000913.2 (DNA/RNA sequence) YP_488960.1 (Protein) NC_007779.1 (DNA/RNA sequence) |
PF00749
(tRNA-synt_1c)
PF03950 (tRNA-synt_1c_C) [Graphical View] |
| KEGG enzyme name |
|---|
|
glutamine---tRNA ligase
glutaminyl-tRNA synthetase glutaminyl-transfer RNA synthetase glutaminyl-transfer ribonucleate synthetase glutamine-tRNA synthetase glutamine translase glutamate-tRNA ligase glutaminyl ribonucleic acid GlnRS |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P00962 | SYQ_ECOLI | ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). | Monomer. | Cytoplasm. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00251 | Glutamate metabolism | |
| MAP00970 | Aminoacyl-tRNA biosynthesis |
| Compound table | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||||
| KEGG-id | C00305 | C00002 | C00064 | C01640 | C00020 | C00013 | C02282 | ||||||
| E.C. | |||||||||||||
| Compound | Magnesium | ATP | L-Glutamine | tRNA(Gln) | AMP | Pyrophosphate | L-Glutaminyl-tRNA(Gln) | Glutaminyl-adenylate | |||||
| Type | divalent metal (Ca2+, Mg2+) | amine group,nucleotide | amino acids,amide group | nucleic acids | amine group,nucleotide | phosphate group/phosphate ion | amino acids,amide group,nucleic acids | ||||||
| ChEBI |
18420 18420 |
15422 15422 |
18050 58359 18050 58359 |
16027 16027 |
29888 29888 |
||||||||
| PubChem |
888 888 |
5957 5957 |
5961 6992086 5961 6992086 |
6083 6083 |
1023 21961011 1023 21961011 |
||||||||
| 1euqA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1euyA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1exdA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gsgP01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gtrA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gtsA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1nylA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1o0bA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1o0cA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qrsA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qrtA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qruA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qtqA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1zjwA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1euqA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1euyA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1exdA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gsgP02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gtrA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gtsA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1nylA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1o0bA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1o0cA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qrsA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qrtA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qruA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qtqA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1zjwA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1euqA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1euyA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1exdA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gsgP03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gtrA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gtsA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1nylA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1o0bA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1o0cA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qrsA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qrtA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qruA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qtqA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1zjwA03 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1euqA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1euyA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1exdA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gsgP04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gtrA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1gtsA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1nylA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1o0bA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1o0cA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qrsA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qrtA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qruA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1qtqA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1zjwA04 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1euqA05 |
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Unbound | Unbound | Unbound | Bound:G-C-C-A_976 (chain B:3'-terminus) | Unbound | Unbound | Unbound | Intermediate-analogue:QSI |
| 1euyA05 |
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Unbound | Unbound | Unbound | Bound:G-C-C-A_976 (chain B:3'-terminus) | Unbound | Unbound | Unbound | Intermediate-analogue:QSI |
| 1exdA05 |
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Unbound | Unbound | Unbound | Bound:G-C-C-A_976 (chain B:3'-terminus) | Analogue:__A | Unbound | Unbound | Unbound |
| 1gsgP05 |
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Unbound | Unbound | Unbound | Bound:G-C-C-A_76 (chain T:3'-terminus) | Unbound | Unbound | Unbound | Unbound |
| 1gtrA05 |
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Unbound | Bound:ATP | Unbound | Bound:G-C-C-A_76 (chain B:3'-terminus) | Unbound | Unbound | Unbound | Unbound |
| 1gtsA05 |
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Unbound | Unbound | Unbound | Bound:G-C-C-A_76 (chain B:3'-terminus) | Bound:AMP | Unbound | Unbound | Unbound |
| 1nylA05 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound |
| 1o0bA05 |
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Unbound | Unbound | Bound:GLN | Bound:G-C-C-A_976 (chain B:3'-terminus) | Analogue:AMP | Analogue:SO4_1394 | Unbound | Unbound |
| 1o0cA05 |
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Unbound | Unbound | Analogue:GLU | Bound:G-C-C-A_976 (chain B:3'-terminus) | Bound:AMP | Analogue:SO4_1394 | Unbound | Unbound |
| 1qrsA05 |
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Unbound | Bound:ATP | Unbound | Bound:G-C-C-A_76 (chain B:3'-terminus) | Unbound | Unbound | Unbound | Unbound |
| 1qrtA05 |
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Unbound | Bound:ATP | Unbound | Bound:G-C-C-A_76 (chain B:3'-terminus) | Unbound | Unbound | Unbound | Unbound |
| 1qruA05 |
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Unbound | Bound:ATP | Unbound | Bound:G-C-C-A_76 (chain B:3'-terminus) | Unbound | Unbound | Unbound | Unbound |
| 1qtqA05 |
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Unbound | Unbound | Unbound | Bound:G-C-C-A_976 (chain B:3'-terminus) | Unbound | Analogue:SO4_1394 | Unbound | Intermediate-analogue:QSI |
| 1zjwA05 |
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Unbound | Unbound | Bound:GLN | Analogue:G-C-C-A_976 (chain B:3'-terminus) | Bound:AMP | Analogue:SO4_1394 | Unbound | Unbound |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| literature [33] | ||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[10]
|
Fig.13, p.8768-8771 | |
|
[33]
|
Fig.9, p.446-447 | 2 |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2459391 |
| Journal | J Mol Biol |
| Year | 1988 |
| Volume | 202 |
| Pages | 121-6 |
| Authors | Perona JJ, Swanson R, Steitz TA, Soll D |
| Title | Overproduction and purification of Escherichia coli tRNA(2Gln) and its use in crystallization of the glutaminyl-tRNA synthetase-tRNA(Gln) complex. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2686030 |
| Journal | Science |
| Year | 1989 |
| Volume | 246 |
| Pages | 1152-4 |
| Authors | Perona JJ, Swanson RN, Rould MA, Steitz TA, Soll D |
| Title |
Structural basis for misaminoacylation by mutant E. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS). |
| Medline ID | 90069584 |
| PubMed ID | 2479982 |
| Journal | Science |
| Year | 1989 |
| Volume | 246 |
| Pages | 1135-42 |
| Authors | Rould MA, Perona JJ, Soll D, Steitz TA |
| Title |
Structure of E. |
| Related PDB | 1gsg |
| Related UniProtKB | P00962 |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2194108 |
| Journal | Mol Biol Rep |
| Year | 1990 |
| Volume | 14 |
| Pages | 213-4 |
| Authors | Steitz TA, Rould MA, Perona JJ |
| Title | Structural basis of tRNA discrimination as derived from the high resolution crystal structure of glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1915346 |
| Journal | Eur J Biochem |
| Year | 1991 |
| Volume | 200 |
| Pages | 739-45 |
| Authors | Bhattacharyya T, Bhattacharyya A, Roy S |
| Title | A fluorescence spectroscopic study of glutaminyl-tRNA synthetase from Escherichia coli and its implications for the enzyme mechanism. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1857423 |
| Journal | Nature |
| Year | 1991 |
| Volume | 352 |
| Pages | 258-60 |
| Authors | Jahn M, Rogers MJ, Soll D |
| Title |
Anticodon and acceptor stem nucleotides in tRNA(Gln) are major recognition elements for E. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). |
| Medline ID | 91312443 |
| PubMed ID | 1857417 |
| Journal | Nature |
| Year | 1991 |
| Volume | 352 |
| Pages | 213-8 |
| Authors | Rould MA, Perona JJ, Steitz TA |
| Title | Structural basis of anticodon loop recognition by glutaminyl-tRNA synthetase. |
| Related PDB | 1gtr |
| Related UniProtKB | P00962 |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2011598 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1991 |
| Volume | 88 |
| Pages | 2903-7 |
| Authors | Perona JJ, Rould MA, Steitz TA, Risler JL, Zelwer C, Brunie S |
| Title | Structural similarities in glutaminyl- and methionyl-tRNA synthetases suggest a common overall orientation of tRNA binding. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8369295 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 9268-73 |
| Authors | Bhattacharyya T, Roy S |
| Title | A fluorescence spectroscopic study of substrate-induced conformational changes in glutaminyl-tRNA synthetase. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8364025 |
| Journal | Biochemistry |
| Year | 1993 |
| Volume | 32 |
| Pages | 8758-71 |
| Authors | Perona JJ, Rould MA, Steitz TA |
| Title | Structural basis for transfer RNA aminoacylation by Escherichia coli glutaminyl-tRNA synthetase. |
| Related PDB | 1gts |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7515283 |
| Journal | Biochimie |
| Year | 1993 |
| Volume | 75 |
| Pages | 1051-60 |
| Authors | Dyson MR, Mandal N, RajBhandary UL |
| Title | Relationship between the structure and function of Escherichia coli initiator tRNA. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8199248 |
| Journal | Biochimie |
| Year | 1993 |
| Volume | 75 |
| Pages | 1125-36 |
| Authors | McClain WH, Schneider J, Gabriel K |
| Title | Association of tRNA(Gln) acceptor identity with phosphate-sugar backbone interactions observed in the crystal structure of the Escherichia coli glutaminyl-tRNA synthetase-tRNA(Gln) complex. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8199243 |
| Journal | Biochimie |
| Year | 1993 |
| Volume | 75 |
| Pages | 1083-90 |
| Authors | Rogers MJ, Weygand-Durasevic I, Schwob E, Sherman JM, Rogers KC, Adachi T, Inokuchi H, Soll D |
| Title | Selectivity and specificity in the recognition of tRNA by E coli glutaminyl-tRNA synthetase. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7505222 |
| Journal | EMBO J |
| Year | 1993 |
| Volume | 12 |
| Pages | 5201-8 |
| Authors | Schwob E, Soll D |
| Title | Selection of a 'minimal' glutaminyl-tRNA synthetase and the evolution of class I synthetases. |
| Related PDB | |
| Related UniProtKB | |
| [15] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8360919 |
| Journal | J Mol Evol |
| Year | 1993 |
| Volume | 37 |
| Pages | 5-10 |
| Authors | Di Giulio M |
| Title | Origin of glutaminyl-tRNA synthetase: an example of palimpsest? |
| Related PDB | |
| Related UniProtKB | |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7680483 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1993 |
| Volume | 90 |
| Pages | 2010-4 |
| Authors | Weygand-Durasevic I, Schwob E, Soll D |
| Title | Acceptor end binding domain interactions ensure correct aminoacylation of transfer RNA. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8011621 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 7560-7 |
| Authors | Arnez JG, Steitz TA |
| Title | Crystal structure of unmodified tRNA(Gln) complexed with glutaminyl-tRNA synthetase and ATP suggests a possible role for pseudo-uridines in stabilization of RNA structure. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8027995 |
| Journal | J Mol Biol |
| Year | 1994 |
| Volume | 240 |
| Pages | 111-8 |
| Authors | Weygand-Durasevic I, Rogers MJ, Soll D |
| Title | Connecting anticodon recognition with the active site of Escherichia coli glutaminyl-tRNA synthetase. |
| Related PDB | |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7506418 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1994 |
| Volume | 91 |
| Pages | 291-5 |
| Authors | Rogers MJ, Adachi T, Inokuchi H, Soll D |
| Title | Functional communication in the recognition of tRNA by Escherichia coli glutaminyl-tRNA synthetase. |
| Related PDB | |
| Related UniProtKB | |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8643392 |
| Journal | Nucleic Acids Symp Ser |
| Year | 1995 |
| Volume | (33) |
| Pages | 40-2 |
| Authors | Ibba M, Thomann HU, Hong KW, Sherman JM, Weygand-Durasevic I, Sever S, Stange-Thomann N, Praetorius M, Soll D |
| Title | Substrate selection by aminoacyl-tRNA synthetases. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8942633 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 14725-33 |
| Authors | Arnez JG, Steitz TA |
| Title | Crystal structures of three misacylating mutants of Escherichia coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP. |
| Related PDB | 1qrs 1qrt 1qru |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8555233 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 601-7 |
| Authors | Sherman JM, Soll D |
| Title | Aminoacyl-tRNA synthetases optimize both cognate tRNA recognition and discrimination against noncognate tRNAs. |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8601833 |
| Journal | J Mol Biol |
| Year | 1996 |
| Volume | 256 |
| Pages | 818-28 |
| Authors | Sherman JM, Thomann HU, Soll D |
| Title | Functional connectivity between tRNA binding domains in glutaminyl-tRNA synthetase. |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8917315 |
| Journal | Mol Gen Genet |
| Year | 1996 |
| Volume | 252 |
| Pages | 717-22 |
| Authors | Kitabatake M, Ibba M, Hong KW, Soll D, Inokuchi H |
| Title | Genetic analysis of functional connectivity between substrate recognition domains of Escherichia coli glutaminyl-tRNA synthetase. |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8692925 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 1996 |
| Volume | 93 |
| Pages | 6953-8 |
| Authors | Ibba M, Hong KW, Sherman JM, Sever S, Soll D |
| Title | Interactions between tRNA identity nucleotides and their recognition sites in glutaminyl-tRNA synthetase determine the cognate amino acid affinity of the enzyme. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9372179 |
| Journal | Biol Chem |
| Year | 1997 |
| Volume | 378 |
| Pages | 1103-17 |
| Authors | Freist W, Gauss DH, Ibba M, Soll D |
| Title | Glutaminyl-tRNA synthetase. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9185564 |
| Journal | Nucleic Acids Res |
| Year | 1997 |
| Volume | 25 |
| Pages | 2562-5 |
| Authors | Lustig B, Arora S, Jernigan RL |
| Title | RNA base-amino acid interaction strengths derived from structures and sequences. |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9657697 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 9836-42 |
| Authors | Liu J, Ibba M, Hong KW, Soll D |
| Title | The terminal adenosine of tRNA(Gln) mediates tRNA-dependent amino acid recognition by glutaminyl-tRNA synthetase. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9799245 |
| Journal | EMBO J |
| Year | 1998 |
| Volume | 17 |
| Pages | 6377-84 |
| Authors | Stoldt M, Wohnert J, Gorlach M, Brown LR |
| Title | The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases. |
| Related PDB | |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9746349 |
| Journal | Eur J Biochem |
| Year | 1998 |
| Volume | 256 |
| Pages | 80-7 |
| Authors | Siatecka M, Rozek M, Barciszewski J, Mirande M |
| Title | Modular evolution of the Glx-tRNA synthetase family--rooting of the evolutionary tree between the bacteria and archaea/eukarya branches. |
| Related PDB | |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9738468 |
| Journal | FEBS Lett |
| Year | 1998 |
| Volume | 434 |
| Pages | 149-54 |
| Authors | Hong KW, Ibba M, Soll D |
| Title | Retracing the evolution of amino acid specificity in glutaminyl-tRNA synthetase. |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9568911 |
| Journal | Protein Sci |
| Year | 1998 |
| Volume | 7 |
| Pages | 1046-51 |
| Authors | Mandal AK, Bhattacharyya A, Bhattacharyya S, Bhattacharyya T, Roy S |
| Title | A cognate tRNA specific conformational change in glutaminyl-tRNA synthetase and its implication for specificity. |
| Related PDB | |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). |
| Medline ID | 98230743 |
| PubMed ID | 9562563 |
| Journal | Structure |
| Year | 1998 |
| Volume | 6 |
| Pages | 439-49 |
| Authors | Rath VL, Silvian LF, Beijer B, Sproat BS, Steitz TA |
| Title | How glutaminyl-tRNA synthetase selects glutamine. |
| Related PDB | 1qtq |
| Related UniProtKB | P00962 |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10801842 |
| Journal | J Biol Chem |
| Year | 2000 |
| Volume | 275 |
| Pages | 21768-72 |
| Authors | Kim T, Park SG, Kim JE, Seol W, Ko YG, Kim S |
| Title | Catalytic peptide of human glutaminyl-tRNA synthetase is essential for its assembly to the aminoacyl-tRNA synthetase complex. |
| Related PDB | |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS). |
| Medline ID | 20318776 |
| PubMed ID | 10860750 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 299 |
| Pages | 431-46 |
| Authors | Sherlin LD, Bullock TL, Newberry KJ, Lipman RS, Hou YM, Beijer B, Sproat BS, Perona JJ |
| Title | Influence of transfer RNA tertiary structure on aminoacylation efficiency by glutaminyl and cysteinyl-tRNA synthetases. |
| Related PDB | 1euq 1euy |
| Related UniProtKB | P00962 |
| [36] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10881199 |
| Journal | Nat Struct Biol |
| Year | 2000 |
| Volume | 7 |
| Pages | 497-504 |
| Authors | Bullock TL, Sherlin LD, Perona JJ |
| Title | Tertiary core rearrangements in a tight binding transfer RNA aptamer. |
| Related PDB | 1exd |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11224555 |
| Journal | Nat Struct Biol |
| Year | 2001 |
| Volume | 8 |
| Pages | 189-91 |
| Authors | Francklyn CS |
| Title | Charging two for the price of one. |
| Related PDB | |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11724963 |
| Journal | Proc Natl Acad Sci U S A |
| Year | 2001 |
| Volume | 98 |
| Pages | 14244-9 |
| Authors | Patzelt H, Rudiger S, Brehmer D, Kramer G, Vorderwulbecke S, Schaffitzel E, Waitz A, Hesterkamp T, Dong L, Schneider-Mergener J, Bukau B, Deuerling E |
| Title | Binding specificity of Escherichia coli trigger factor. |
| Related PDB | |
| Related UniProtKB | |
| [39] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12691748 |
| Journal | J Mol Biol |
| Year | 2003 |
| Volume | 328 |
| Pages | 395-408 |
| Authors | Bullock TL, Uter N, Nissan TA, Perona JJ |
| Title | Amino acid discrimination by a class I aminoacyl-tRNA synthetase specified by negative determinants. |
| Related PDB | 1o0b 1o0c |
| Related UniProtKB | |
| [40] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 12737824 |
| Journal | Structure (Camb) |
| Year | 2003 |
| Volume | 11 |
| Pages | 591-603 |
| Authors | Sherlin LD, Perona JJ |
| Title | tRNA-dependent active site assembly in a class I aminoacyl-tRNA synthetase. |
| Related PDB | 1nyl |
| Related UniProtKB | |
| [41] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15845536 |
| Journal | J Biol Chem |
| Year | 2005 |
| Volume | 280 |
| Pages | 23978-86 |
| Authors | Gruic-Sovulj I, Uter N, Bullock T, Perona JJ |
| Title | tRNA-dependent aminoacyl-adenylate hydrolysis by a nonediting class I aminoacyl-tRNA synthetase. |
| Related PDB | 1zjw |
| Related UniProtKB | |
| Comments |
|---|
|
According to literature [26], This enzyme catalyzes two successive transfer reactions, (A) Transfer of adenylate from ATP to carboxyl oxygen of glutamine substrate: (A1) The carboxylate oxygen makes a nucleophilic attack on the transferred group, (A2) The transferred group is stabilized by mainchain amide of Glu34, (A3) This reaction gives glutaminyl-adenylate intermediate and pyrophosphate. (B) Transfer of acyl group from glutaminyl-adenylate intermediate to 2'-OH of 3'-terminus of tRNA: (B1) Glu34 acts as a general base to deprotonate the acceptor group, (B2) The activated acceptor group, (B3) The transferred group, (B4) Finally, |
| Created | Updated |
|---|---|
| 2004-09-14 | 2009-02-26 |