DB code: M00140
| CATH domain | 3.40.420.10 : Ricin (A subunit); domain 1 | Catalytic domain |
|---|---|---|
| 4.10.470.10 : Ricin (A Subunit), domain 2 | Catalytic domain | |
| 2.40.50.70 : OB fold (Dihydrolipoamide Acetyltransferase, E2P) | ||
| 2.40.50.70 : OB fold (Dihydrolipoamide Acetyltransferase, E2P) | ||
| 2.40.50.70 : OB fold (Dihydrolipoamide Acetyltransferase, E2P) | ||
| 2.40.50.70 : OB fold (Dihydrolipoamide Acetyltransferase, E2P) | ||
| 2.40.50.70 : OB fold (Dihydrolipoamide Acetyltransferase, E2P) | ||
| E.C. | 3.2.2.22 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.420.10 : Ricin (A subunit); domain 1 | D00847 |
| 4.10.470.10 : Ricin (A Subunit), domain 2 | D00847 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Pfam |
|---|---|---|---|
| P10149 |
Shiga-like toxin 1 subunit A
|
SLT-1 A subunit
SLT-1a SLT-Ia EC 3.2.2.22 Verotoxin 1 subunit A Verocytotoxin 1 subunit A rRNA N-glycosidase 1 |
PF00161
(RIP)
[Graphical View] |
| P69178 |
Shiga-like toxin 1 subunit B
|
SLT-1 B subunit
SLT-1b SLT-Ib Verocytotoxin 1 subunit B Verotoxin 1 subunit B |
PF02258
(SLT_beta)
[Graphical View] |
| KEGG enzyme name |
|---|
|
rRNA N-glycosylase
ribosomal ribonucleate N-glycosidase nigrin b RNA N-glycosidase rRNA N-glycosidase ricin momorcochin-S Mirabilis antiviral protein momorcochin-S gelonin saporins |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P10149 | STXA_BPH30 | Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. | Shiga-like toxin contains a single subunit A and five copies of subunit B. | Secreted. | |
| P69178 | STXB_BPH30 | Shiga-like toxin contains a single subunit A and five copies of subunit B. | Secreted. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|
| Compound table | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00240 | C00001 | L00063 | C00147 | ||||||
| E.C. | ||||||||||
| Compound | rRNA | H2O | Deadenylated rRNA | Adenine | ||||||
| Type | nucleic acids | H2O | nucleic acids | amine group,aromatic ring (with nitrogen atoms) | ||||||
| ChEBI |
15377 15377 |
16708 16708 |
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| PubChem |
22247451 962 22247451 962 |
190 190 |
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| 1dm0A01 |
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Unbound | Unbound | Unbound | ||
| 1dm0L01 |
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Unbound | Unbound | Unbound | ||
| 1dm0A02 |
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Unbound | Unbound | Unbound | ||
| 1dm0L02 |
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Unbound | Unbound | Unbound | ||
| 1dm0B |
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Unbound | Unbound | Unbound | ||
| 1dm0C |
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Unbound | Unbound | Unbound | ||
| 1dm0D |
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Unbound | Unbound | Unbound | ||
| 1dm0E |
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Unbound | Unbound | Unbound | ||
| 1dm0F |
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Unbound | Unbound | Unbound | ||
| 1dm0G |
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Unbound | Unbound | Unbound | ||
| 1dm0H |
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Unbound | Unbound | Unbound | ||
| 1dm0I |
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Unbound | Unbound | Unbound | ||
| 1dm0J |
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Unbound | Unbound | Unbound | ||
| 1dm0K |
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Unbound | Unbound | Unbound | ||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P10149 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1dm0A01 |
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GLU 167 | ||||
| 1dm0L01 |
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GLU 167 | ||||
| 1dm0A02 |
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ARG 170 | ||||
| 1dm0L02 |
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ARG 170 | ||||
| 1dm0B |
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| 1dm0C |
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| 1dm0D |
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| 1dm0E |
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| 1dm0F |
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| 1dm0G |
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| 1dm0H |
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| 1dm0I |
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| 1dm0J |
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| 1dm0K |
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| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1717316 |
| Journal | FEBS Lett |
| Year | 1991 |
| Volume | 290 |
| Pages | 65-8 |
| Authors | Hartley MR, Legname G, Osborn R, Chen Z, Lord JM |
| Title | Single-chain ribosome inactivating proteins from plants depurinate Escherichia coli 23S ribosomal RNA. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1619659 |
| Journal | J Mol Biol |
| Year | 1992 |
| Volume | 226 |
| Pages | 281-3 |
| Authors | Miyano M, Appelt K, Arita M, Habuka N, Kataoka J, Ago H, Tsuge H, Noma M, Ashford V, Xuong N |
| Title | Crystallization and preliminary X-ray crystallographic analysis of Mirabilis antiviral protein. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | X-ray Diffraction |
| Medline ID | |
| PubMed ID | 8345529 |
| Journal | J Mol Biol |
| Year | 1993 |
| Volume | 232 |
| Pages | 704-6 |
| Authors | Kozlov YV, Chernaia MM, Fraser ME, James MN |
| Title | Purification and crystallization of Shiga toxin from Shigella dysenteriae. |
| Related PDB | 1dm0 |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8218413 |
| Journal | Biochim Biophys Acta |
| Year | 1993 |
| Volume | 1216 |
| Pages | 31-42 |
| Authors | Ferreras JM, Barbieri L, Girbes T, Battelli MG, Rojo MA, Arias FJ, Rocher MA, Soriano F, Mendez E, Stirpe F |
| Title |
Distribution and properties of major ribosome-inactivating proteins (28 S rRNA N-glycosidases) of the plant Saponaria officinalis L. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | X-ray Diffraction |
| Medline ID | |
| PubMed ID | 7656009 |
| Journal | Nat Struct Biol |
| Year | 1994 |
| Volume | 1 |
| Pages | 59-64 |
| Authors | Fraser ME, Chernaia MM, Kozlov YV, James MN |
| Title | Crystal structure of the holotoxin from Shigella dysenteriae at 2.5 A resolution. |
| Related PDB | 1dm0 |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8912688 |
| Journal | Biochem J |
| Year | 1996 |
| Volume | 319 |
| Pages | 507-13 |
| Authors | Barbieri L, Valbonesi P, Gorini P, Pession A, Stirpe F |
| Title |
Polynucleotide: adenosine glycosidase activity of saporin-L1: effect on DNA, |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9398319 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 15865-72 |
| Authors | Menikh A, Saleh MT, Gariepy J, Boggs JM |
| Title |
Orientation in lipid bilayers of a synthetic peptide representing the C-terminus of the A1 domain of shiga toxin. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9367352 |
| Journal | FASEB J |
| Year | 1997 |
| Volume | 11 |
| Pages | 1169-76 |
| Authors | Fabbrini MS, Carpani D, Bello-Rivero I, Soria MR |
| Title | The amino-terminal fragment of human urokinase directs a recombinant chimeric toxin to target cells: internalization is toxin mediated. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9761860 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1998 |
| Volume | 54 |
| Pages | 636-8 |
| Authors | Savino C, Federici L, Brancaccio A, Ippoliti R, Lendaro E, Tsernoglou D |
| Title |
Crystallization and preliminary X-ray study of saporin, |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9989237 |
| Journal | Biochim Biophys Acta |
| Year | 1999 |
| Volume | 1429 |
| Pages | 506-11 |
| Authors | Kumar M, Dattagupta S, Kannan KK, Hosur MV |
| Title |
Purification, |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10745075 |
| Journal | FEBS Lett |
| Year | 2000 |
| Volume | 470 |
| Pages | 239-43 |
| Authors | Savino C, Federici L, Ippoliti R, Lendaro E, Tsernoglou D |
| Title | The crystal structure of saporin SO6 from Saponaria officinalis and its interaction with the ribosome. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme belongs to ribosome-inactivating protein (RIP) family (see [5]).
|
| Created | Updated |
|---|---|
| 2004-03-19 | 2011-11-01 |