DB code: D00527

RLCP classification	3.105.230000.86 : Transfer
CATH domain	3.40.50.510 : Rossmann fold	Catalytic domain
CATH domain	3.40.35.- : Fructose Permease
E.C.	2.7.1.- 2.7.1.69
CSA
M-CSA
MACiE

CATH domain	Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB	Protein name	Synonyms	Includes	RefSeq	Pfam
P69797	PTS system mannose-specific EIIAB component	EIIAB-Man	Mannose-specific phosphotransferase enzyme IIA component EC 2.7.1.- PTS system mannose-specific EIIA component EIII-Man Mannose-specific phosphotransferase enzyme IIB component EC 2.7.1.69 PTS system mannose-specific EIIB component	NP_416331.1 (Protein) NC_000913.2 (DNA/RNA sequence) YP_490078.1 (Protein) NC_007779.1 (DNA/RNA sequence)	PF03610 (EIIA-man) PF03830 (PTSIIB_sorb) [Graphical View]

KEGG enzyme name
protein-Npi-phosphohistidine---sugar phosphotransferase (EC 2.7.1.69 ) glucose permease (EC 2.7.1.69 ) PTS permease (EC 2.7.1.69 ) phosphotransferase, phosphohistidinoprotein-hexose (EC 2.7.1.69 ) enzyme IIl4ac (EC 2.7.1.69 ) gene glC proteins (EC 2.7.1.69 ) gene bglC RNA formation factors (EC 2.7.1.69 ) PEP-dependent phosphotransferase enzyme II (EC 2.7.1.69 ) PEP-sugar phosphotransferase enzyme II (EC 2.7.1.69 ) phosphoenolpyruvate-sugar phosphotransferase enzyme II (EC 2.7.1.69 ) phosphohistidinoprotein-hexose phosphotransferase (EC 2.7.1.69 ) phosphohistidinoprotein-hexose phosphoribosyltransferase (EC 2.7.1.69 ) phosphoprotein factor-hexose phosophotransferase (EC 2.7.1.69 ) protein, specific or class, gene bglC (EC 2.7.1.69 ) ribonucleic acid formation factor, gene glC (EC 2.7.1.69 ) sucrose phosphotransferase system II (EC 2.7.1.69 ) protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase (EC 2.7.1.69 ) protein-Npi-phosphohistidine:sugar Npi-phosphotransferase (EC 2.7.1.69 )

KEGG enzyme name

protein-Npi-phosphohistidine---sugar phosphotransferase
(EC 2.7.1.69 )
glucose permease
(EC 2.7.1.69 )
PTS permease
(EC 2.7.1.69 )
phosphotransferase, phosphohistidinoprotein-hexose
(EC 2.7.1.69 )
enzyme IIl4ac
(EC 2.7.1.69 )
gene glC proteins
(EC 2.7.1.69 )
gene bglC RNA formation factors
(EC 2.7.1.69 )
PEP-dependent phosphotransferase enzyme II
(EC 2.7.1.69 )
PEP-sugar phosphotransferase enzyme II
(EC 2.7.1.69 )
phosphoenolpyruvate-sugar phosphotransferase enzyme II
(EC 2.7.1.69 )
phosphohistidinoprotein-hexose phosphotransferase
(EC 2.7.1.69 )
phosphohistidinoprotein-hexose phosphoribosyltransferase
(EC 2.7.1.69 )
phosphoprotein factor-hexose phosophotransferase
(EC 2.7.1.69 )
protein, specific or class, gene bglC
(EC 2.7.1.69 )
ribonucleic acid formation factor, gene glC
(EC 2.7.1.69 )
sucrose phosphotransferase system II
(EC 2.7.1.69 )
protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase
(EC 2.7.1.69 )
protein-Npi-phosphohistidine:sugar Npi-phosphotransferase
(EC 2.7.1.69 )

UniprotKB: Accession Number	Entry name	Activity	Subunit	Subcellular location	Cofactor
P69797	PTNAB_ECOLI	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L- histidine/cysteine. Protein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate.		Cytoplasm. Cell inner membrane, Peripheral membrane protein.

KEGG Pathways
Map code	Pathways	E.C.
MAP00010	Glycolysis / Gluconeogenesis	2.7.1.69
MAP00051	Fructose and mannose metabolism	2.7.1.69
MAP00052	Galactose metabolism	2.7.1.69
MAP00053	Ascorbate and aldarate metabolism	2.7.1.69
MAP00500	Starch and sucrose metabolism	2.7.1.69
MAP00530	Aminosugars metabolism	2.7.1.69

Compound table
	Substrates			Products			Intermediates
KEGG-id	C02743	C04261	C11477	L00031	C00615	C00934
E.C.	2.7.1.-	2.7.1.- 2.7.1.69	2.7.1.69	2.7.1.-	2.7.1.- 2.7.1.69	2.7.1.69
Compound	Protein cysteine	Protein N(pi)-phospho-L-histidine	Sugar	Protein S-phosphoryl-cysteine	Protein histidine	Sugar phosphate
Type	peptide/protein,sulfhydryl group	aromatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ion	polysaccharide	peptide/protein,phosphate group/phosphate ion,sulfide group	aromatic ring (with nitrogen atoms),peptide/protein	phosphate group/phosphate ion,polysaccharide
ChEBI
PubChem

1pdoA	Unbound	Unbound	Unbound	Unbound	Unbound	Unbound

Reference for Active-site residues
resource	references	E.C.
literature [2]

Active-site residues
PDB	Catalytic residues	Cofactor-binding residues	Modified residues	Main-chain involved in catalysis	Comment

1pdoA	HIS 10;ASP 67		HIS 10(phosphorylated)

References for Catalytic Mechanism
References	Sections	No. of steps in catalysis
[2]	p.508-509

References
[1]
Resource
Comments
Medline ID
PubMed ID	8131846
Journal	FEBS Lett
Year	1994
Volume	340
Pages	202-6
Authors	Markovic-Housley Z, Balbach J, Stolz B, Genovesio-Taverne JC
Title	Predicted topology of the N-terminal domain of the hydrophilic subunit of the mannose transporter of Escherichia coli.
Related PDB
Related UniProtKB	P69797
[2]
Resource
Comments
Medline ID
PubMed ID	8676384
Journal	J Mol Biol
Year	1996
Volume	259
Pages	502-11
Authors	Nunn RS, Markovic-Housley Z, Genovesio-Taverne JC, Flukiger K, Rizkallah PJ, Jansonius JN, Schirmer T, Erni B
Title	Structure of the IIA domain of the mannose transporter from Escherichia coli at 1.7 angstroms resolution.
Related PDB	1pdo
Related UniProtKB	P69797
[3]
Resource
Comments
Medline ID
PubMed ID	9074635
Journal	FEBS Lett
Year	1997
Volume	404
Pages	45-50
Authors	Gschwind RM, Gemmecker G, Leutner M, Kessler H, Gutknecht R, Lanz R, Flukiger K, Erni B
Title	Secondary structure of the IIB domain of the Escherichia coli mannose transporter, a new fold in the class of alpha/beta twisted open-sheet structures.
Related PDB
Related UniProtKB	P69797

Comments
The same E.C. number (2.7.1.69) appears in S00297, D00525, S00283, S00420. All of them are enzymes in PTS system. In the phosphotransferase (PTS) system, a phosphoryl group is transferred from phosphoenolpyruvate (PEP) via the PTS enzymes, EI, HPr, IIA, IIB to the tranported sugar. This enzyme is composed of IIA domain and IIB domain of a mannose transporter (IIAB-mannose). In the IIA domain, His10 is hydrogen-bonded to a buried Asp67 that is not charge compensated. The carboxylate group of Asp67 acts as a general base and increases the basic strength of the nearby imidazole ring of His10. The nucleophilic attack of the exposed imidazole NE atom onto the phosphorus of phospho-HPr would thus be facilitated.

Comments

The same E.C. number (2.7.1.69) appears in S00297, D00525, S00283, S00420. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, a phosphoryl group is transferred from phosphoenolpyruvate (PEP) via the PTS enzymes, EI, HPr, IIA, IIB to the tranported sugar.
This enzyme is composed of IIA domain and IIB domain of a mannose transporter (IIAB-mannose).
In the IIA domain, His10 is hydrogen-bonded to a buried Asp67 that is not charge compensated. The carboxylate group of Asp67 acts as a general base and increases the basic strength of the nearby imidazole ring of His10. The nucleophilic attack of the exposed imidazole NE atom onto the phosphorus of phospho-HPr would thus be facilitated.

Created	Updated
2002-07-27	2009-02-26