DB code: D00527

RLCP classification 3.105.230000.86 : Transfer
CATH domain : Rossmann fold Catalytic domain
3.40.35.- : Fructose Permease
E.C. 2.7.1.-

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Includes RefSeq Pfam
P69797 PTS system mannose-specific EIIAB component
Mannose-specific phosphotransferase enzyme IIA component
EC 2.7.1.-
PTS system mannose-specific EIIA component EIII-Man
Mannose-specific phosphotransferase enzyme IIB component
PTS system mannose-specific EIIB component
NP_416331.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_490078.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF03610 (EIIA-man)
PF03830 (PTSIIB_sorb)
[Graphical View]

KEGG enzyme name
protein-Npi-phosphohistidine---sugar phosphotransferase
(EC )
glucose permease
(EC )
PTS permease
(EC )
phosphotransferase, phosphohistidinoprotein-hexose
(EC )
enzyme IIl4ac
(EC )
gene glC proteins
(EC )
gene bglC RNA formation factors
(EC )
PEP-dependent phosphotransferase enzyme II
(EC )
PEP-sugar phosphotransferase enzyme II
(EC )
phosphoenolpyruvate-sugar phosphotransferase enzyme II
(EC )
phosphohistidinoprotein-hexose phosphotransferase
(EC )
phosphohistidinoprotein-hexose phosphoribosyltransferase
(EC )
phosphoprotein factor-hexose phosophotransferase
(EC )
protein, specific or class, gene bglC
(EC )
ribonucleic acid formation factor, gene glC
(EC )
sucrose phosphotransferase system II
(EC )
protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase
(EC )
protein-Npi-phosphohistidine:sugar Npi-phosphotransferase
(EC )

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P69797 PTNAB_ECOLI Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L- histidine/cysteine. Protein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate. Cytoplasm. Cell inner membrane, Peripheral membrane protein.

KEGG Pathways
Map code Pathways E.C.
MAP00010 Glycolysis / Gluconeogenesis
MAP00051 Fructose and mannose metabolism
MAP00052 Galactose metabolism
MAP00053 Ascorbate and aldarate metabolism
MAP00500 Starch and sucrose metabolism
MAP00530 Aminosugars metabolism

Compound table
Substrates Products Intermediates
KEGG-id C02743 C04261 C11477 L00031 C00615 C00934
E.C. 2.7.1.-
Compound Protein cysteine Protein N(pi)-phospho-L-histidine Sugar Protein S-phosphoryl-cysteine Protein histidine Sugar phosphate
Type peptide/protein,sulfhydryl group aromatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ion polysaccharide peptide/protein,phosphate group/phosphate ion,sulfide group aromatic ring (with nitrogen atoms),peptide/protein phosphate group/phosphate ion,polysaccharide
1pdoA Unbound Unbound Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
literature [2]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1pdoA HIS 10;ASP 67 HIS 10(phosphorylated)

References for Catalytic Mechanism
References Sections No. of steps in catalysis

Medline ID
PubMed ID 8131846
Journal FEBS Lett
Year 1994
Volume 340
Pages 202-6
Authors Markovic-Housley Z, Balbach J, Stolz B, Genovesio-Taverne JC
Title Predicted topology of the N-terminal domain of the hydrophilic subunit of the mannose transporter of Escherichia coli.
Related PDB
Related UniProtKB P69797
Medline ID
PubMed ID 8676384
Journal J Mol Biol
Year 1996
Volume 259
Pages 502-11
Authors Nunn RS, Markovic-Housley Z, Genovesio-Taverne JC, Flukiger K, Rizkallah PJ, Jansonius JN, Schirmer T, Erni B
Title Structure of the IIA domain of the mannose transporter from Escherichia coli at 1.7 angstroms resolution.
Related PDB 1pdo
Related UniProtKB P69797
Medline ID
PubMed ID 9074635
Journal FEBS Lett
Year 1997
Volume 404
Pages 45-50
Authors Gschwind RM, Gemmecker G, Leutner M, Kessler H, Gutknecht R, Lanz R, Flukiger K, Erni B
Title Secondary structure of the IIB domain of the Escherichia coli mannose transporter, a new fold in the class of alpha/beta twisted open-sheet structures.
Related PDB
Related UniProtKB P69797

The same E.C. number ( appears in S00297, D00525, S00283, S00420. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, a phosphoryl group is transferred from phosphoenolpyruvate (PEP) via the PTS enzymes, EI, HPr, IIA, IIB to the tranported sugar.
This enzyme is composed of IIA domain and IIB domain of a mannose transporter (IIAB-mannose).
In the IIA domain, His10 is hydrogen-bonded to a buried Asp67 that is not charge compensated. The carboxylate group of Asp67 acts as a general base and increases the basic strength of the nearby imidazole ring of His10. The nucleophilic attack of the exposed imidazole NE atom onto the phosphorus of phospho-HPr would thus be facilitated.

Created Updated
2002-07-27 2009-02-26