DB code: D00525

RLCP classification 3.105.250000.48 : Transfer
CATH domain -.-.-.- :
3.30.1360.60 : Gyrase A; domain 2 Catalytic domain

CATH domain Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB Protein name Synonyms Includes RefSeq Pfam
P69786 PTS system glucose-specific EIICB component
Glucose permease IIC component PTS system glucose-specific EIIC component
Glucose-specific phosphotransferase enzyme IIB component
PTS system glucose-specific EIIB component
NP_415619.1 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_489369.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF00367 (PTS_EIIB)
PF02378 (PTS_EIIC)
[Graphical View]

KEGG enzyme name
protein-Npi-phosphohistidine---sugar phosphotransferase
glucose permease
PTS permease
phosphotransferase, phosphohistidinoprotein-hexose
enzyme IIl4ac
gene glC proteins
gene bglC RNA formation factors
PEP-dependent phosphotransferase enzyme II
PEP-sugar phosphotransferase enzyme II
phosphoenolpyruvate-sugar phosphotransferase enzyme II
phosphohistidinoprotein-hexose phosphotransferase
phosphohistidinoprotein-hexose phosphoribosyltransferase
phosphoprotein factor-hexose phosophotransferase
protein, specific or class, gene bglC
ribonucleic acid formation factor, gene glC
sucrose phosphotransferase system II
protein-Npi-phosphohistidine:sugar N-pros-phosphotransferase
protein-Npi-phosphohistidine:sugar Npi-phosphotransferase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P69786 PTGCB_ECOLI Protein EIIB N(pi)-phospho-L- histidine/cysteine + sugar = protein EIIB + sugar phosphate. Cell inner membrane, Multi-pass membrane protein.

KEGG Pathways
Map code Pathways E.C.
MAP00010 Glycolysis / Gluconeogenesis
MAP00051 Fructose and mannose metabolism
MAP00052 Galactose metabolism
MAP00053 Ascorbate and aldarate metabolism
MAP00500 Starch and sucrose metabolism
MAP00530 Aminosugars metabolism

Compound table
Substrates Products Intermediates
KEGG-id C04261 C11477 C00615 C00934
Compound Protein N(pi)-phospho-L-histidine Sugar Protein histidine Sugar phosphate
Type aromatic ring (with nitrogen atoms),peptide/protein,phosphate group/phosphate ion polysaccharide aromatic ring (with nitrogen atoms),peptide/protein phosphate group/phosphate ion,polysaccharide
1ibaA Unbound Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
Swiss-prot;P69786, literature [3]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1ibaA CYS 35;ARG 38;ARG 40 CYS 35(phosphorylated)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
p.7411, p.7413-7416

Medline ID
PubMed ID 10736174
Journal Biochemistry
Year 2000
Volume 39
Pages 3745-50
Authors Beutler R, Kaufmann M, Ruggiero F, Erni B
Title The glucose transporter of the Escherichia coli phosphotransferase system: linker insertion mutants and split variants.
Related PDB
Related UniProtKB
Medline ID
PubMed ID 9200688
Journal Biochemistry
Year 1997
Volume 36
Pages 7408-17
Authors Gemmecker G, Eberstadt M, Buhr A, Lanz R, Grdadolnik SG, Kessler H, Erni B
Title Glucose transporter of Escherichia coli: NMR characterization of the phosphocysteine form of the IIB(Glc) domain and its binding interface with the IIA(Glc) subunit.
Related PDB
Related UniProtKB P69786
Medline ID
PubMed ID 8784182
Journal Biochemistry
Year 1996
Volume 35
Pages 11286-92
Authors Eberstadt M, Grdadolnik SG, Gemmecker G, Kessler H, Buhr A, Erni B
Title Solution structure of the IIB domain of the glucose transporter of Escherichia coli.
Related PDB 1iba
Related UniProtKB P69786
Medline ID
PubMed ID 8112346
Journal Eur J Biochem
Year 1994
Volume 219
Pages 945-52
Authors Golic Grdadolnik S, Eberstadt M, Gemmecker G, Kessler H, Buhr A, Erni B
Title The glucose transporter of Escherichia coli. Assignment of the 1H, 13C and 15N resonances and identification of the secondary structure of the soluble IIB domain.
Related PDB
Related UniProtKB P69786
Medline ID
PubMed ID 8505292
Journal J Biol Chem
Year 1993
Volume 268
Pages 11604-9
Authors Meins M, Jeno P, Muller D, Richter WJ, Rosenbusch JP, Erni B
Title Cysteine phosphorylation of the glucose transporter of Escherichia coli.
Related PDB
Related UniProtKB P69786

The same E.C. number ( appears in S00297, D00527, S00283, S00420, S00046. All of them are enzymes in PTS system.
In the phosphotransferase (PTS) system, phosphoryl groups are sequentially transferred from PEP to the transported sugar. The IIB-glucose domain of IICB-glucose subunit is phosphorylated at a cysteine by IIA-glucose domain, which is phosphorylated by HPr protein. Next, the IIA domain will phosphorylate glucose. (see Fig.1 of [2])

Created Updated
2002-07-27 2009-03-04