DB code: D00256
| CATH domain | 1.10.580.10 : Citrate Synthase; domain 1 | Catalytic domain |
|---|---|---|
| 1.10.230.10 : Cytochrome p450-Terp; domain 2 | Catalytic domain | |
| E.C. | 2.3.3.1 | |
| CSA | 1aj8 1al6 | |
| M-CSA | 1aj8 1al6 | |
| MACiE | M0078 | |
| CATH domain | Related DB codes (homologues) |
|---|
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| Q53554 |
Citrate synthase
|
EC
2.3.3.1
|
NP_577932.1
(Protein)
NC_003413.1 (DNA/RNA sequence) |
PF00285
(Citrate_synt)
[Graphical View] |
| P23007 |
Citrate synthase, mitochondrial
|
EC
2.3.3.1
|
PF00285
(Citrate_synt)
[Graphical View] |
|
| P00889 |
Citrate synthase, mitochondrial
|
EC
2.3.3.1
|
NP_999441.1
(Protein)
NM_214276.1 (DNA/RNA sequence) |
PF00285
(Citrate_synt)
[Graphical View] |
| KEGG enzyme name |
|---|
|
citrate (Si)-synthase
(R)-citric synthase citrate condensing enzyme citrate oxaloacetate-lyase [(pro-3S)-CH2COO-->acetyl-CoA] citrate oxaloacetate-lyase, CoA-acetylating citrate synthase citrate synthetase citric synthase citric-condensing enzyme citrogenase condensing enzyme oxaloacetate transacetase oxalacetic transacetase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| Q53554 | CISY_PYRFU | Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA. | Homodimer. | ||
| P23007 | CISY_CHICK | Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA. | Homodimer. | Mitochondrion matrix. | |
| P00889 | CISY_PIG | Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA. | Homodimer. | Mitochondrion matrix. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00020 | Citrate cycle (TCA cycle) | |
| MAP00630 | Glyoxylate and dicarboxylate metabolism |
| Compound table | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | |||||||||||
| KEGG-id | C00024 | C00001 | C00036 | C00010 | C00158 | C00566 | |||||||
| E.C. | |||||||||||||
| Compound | Acetyl-CoA | H2O | Oxaloacetate | CoA | Citrate | Enolic acetyl-CoA | Citryl-CoA | Enzyme-anhydride intermediate | |||||
| Type | amine group,carbohydrate,nucleotide ,peptide/protein,sulfide group | H2O | carbohydrate,carboxyl group | amine group,carbohydrate,nucleotide ,peptide/protein,sulfhydryl group | carbohydrate,carboxyl group | ||||||||
| ChEBI |
15351 15351 |
15377 15377 |
30744 30744 |
15346 15346 |
30769 30769 |
||||||||
| PubChem |
444493 6302 444493 6302 |
22247451 962 22247451 962 |
970 970 |
6816 87642 6816 87642 |
19782904 311 88113319 19782904 311 88113319 |
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| 1aj8A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1aj8B01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1al6A01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1amzA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1cscA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1cshA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1csiA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1csrA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1cssA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 2cscA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 3cscA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 4cscA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 5cscA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 5cscB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 5ctsA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 6cscA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 6cscB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 6ctsA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1ctsA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 2ctsA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 4ctsA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 4ctsB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 1aj8A02 |
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Unbound | Unbound | Bound:COA | Bound:CIT | Unbound | |||
| 1aj8B02 |
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Unbound | Unbound | Bound:COA | Bound:CIT | Unbound | |||
| 1al6A02 |
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Analogue:HAX | Bound:OAA | Unbound | Unbound | Unbound | |||
| 1amzA02 |
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Analogue:NMX | Analogue:MLT | Unbound | Unbound | Unbound | |||
| 1cscA02 |
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Analogue:CMC | Analogue:MAL | Unbound | Unbound | Unbound | |||
| 1cshA02 |
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Analogue:AMX | Bound:OAA | Unbound | Unbound | Unbound | |||
| 1csiA02 |
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Analogue:CMX | Bound:OAA | Unbound | Unbound | Unbound | |||
| 1csrA02 |
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Analogue:FAM | Bound:OAA | Unbound | Unbound | Unbound | |||
| 1cssA02 |
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Analogue:FCX | Bound:OAA | Unbound | Unbound | Unbound | |||
| 2cscA02 |
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Analogue:CMC | Analogue:MAL | Unbound | Unbound | Unbound | |||
| 3cscA02 |
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Bound:ACO | Analogue:MAL | Unbound | Unbound | Unbound | |||
| 4cscA02 |
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Bound:ACO | Analogue:MAL | Unbound | Unbound | Unbound | |||
| 5cscA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 5cscB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 5ctsA02 |
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Analogue:CMC | Bound:OAA | Unbound | Unbound | Unbound | |||
| 6cscA02 |
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Analogue:COF | Unbound | Unbound | Bound:CIT | Unbound | |||
| 6cscB02 |
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Analogue:COF | Unbound | Unbound | Bound:CIT | Unbound | |||
| 6ctsA02 |
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Unbound | Unbound | Unbound | Unbound | Intermediate-analogue:CIC | |||
| 1ctsA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | |||
| 2ctsA02 |
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Unbound | Unbound | Bound:COA | Bound:CIT | Unbound | |||
| 4ctsA02 |
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Unbound | Bound:OAA | Unbound | Unbound | Unbound | |||
| 4ctsB02 |
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Unbound | Bound:OAA | Unbound | Unbound | Unbound | |||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P23007, P00889, Q53554 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1aj8A01 |
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HIS 223 | ||||
| 1aj8B01 |
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HIS 223 | ||||
| 1al6A01 |
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HIS 274 | ||||
| 1amzA01 |
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HIS 274 | ||||
| 1cscA01 |
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HIS 274 | ||||
| 1cshA01 |
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HIS 274 | ||||
| 1csiA01 |
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HIS 274 | ||||
| 1csrA01 |
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HIS 274 | ||||
| 1cssA01 |
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HIS 274 | ||||
| 2cscA01 |
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HIS 274 | ||||
| 3cscA01 |
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HIS 274 | ||||
| 4cscA01 |
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HIS 274 | ||||
| 5cscA01 |
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HIS 274 | ||||
| 5cscB01 |
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HIS 274 | ||||
| 5ctsA01 |
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HIS 274 | ||||
| 6cscA01 |
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HIS 274 | ||||
| 6cscB01 |
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HIS 274 | ||||
| 6ctsA01 |
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HIS 274 | ||||
| 1ctsA01 |
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HIS 274 | ||||
| 2ctsA01 |
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HIS 274 | ||||
| 4ctsA01 |
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HIS 274 | ||||
| 4ctsB01 |
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HIS 274 | ||||
| 1aj8A02 |
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HIS 262;ASP 312 | ||||
| 1aj8B02 |
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HIS 262;ASP 312 | ||||
| 1al6A02 |
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HIS 320;ASP 375 | ||||
| 1amzA02 |
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HIS 320;ASP 375 | ||||
| 1cscA02 |
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HIS 320;ASP 375 | ||||
| 1cshA02 |
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HIS 320;ASP 375 | ||||
| 1csiA02 |
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HIS 320;ASP 375 | ||||
| 1csrA02 |
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HIS 320;ASP 375 | ||||
| 1cssA02 |
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HIS 320;ASP 375 | ||||
| 2cscA02 |
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HIS 320;ASP 375 | ||||
| 3cscA02 |
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HIS 320;ASP 375 | ||||
| 4cscA02 |
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HIS 320;ASP 375 | ||||
| 5cscA02 |
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HIS 320;ASP 375 | ||||
| 5cscB02 |
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HIS 320;ASP 375 | ||||
| 5ctsA02 |
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HIS 320;ASP 375 | ||||
| 6cscA02 |
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HIS 320;ASP 375 | ||||
| 6cscB02 |
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HIS 320;ASP 375 | ||||
| 6ctsA02 |
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HIS 320;ASP 375 | ||||
| 1ctsA02 |
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HIS 320;ASP 375 | LYS 368(N6,N6,N6-trimethyllysine) | |||
| 2ctsA02 |
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HIS 320;ASP 375 | LYS 368(N6,N6,N6-trimethyllysine) | |||
| 4ctsA02 |
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HIS 320;ASP 375 | LYS 368(N6,N6,N6-trimethyllysine) | |||
| 4ctsB02 |
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HIS 320;ASP 375 | LYS 368(N6,N6,N6-trimethyllysine) | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[6]
|
p.149-150 | |
|
[7]
|
Scheme 2, p.671 | 2 |
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[14]
|
Fig.3, p.7561-7562 | 4 |
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[15]
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Fig.4, p.2216 | 2 |
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[16]
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Scheme 3, p.711-712 | 3 |
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[17]
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Scheme 2, p.524-526 | 3 |
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[18]
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p.6030 | |
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[21]
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p.7912-7914 | |
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[22]
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p.7906-7907 | |
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[23]
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Scheme 2, Scheme 3, Scheme 4, p.226 | 5 |
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[27]
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p.769-770 | |
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[29]
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Fig.2, p.7757-7759 | 3 |
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[35]
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Fig.1, p.10667-10671 | 3 |
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[39]
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p.9987 | |
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[45]
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||
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1278369 |
| Journal | FEBS Lett |
| Year | 1976 |
| Volume | 62 |
| Pages | 281-3 |
| Authors | Wiegand G |
| Title | Crystallization and preliminary x-ray data of well-ordered crystals from pig heart citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 666830 |
| Journal | Biochem Biophys Res Commun |
| Year | 1978 |
| Volume | 82 |
| Pages | 150-6 |
| Authors | Wang AH, Sherman ML, Rich A |
| Title | A crystallographic investigation of citrate synthase from pig and chicken heart muscle. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 224920 |
| Journal | Biochemistry |
| Year | 1979 |
| Volume | 18 |
| Pages | 3822-7 |
| Authors | Weidman SW, Drysdale GR |
| Title | Interaction of a paramagnetic analogue of oxaloacetate with citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 436830 |
| Journal | Eur J Biochem |
| Year | 1979 |
| Volume | 93 |
| Pages | 41-50 |
| Authors | Wiegand G, Kukla D, Scholze H, Jones TA, Huber R |
| Title | Crystal structure analysis of the tetragonal crystal form are preliminary molecular model of pig-heart citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 218954 |
| Journal | J Biol Chem |
| Year | 1979 |
| Volume | 254 |
| Pages | 2800-6 |
| Authors | Caggiano AV, Powell GL |
| Title |
Regulation of enzymes by fatty acyl coenzyme A. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.7 AND 1.7 ANGSTROMS). |
| Medline ID | 83010291 |
| PubMed ID | 7120407 |
| Journal | J Mol Biol |
| Year | 1982 |
| Volume | 158 |
| Pages | 111-52 |
| Authors | Remington S, Wiegand G, Huber R |
| Title | Crystallographic refinement and atomic models of two different forms of citrate synthase at 2.7 and 1.7 A resolution. |
| Related PDB | 1cts 2cts |
| Related UniProtKB | P00889 |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6861748 |
| Journal | Eur J Biochem |
| Year | 1983 |
| Volume | 133 |
| Pages | 665-72 |
| Authors | Lohlein-Werhahn G, Bayer E, Bauer B, Eggerer H |
| Title |
Hysteretic behaviour of citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6337135 |
| Journal | J Biol Chem |
| Year | 1983 |
| Volume | 258 |
| Pages | 1297-8 |
| Authors | Rubin BH, Stallings WC, Glusker JP, Bayer ME, Janin J, Srere PA |
| Title | Crystallographic studies of Escherichia coli citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 6716477 |
| Journal | J Mol Biol |
| Year | 1984 |
| Volume | 174 |
| Pages | 205-19 |
| Authors | Wiegand G, Remington S, Deisenhofer J, Huber R |
| Title | Crystal structure analysis and molecular model of a complex of citrate synthase with oxaloacetate and S-acetonyl-coenzyme A. |
| Related PDB | 4cts |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3978085 |
| Journal | Biochemistry |
| Year | 1985 |
| Volume | 24 |
| Pages | 452-7 |
| Authors | Kurz LC, Ackerman JJ, Drysdale GR |
| Title | Evidence from 13C NMR for polarization of the carbonyl of oxaloacetate in the active site of citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3607038 |
| Journal | Biochemistry |
| Year | 1987 |
| Volume | 26 |
| Pages | 2623-7 |
| Authors | Kurz LC, Drysdale GR |
| Title | Evidence from Fourier transform infrared spectroscopy for polarization of the carbonyl of oxaloacetate in the active site of citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2731546 |
| Journal | Eur J Biochem |
| Year | 1989 |
| Volume | 182 |
| Pages | 119-24 |
| Authors | Pettersson G, Lill U, Eggerer H |
| Title | Mechanism of interaction of citrate synthase with citryl-CoA. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2276456 |
| Journal | Biochem Soc Trans |
| Year | 1990 |
| Volume | 18 |
| Pages | 596-7 |
| Authors | Iles RA, Davies SE, Chalmers RA, Wharton CW, White A, Sreedharan S, Phillips I, Brocklehurst K |
| Title | Structural and mechanistic studies on citrate synthase by nuclear magnetic resonance and Fourier transform infra-red spectroscopies. |
| Related PDB | |
| Related UniProtKB | |
| [14] | |
| Resource | |
| Comments | MUTAGENESIS. |
| Medline ID | 91104711 |
| PubMed ID | 1702991 |
| Journal | Biochemistry |
| Year | 1990 |
| Volume | 29 |
| Pages | 7557-63 |
| Authors | Alter GM, Casazza JP, Zhi W, Nemeth P, Srere PA, Evans CT |
| Title | Mutation of essential catalytic residues in pig citrate synthase. |
| Related PDB | |
| Related UniProtKB | P00889 |
| [15] | |
| Resource | |
| Comments | TISSUE=Heart muscle; |
| Medline ID | 90248434 |
| PubMed ID | 2337600 |
| Journal | Biochemistry |
| Year | 1990 |
| Volume | 29 |
| Pages | 2213-9 |
| Authors | Karpusas M, Branchaud B, Remington SJ |
| Title | Proposed mechanism for the condensation reaction of citrate synthase: 1.9-A structure of the ternary complex with oxaloacetate and carboxymethyl coenzyme A. |
| Related PDB | 5cts 6cts |
| Related UniProtKB | P23007 |
| [16] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2206458 |
| Journal | Biol Chem Hoppe Seyler |
| Year | 1990 |
| Volume | 371 |
| Pages | 707-13 |
| Authors | Wilde J, Lill U, Eggerer H |
| Title | On the action of carboxy groups in the citrate synthase reaction. |
| Related PDB | |
| Related UniProtKB | |
| [17] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1684105 |
| Journal | Biochem J |
| Year | 1991 |
| Volume | 280 |
| Pages | 521-6 |
| Authors | Man WJ, Li Y, O'Connor CD, Wilton DC |
| Title | Conversion of citrate synthase into citryl-CoA lyase as a result of mutation of the active-site aspartic acid residue to glutamic acid. |
| Related PDB | |
| Related UniProtKB | |
| [18] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 2043640 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 6024-31 |
| Authors | Karpusas M, Holland D, Remington SJ |
| Title | 1.9-A structures of ternary complexes of citrate synthase with D- and L-malate: mechanistic implications. |
| Related PDB | 1csc 2csc 3csc 4csc |
| Related UniProtKB | |
| [19] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF OPEN CONFORMATION. |
| Medline ID | 91255228 |
| PubMed ID | 2043641 |
| Journal | Biochemistry |
| Year | 1991 |
| Volume | 30 |
| Pages | 6031-6 |
| Authors | Liao DI, Karpusas M, Remington SJ |
| Title | Crystal structure of an open conformation of citrate synthase from chicken heart at 2.8-A resolution. |
| Related PDB | 5csc |
| Related UniProtKB | P23007 |
| [20] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1939121 |
| Journal | J Biol Chem |
| Year | 1991 |
| Volume | 266 |
| Pages | 20709-13 |
| Authors | Donald LJ, Crane BR, Anderson DH, Duckworth HW |
| Title |
The role of cysteine 206 in allosteric inhibition of Escherichia coli citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [21] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1324723 |
| Journal | Biochemistry |
| Year | 1992 |
| Volume | 31 |
| Pages | 7908-14 |
| Authors | Kurz LC, Drysdale GR, Riley MC, Evans CT, Srere PA |
| Title | Catalytic strategy of citrate synthase: effects of amino acid changes in the acetyl-CoA binding site on transition-state analog inhibitor complexes. |
| Related PDB | |
| Related UniProtKB | |
| [22] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1324722 |
| Journal | Biochemistry |
| Year | 1992 |
| Volume | 31 |
| Pages | 7899-907 |
| Authors | Kurz LC, Shah S, Crane BR, Donald LJ, Duckworth HW, Drysdale GR |
| Title |
Proton uptake accompanies formation of the ternary complex of citrate synthase, |
| Related PDB | |
| Related UniProtKB | |
| [23] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1499334 |
| Journal | Curr Top Cell Regul |
| Year | 1992 |
| Volume | 33 |
| Pages | 209-29 |
| Authors | Remington SJ |
| Title | Structure and mechanism of citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [24] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8098211 |
| Journal | Biochem J |
| Year | 1993 |
| Volume | 291 |
| Pages | 927-32 |
| Authors | Evans CT, Sumegi B, Srere PA, Sherry AD, Malloy CR |
| Title | 13C]propionate oxidation in wild-type and citrate synthase mutant Escherichia coli: evidence for multiple pathways of propionate utilization. |
| Related PDB | |
| Related UniProtKB | |
| [25] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8331668 |
| Journal | J Mol Biol |
| Year | 1993 |
| Volume | 232 |
| Pages | 308-9 |
| Authors | Russell RJ, Byrom D, Danson MJ, Hough DW, Taylor GL |
| Title | Crystallization and preliminary crystallographic study of citrate synthase from the thermophilic Archaeon Thermoplasma acidophilum. |
| Related PDB | |
| Related UniProtKB | |
| [26] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8356034 |
| Journal | Proteins |
| Year | 1993 |
| Volume | 16 |
| Pages | 393-407 |
| Authors | Ech-Cherif el-Kettani MA, Zakrzewska K, Durup J, Lavery R |
| Title | An analysis of the conformational paths of citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [27] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8010958 |
| Journal | Biochem J |
| Year | 1994 |
| Volume | 300 |
| Pages | 765-70 |
| Authors | Man WJ, Li Y, O'Connor CD, Wilton DC |
| Title |
The effect of replacing the conserved active-site residues His-264, |
| Related PDB | |
| Related UniProtKB | |
| [28] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7918384 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 11684-91 |
| Authors | Lindbladh C, Brodeur RD, Small WC, Lilius G, Bulow L, Mosbach K, Srere PA |
| Title | Metabolic studies on Saccharomyces cerevisiae containing fused citrate synthase/malate dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [29] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8011640 |
| Journal | Biochemistry |
| Year | 1994 |
| Volume | 33 |
| Pages | 7753-9 |
| Authors | Usher KC, Remington SJ, Martin DP, Drueckhammer DG |
| Title | A very short hydrogen bond provides only moderate stabilization of an enzyme-inhibitor complex of citrate synthase. |
| Related PDB | 1csh 1csi |
| Related UniProtKB | |
| [30] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8276829 |
| Journal | J Biol Chem |
| Year | 1994 |
| Volume | 269 |
| Pages | 412-7 |
| Authors | Pereira DS, Donald LJ, Hosfield DJ, Duckworth HW |
| Title |
Active site mutants of Escherichia coli citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [31] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7704526 |
| Journal | Structure |
| Year | 1994 |
| Volume | 2 |
| Pages | 1157-67 |
| Authors | Russell RJ, Hough DW, Danson MJ, Taylor GL |
| Title |
The crystal structure of citrate synthase from the thermophilic archaeon, |
| Related PDB | |
| Related UniProtKB | |
| [32] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7577912 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 13278-88 |
| Authors | Kurz LC, Shah S, Frieden C, Nakra T, Stein RE, Drysdale GR, Evans CT, Srere PA |
| Title | Catalytic strategy of citrate synthase: subunit interactions revealed as a consequence of a single amino acid change in the oxaloacetate binding site. |
| Related PDB | |
| Related UniProtKB | |
| [33] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 7492547 |
| Journal | Biochemistry |
| Year | 1995 |
| Volume | 34 |
| Pages | 15459-66 |
| Authors | Schwartz B, Drueckhammer DG, Usher KC, Remington SJ |
| Title | alpha-Fluoro acid and alpha-fluoro amide analogs of acetyl-CoA as inhibitors of citrate synthase: effect of pKa matching on binding affinity and hydrogen bond length. |
| Related PDB | 1csr 1css |
| Related UniProtKB | |
| [34] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9052973 |
| Journal | J Mol Recognit |
| Year | 1995 |
| Volume | 8 |
| Pages | 327-33 |
| Authors | Evans CT |
| Title | Metabolic engineering of a non-allosteric citrate synthase in an Escherichia coli citrate synthase mutant. |
| Related PDB | |
| Related UniProtKB | |
| [35] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8718855 |
| Journal | Biochemistry |
| Year | 1996 |
| Volume | 35 |
| Pages | 10661-72 |
| Authors | Evans CT, Kurz LC, Remington SJ, Srere PA |
| Title | Active site mutants of pig citrate synthase: effects of mutations on the enzyme catalytic and structural properties. |
| Related PDB | |
| Related UniProtKB | |
| [36] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8979399 |
| Journal | Plant Cell Physiol |
| Year | 1996 |
| Volume | 37 |
| Pages | 1022-9 |
| Authors | La Cognata U, Landschutze V, Willmitzer L, Muller-Rober B |
| Title | Structure and expression of mitochondrial citrate synthases from higher plants. |
| Related PDB | |
| Related UniProtKB | |
| [37] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9388613 |
| Journal | Biochem Soc Trans |
| Year | 1997 |
| Volume | 25 |
| Pages | 380S |
| Authors | Severcan F, Stenland C, Millhauser G |
| Title | ESR studies of pig citrate synthase. |
| Related PDB | |
| Related UniProtKB | |
| [38] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 9092828 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 3981-90 |
| Authors | Kurz LC, Roble JH, Nakra T, Drysdale GR, Buzan JM, Schwartz B, Drueckhammer DG |
| Title |
Ability of single-site mutants of citrate synthase to catalyze proton transfer from the methyl group of dethiaacetyl-coenzyme A, |
| Related PDB | 1al6 1amz |
| Related UniProtKB | |
| [39] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). |
| Medline ID | 97400454 |
| PubMed ID | 9254593 |
| Journal | Biochemistry |
| Year | 1997 |
| Volume | 36 |
| Pages | 9983-94 |
| Authors | Russell RJ, Ferguson JM, Hough DW, Danson MJ, Taylor GL |
| Title | The crystal structure of citrate synthase from the hyperthermophilic archaeon pyrococcus furiosus at 1.9 A resolution,. |
| Related PDB | 1aj8 |
| Related UniProtKB | Q53554 |
| [40] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9757123 |
| Journal | Acta Crystallogr D Biol Crystallogr |
| Year | 1998 |
| Volume | 54 |
| Pages | 1012-3 |
| Authors | Gerike U, Russell RJ, Danson MJ, Russell NJ, Hough DW, Taylor GL |
| Title | Preliminary crystallographic studies of citrate synthase from an Antarctic psychrotolerant bacterium. |
| Related PDB | |
| Related UniProtKB | |
| [41] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9657685 |
| Journal | Biochemistry |
| Year | 1998 |
| Volume | 37 |
| Pages | 9724-37 |
| Authors | Kurz LC, Nakra T, Stein R, Plungkhen W, Riley M, Hsu F, Drysdale GR |
| Title | Effects of changes in three catalytic residues on the relative stabilities of some of the intermediates and transition states in the citrate synthase reaction. |
| Related PDB | |
| Related UniProtKB | |
| [42] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10387046 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 8022-31 |
| Authors | Gu Z, Drueckhammer DG, Kurz L, Liu K, Martin DP, McDermott A |
| Title | Solid state NMR studies of hydrogen bonding in a citrate synthase inhibitor complex. |
| Related PDB | |
| Related UniProtKB | |
| [43] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9893982 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 881-9 |
| Authors | Shatalin K, Lebreton S, Rault-Leonardon M, Velot C, Srere PA |
| Title | Electrostatic channeling of oxaloacetate in a fusion protein of porcine citrate synthase and porcine mitochondrial malate dehydrogenase. |
| Related PDB | |
| Related UniProtKB | |
| [44] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9933583 |
| Journal | J Biol Chem |
| Year | 1999 |
| Volume | 274 |
| Pages | 3941-5 |
| Authors | Haggie PM, Brindle KM |
| Title | Mitochondrial citrate synthase is immobilized in vivo. |
| Related PDB | |
| Related UniProtKB | |
| [45] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10694395 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 2283-96 |
| Authors | Kurz LC, Drysdale G, Riley M, Tomar MA, Chen J, Russell RJ, Danson MJ |
| Title | Kinetics and mechanism of the citrate synthase from the thermophilic archaeon Thermoplasma acidophilum. |
| Related PDB | |
| Related UniProtKB | |
| [46] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11501994 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 310 |
| Pages | 1039-53 |
| Authors | Roccatano D, Mark AE, Hayward S |
| Title | Investigation of the mechanism of domain closure in citrate synthase by molecular dynamics simulation. |
| Related PDB | |
| Related UniProtKB | |
| [47] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11707611 |
| Journal | Protein Eng |
| Year | 2001 |
| Volume | 14 |
| Pages | 655-61 |
| Authors | Gerike U, Danson MJ, Hough DW |
| Title | Cold-active citrate synthase: mutagenesis of active-site residues. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme catalyzes the following reactions sequentially:
(A) Isomerization (enolization) (B) Condensation (Addition) (C) Hydrolysis of thioester group |
| Created | Updated |
|---|---|
| 2004-06-01 | 2009-02-26 |