DB code: D00254

CATH domain 1.10.12.10 : Lyase 2-enoyl-coa Hydratase; Chain A, domain 2
3.90.226.10 : 2-enoyl-CoA Hydratase; Chain A, domain 1 Catalytic domain
E.C. 4.1.1.41
CSA 1ef8
M-CSA 1ef8
MACiE M0070

CATH domain Related DB codes (homologues)
3.90.226.10 : 2-enoyl-CoA Hydratase; Chain A, domain 1 M00145 M00122 S00849

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P52045 Methylmalonyl-CoA decarboxylase
MMCD
EC 4.1.1.41
Transcarboxylase
NP_417394.4 (Protein)
NC_000913.2 (DNA/RNA sequence)
YP_491119.1 (Protein)
NC_007779.1 (DNA/RNA sequence)
PF00378 (ECH)
[Graphical View]

KEGG enzyme name
methylmalonyl-CoA decarboxylase
propionyl-CoA carboxylase
propionyl coenzyme A carboxylase
methylmalonyl-coenzyme A decarboxylase
(S)-2-methyl-3-oxopropanoyl-CoA carboxy-lyase [incorrect]
(S)-methylmalonyl-CoA carboxy-lyase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P52045 MMCD_ECOLI (S)-methylmalonyl-CoA = propanoyl-CoA + CO(2). Dimer of homotrimers.

KEGG Pathways
Map code Pathways E.C.
MAP00640 Propanoate metabolism

Compound table
Cofactors Substrates Products Intermediates
KEGG-id C00120 C00683 C00100 C00011
E.C.
Compound Biotin (S)-2-Methyl-3-oxopropanoyl-CoA Propanoyl-CoA CO2
Type amide group,amine group,fatty acid,sulfide group amine group,carbohydrate,carboxyl group,nucleotide ,peptide/protein,sulfide group amine group,carbohydrate,nucleotide ,peptide/protein,sulfide group others
ChEBI 15956
 15956
 		15466
 15466
 		15539
 15539
 		16526
 16526
PubChem 171548
 171548
 		11966111
 439291
 11966111
 439291
 		439164
 92753
 439164
 92753
 		280
 280
1ef8A01 Unbound Unbound Unbound Unbound
1ef8B01 Unbound Unbound Unbound Unbound
1ef8C01 Unbound Unbound Unbound Unbound
1ef9A01 Unbound Unbound Unbound Unbound
1ef8A02 Unbound Unbound Unbound Unbound
1ef8B02 Unbound Unbound Unbound Unbound
1ef8C02 Unbound Unbound Unbound Unbound
1ef9A02 Unbound Analogue:2CP Unbound Unbound

Reference for Active-site residues
resource references E.C.
PDB;1ef8 & literature [12]

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1ef8A01 HIS 220(Nickel binding)
1ef8B01 HIS 220(Nickel binding)
1ef8C01 HIS 220(Nickel binding)
1ef9A01
1ef8A02 TYR 140 HIS 66;GLY 110
1ef8B02 TYR 140 HIS 66;GLY 110
1ef8C02 TYR 140 HIS 66;GLY 110
1ef9A02 TYR 140 HIS 66;GLY 110

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[3]
Fig.1, Fig.6 2
[12]
Fig.6, p.4638-4639 2

References
[1]
Resource
Comments
Medline ID
PubMed ID 6852015
Journal Eur J Biochem
Year 1983
Volume 132
Pages 579-87
Authors Hilpert W, Dimroth P
Title Purification and characterization of a new sodium-transport decarboxylase. Methylmalonyl-CoA decarboxylase from Veillonella alcalescens.
Related PDB
Related UniProtKB
[2]
Resource
Comments
Medline ID
PubMed ID 6705792
Journal Eur J Biochem
Year 1984
Volume 140
Pages 147-51
Authors Haase FC, Beegen H, Allen SH
Title Propionyl-coenzyme A carboxylase of Mycobacterium smegmatis. An electron microscopic study.
Related PDB
Related UniProtKB
[3]
Resource
Comments
Medline ID
PubMed ID 3860170
Journal Ann N Y Acad Sci
Year 1985
Volume 447
Pages 140-51
Authors Fry DC, Fox T, Lane MD, Mildvan AS
Title NMR studies of the exchange of the amide protons of d-biotin and its derivatives.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 3464942
Journal Proc Natl Acad Sci U S A
Year 1986
Volume 83
Pages 8049-53
Authors Kraus JP, Firgaira F, Novotny J, Kalousek F, Williams KR, Williamson C, Ohura T, Rosenberg LE
Title Coding sequence of the precursor of the beta subunit of rat propionyl-CoA carboxylase.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID 3609308
Journal FEBS Lett
Year 1987
Volume 220
Pages 121-5
Authors Hoffmann A, Dimroth P
Title Stereochemistry of the methylmalonyl-CoA decarboxylation reaction.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID 1769208
Journal Comp Biochem Physiol B
Year 1991
Volume 99
Pages 613-7
Authors Garrastazu C, Iniesta MP, Aranguez MI, Ruiz Amil M
Title Comparative analysis of propionyl-CoA carboxylase from liver and mammary gland of mid-lactation cow.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID 8227015
Journal J Biol Chem
Year 1993
Volume 268
Pages 24564-71
Authors Huder JB, Dimroth P
Title Sequence of the sodium ion pump methylmalonyl-CoA decarboxylase from Veillonella parvula.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID 7601825
Journal J Bacteriol
Year 1995
Volume 177
Pages 3623-30
Authors Huder JB, Dimroth P
Title Expression of the sodium ion pump methylmalonyl-coenzyme A-decarboxylase from Veillonella parvula and of mutated enzyme specimens in Escherichia coli.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 8865276
Journal Pediatr Res
Year 1996
Volume 40
Pages 404-9
Authors Bergman AJ, Van der Knaap MS, Smeitink JA, Duran M, Dorland L, Valk J, Poll-The BT
Title Magnetic resonance imaging and spectroscopy of the brain in propionic acidemia: clinical and biochemical considerations.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 9683657
Journal Arch Microbiol
Year 1998
Volume 170
Pages 179-84
Authors Kimura Y, Kojyo T, Kimura I, Sato M
Title Propionyl-CoA carboxylase of Myxococcus xanthus: catalytic properties and function in developing cells.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 9887338
Journal Hum Mol Genet
Year 1999
Volume 8
Pages 107-13
Authors Campeau E, Dupuis L, Leclerc D, Gravel RA
Title Detection of a normally rare transcript in propionic acidemia patients with mRNA destabilizing mutations in the PCCA gene.
Related PDB
Related UniProtKB
[12]
Resource
Comments X-ray crystallography
Medline ID
PubMed ID 10769118
Journal Biochemistry
Year 2000
Volume 39
Pages 4630-9
Authors Benning MM, Haller T, Gerlt JA, Holden HM
Title New reactions in the crotonase superfamily: structure of methylmalonyl CoA decarboxylase from Escherichia coli.
Related PDB 1ef8 1ef9
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 10769117
Journal Biochemistry
Year 2000
Volume 39
Pages 4622-9
Authors Haller T, Buckel T, Retey J, Gerlt JA
Title Discovering new enzymes and metabolic pathways: conversion of succinate to propionate by Escherichia coli.
Related PDB
Related UniProtKB
[14]
Resource
Comments
Medline ID
PubMed ID 11136555
Journal Mol Genet Metab
Year 2000
Volume 71
Pages 623-32
Authors Chloupkova M, Ravn K, Schwartz M, Kraus JP
Title Changes in the carboxyl terminus of the beta subunit of human propionyl-CoA carboxylase affect the oligomer assembly and catalysis: expression and characterization of seven patient-derived mutant forms of PCC in Escherichia coli.
Related PDB
Related UniProtKB
[15]
Resource
Comments
Medline ID
PubMed ID 11414279
Journal Mol Neurobiol
Year 2000
Volume 22
Pages 21-40
Authors Hassel B
Title Carboxylation and anaplerosis in neurons and glia.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-05-21 2009-02-26