DB code: D00144

RLCP classification 1.19.30000.10 : Hydrolysis
CATH domain 3.40.50.2300 : Rossmann fold
3.40.50.180 : Rossmann fold Catalytic domain
E.C. 3.1.1.61
CSA 1chd
M-CSA 1chd
MACiE

CATH domain Related DB codes (homologues)
3.40.50.2300 : Rossmann fold M00143

Uniprot Enzyme Name
UniprotKB Protein name Synonyms RefSeq Pfam
P04042 Chemotaxis response regulator protein-glutamate methylesterase
EC 3.1.1.61
NP_460874.1 (Protein)
NC_003197.1 (DNA/RNA sequence)
PF01339 (CheB_methylest)
PF00072 (Response_reg)
[Graphical View]

KEGG enzyme name
protein-glutamate methylesterase
chemotaxis-specific methylesterase
methyl-accepting chemotaxis protein methyl-esterase
CheB methylesterase
methylesterase CheB
protein methyl-esterase
protein carboxyl methylesterase
PME
protein methylesterase
protein-L-glutamate-5-O-methyl-ester acylhydrolase

UniprotKB: Accession Number Entry name Activity Subunit Subcellular location Cofactor
P04042 CHEB_SALTY Protein L-glutamate O(5)-methyl ester + H(2)O = protein L-glutamate + methanol. Cytoplasm.

KEGG Pathways
Map code Pathways E.C.

Compound table
Substrates Products Intermediates
KEGG-id C04142 C00001 C00614 C00132
E.C.
Compound Protein glutamate methyl ester H2O Protein glutamate Methanol
Type peptide/protein H2O carboxyl group,peptide/protein carbohydrate
ChEBI 15377
 15377
 		17790
 17790
PubChem 22247451
 962
 22247451
 962
 		887
 887
1a2oA01 Unbound Unbound Unbound
1a2oB01 Unbound Unbound Unbound
1a2oA02 Unbound Unbound Unbound
1a2oB02 Unbound Unbound Unbound
1chdA Unbound Unbound Unbound

Reference for Active-site residues
resource references E.C.
PDB;1a2o & Swiss-prot;P04042

Active-site residues
PDB Catalytic residues Cofactor-binding residues Modified residues Main-chain involved in catalysis Comment
1a2oA01 ASP 56(phosphorylation)
1a2oB01 ASP 56(phosphorylation)
1a2oA02 SER 164;HIS 190;ASP 286 THR 165;MET 283(oxyanion hole)
1a2oB02 SER 164;HIS 190;ASP 286 THR 165;MET 283(oxyanion hole)
1chdA SER 164;HIS 190;ASP 286 THR 165;MET 283(oxyanion hole)

References for Catalytic Mechanism
References Sections No. of steps in catalysis
[2]
Fig.9, p.279-282

References
[1]
Resource
Comments PHOSPHORYLATION.
Medline ID 88165060
PubMed ID 3280143
Journal Cell
Year 1988
Volume 53
Pages 79-87
Authors Hess JF, Oosawa K, Kaplan N, Simon MI
Title Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis.
Related PDB
Related UniProtKB P04042
[2]
Resource
Comments X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 152-349.
Medline ID 95333182
PubMed ID 7608974
Journal J Mol Biol
Year 1995
Volume 250
Pages 276-90
Authors West AH, Martinez-Hackert E, Stock AM
Title Crystal structure of the catalytic domain of the chemotaxis receptor methylesterase, CheB.
Related PDB 1chd
Related UniProtKB P04042
[3]
Resource
Comments
Medline ID
PubMed ID 7567955
Journal Proteins
Year 1995
Volume 21
Pages 345-50
Authors West AH, Djordjevic S, Martinez-Hackert E, Stock AM
Title Purification, crystallization, and preliminary X-ray diffraction analyses of the bacterial chemotaxis receptor modifying enzymes.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID 9139915
Journal J Bacteriol
Year 1997
Volume 179
Pages 2963-8
Authors Brooun A, Zhang W, Alam M
Title Primary structure and functional analysis of the soluble transducer protein HtrXI in the archaeon Halobacterium salinarium.
Related PDB
Related UniProtKB
[5]
Resource
Comments CHARACTERIZATION.
Medline ID 98434376
PubMed ID 9760239
Journal Biochemistry
Year 1998
Volume 37
Pages 14038-47
Authors Anand GS, Goudreau PN, Stock AM
Title Activation of methylesterase CheB: evidence of a dual role for the regulatory domain.
Related PDB
Related UniProtKB P04042
[6]
Resource
Comments
Medline ID
PubMed ID 10049806
Journal J Struct Biol
Year 1998
Volume 124
Pages 189-200
Authors Djordjevic S, Stock AM
Title Structural analysis of bacterial chemotaxis proteins: components of a dynamic signaling system.
Related PDB
Related UniProtKB
[7]
Resource
Comments X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
Medline ID 98132598
PubMed ID 9465023
Journal Proc Natl Acad Sci U S A
Year 1998
Volume 95
Pages 1381-6
Authors Djordjevic S, Goudreau PN, Xu Q, Stock AM, West AH
Title Structural basis for methylesterase CheB regulation by a phosphorylation-activated domain.
Related PDB 1a2o
Related UniProtKB P04042
[8]
Resource
Comments
Medline ID
PubMed ID 10231491
Journal Mol Microbiol
Year 1999
Volume 32
Pages 357-65
Authors Nishiyama SI, Umemura T, Nara T, Homma M, Kawagishi I
Title Conversion of a bacterial warm sensor to a cold sensor by methylation of a single residue in the presence of an attractant.
Related PDB
Related UniProtKB
[9]
Resource
Comments
Medline ID
PubMed ID 10760170
Journal Mol Microbiol
Year 2000
Volume 36
Pages 132-40
Authors Shiomi D, Okumura H, Homma M, Kawagishi I
Title The aspartate chemoreceptor Tar is effectively methylated by binding to the methyltransferase mainly through hydrophobic interaction.
Related PDB
Related UniProtKB
[10]
Resource
Comments
Medline ID
PubMed ID 11435446
Journal J Biol Chem
Year 2001
Volume 276
Pages 32984-9
Authors Barnakov AN, Barnakova LA, Hazelbauer GL
Title Location of the receptor-interaction site on CheB, the methylesterase response regulator of bacterial chemotaxis.
Related PDB
Related UniProtKB
[11]
Resource
Comments
Medline ID
PubMed ID 11243808
Journal J Mol Biol
Year 2001
Volume 307
Pages 119-35
Authors Kim C, Jackson M, Lux R, Khan S
Title Determinants of chemotactic signal amplification in Escherichia coli.
Related PDB
Related UniProtKB
[12]
Resource
Comments
Medline ID
PubMed ID 12022879
Journal Biochemistry
Year 2002
Volume 41
Pages 6752-60
Authors Anand GS, Stock AM
Title Kinetic basis for the stimulatory effect of phosphorylation on the methylesterase activity of CheB.
Related PDB
Related UniProtKB
[13]
Resource
Comments
Medline ID
PubMed ID 12123464
Journal Mol Microbiol
Year 2002
Volume 45
Pages 555-68
Authors Zimmer MA, Szurmant H, Saulmon MM, Collins MA, Bant JS, Ordal GW
Title The role of heterologous receptors in McpB-mediated signalling in Bacillus subtilis chemotaxis.
Related PDB
Related UniProtKB

Comments

Created Updated
2004-03-25 2009-02-26