DB code: D00143
| CATH domain | 1.20.90.10 : Phospholipase A2 | Catalytic domain |
|---|---|---|
| 4.10.410.10 : Factor Xa Inhibitor | ||
| E.C. | 3.1.1.4 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Pfam |
|---|---|---|---|
| P00617 |
Phospholipase A2, beta bungarotoxin A1 chain
|
EC
3.1.1.4
Phosphatidylcholine 2-acylhydrolase |
PF00068
(Phospholip_A2_1)
[Graphical View] |
| P00989 |
Beta-bungarotoxin B2 chain
|
None |
PF00014
(Kunitz_BPTI)
[Graphical View] |
| KEGG enzyme name |
|---|
|
phospholipase A2
lecithinase A phosphatidase phosphatidolipase phospholipase A |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P00617 | PA21B_BUNMU | Phosphatidylcholine + H(2)O = 1- acylglycerophosphocholine + a carboxylate. | Heterodimer, disulfide-linked. The A chains have phospholipase A2 activity and the B chains show homology with the basic protease inhibitors. The A1 chain is found in beta-1 and beta-2 bungarotoxins. | Secreted. | Binds 1 calcium ion (By similarity). |
| P00989 | IVB2_BUNMU | Heterodimer, disulfide-linked. The A chains have phospholipase A2 activity and the B chains show homology with the basic protease inhibitors. | Secreted. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00564 | Glycerophospholipid metabolism | |
| MAP00565 | Ether lipid metabolism | |
| MAP00590 | Arachidonic acid metabolism | |
| MAP00591 | Linoleic acid metabolism | |
| MAP00592 | alpha-Linolenic acid metabolism |
| Compound table | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||
| KEGG-id | C00076 | C00157 | C00001 | C00060 | C04230 | ||||||
| E.C. | |||||||||||
| Compound | Calcium | phosphatidylcholine | H2O | Carboxylate | 1-Acyl-sn-glycero-3-phosphocholine | ||||||
| Type | divalent metal (Ca2+, Mg2+) | amine group,carbohydrate,lipid,phosphate group/phosphate ion | H2O | carboxyl group | amine group,carbohydrate,lipid,phosphate group/phosphate ion | ||||||
| ChEBI |
29108 29108 |
15377 15377 |
|||||||||
| PubChem |
271 271 |
22247451 962 22247451 962 |
|||||||||
| 1bunA |
|
|
|
|
|
Analogue:_NA_87 | Unbound | Unbound | Unbound | ||
| 1bunB |
|
|
|
|
|
Unbound | Unbound | Unbound | Unbound | ||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;P00617 | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1bunA |
|
|
|
|
|
HIS 48;ASP 94 | TYR 28;GLY 30;GLY 32;ASP 49(Calcium binding) | |||
| 1bunB |
|
|
|
|
|
|||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | CHARACTERIZATION OF PRESYNAPTIC NEUROTOXINS. |
| Medline ID | 78043174 |
| PubMed ID | 303565 |
| Journal | Eur J Biochem |
| Year | 1977 |
| Volume | 80 |
| Pages | 1-12 |
| Authors | Abe T, Alema S, Miledi R |
| Title | Isolation and characterization of presynaptically acting neurotoxins from the venom of Bungarus snakes. |
| Related PDB | |
| Related UniProtKB | P00617 |
| [2] | |
| Resource | |
| Comments | CHARACTERIZATION OF PHOSPHOLIPASE A2 ACTIVITY. |
| Medline ID | 79088714 |
| PubMed ID | 730754 |
| Journal | J Biochem (Tokyo) |
| Year | 1978 |
| Volume | 84 |
| Pages | 1301-8 |
| Authors | Kondo K, Toda H, Narita K |
| Title |
Characterization of phospholipase A activity of beta1-bungarotoxin from Bungarus multicinctus venom. |
| Related PDB | |
| Related UniProtKB | P00617 |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2590165 |
| Journal | Biochem J |
| Year | 1989 |
| Volume | 262 |
| Pages | 773-9 |
| Authors | Chu ST, Chen YH |
| Title | The intrinsic tryptophan fluorescence of beta 1-bungarotoxin and the Ca2+-binding domains of the toxin as probed with Tb3+ luminescence. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1898340 |
| Journal | Biochem J |
| Year | 1991 |
| Volume | 278 |
| Pages | 481-6 |
| Authors | Chu ST, Chen YH |
| Title | Role of the N-terminal region of phospholipase A2 subunit of beta 1-bungarotoxin in the toxin-Ca2+ complex-formation. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 1485347 |
| Journal | Toxicon |
| Year | 1992 |
| Volume | 30 |
| Pages | 1501-4 |
| Authors | Shina R, Rosenberg P, Condrea E |
| Title | An EDTA.Ca2+ complex inhibits the enzymatic activity but not the lethality of beta-bungarotoxin. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8240282 |
| Journal | Biochem J |
| Year | 1993 |
| Volume | 295 |
| Pages | 713-8 |
| Authors | Chu ST, Chu CC, Tseng CC, Chen YH |
| Title | Met-8 of the beta 1-bungarotoxin phospholipase A2 subunit is essential for the phospholipase A2-independent neurotoxic effect. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7702746 |
| Journal | J Protein Chem |
| Year | 1994 |
| Volume | 13 |
| Pages | 641-8 |
| Authors | Chang LS, Kuo KW, Lin SR, Chang CC |
| Title | Functional involvement of Lys-6 in the enzymatic activity of phospholipase A2 from Bungarus multicinctus (Taiwan banded krait) snake venom. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8060495 |
| Journal | J Protein Chem |
| Year | 1994 |
| Volume | 13 |
| Pages | 233-6 |
| Authors | Chang LS, Lin SR, Chang CC, Yang CC |
| Title | The essentiality of B chain in stabilizing the structure of the A chain in beta 1-bungarotoxin from Bungarus multicinctus venom. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 8590005 |
| Journal | Structure |
| Year | 1995 |
| Volume | 3 |
| Pages | 1109-19 |
| Authors | Kwong PD, McDonald NQ, Sigler PB, Hendrickson WA |
| Title | Structure of beta 2-bungarotoxin: potassium channel binding by Kunitz modules and targeted phospholipase action. |
| Related PDB | 1bun |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8829622 |
| Journal | Biochem Mol Biol Int |
| Year | 1996 |
| Volume | 38 |
| Pages | 617-23 |
| Authors | Chang LS, Wen EY, Chang CC |
| Title | The structural elements of phospholipase A2 affecting the enhancement of 8-anilinonaphthalene-1-sulfonate fluorescence. |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9161720 |
| Journal | Biochem Mol Biol Int |
| Year | 1997 |
| Volume | 41 |
| Pages | 1247-53 |
| Authors | Chang LS, Chang CC, Wu PF |
| Title | The structural and functional essentiality of the N-terminal alpha-helix in the phospholipase A2 of the Taiwan banded krait. |
| Related PDB | |
| Related UniProtKB | |
| [12] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11286555 |
| Journal | J Mol Biol |
| Year | 2001 |
| Volume | 307 |
| Pages | 1049-59 |
| Authors | Singh G, Gourinath S, Sharma S, Paramasivam M, Srinivasan A, Singh TP |
| Title | Sequence and crystal structure determination of a basic phospholipase A2 from common krait (Bungarus caeruleus) at 2.4 A resolution: identification and characterization of its pharmacological sites. |
| Related PDB | |
| Related UniProtKB | |
| [13] | |
| Resource | |
| Comments | REVIEW. |
| Medline ID | 20396379 |
| PubMed ID | 10936627 |
| Journal | Toxicon |
| Year | 2001 |
| Volume | 39 |
| Pages | 107-18 |
| Authors | Rowan EG |
| Title | What does beta-bungarotoxin do at the neuromuscular junction? |
| Related PDB | |
| Related UniProtKB | P00617 P00989 |
| Comments |
|---|
|
This protein, |
| Created | Updated |
|---|---|
| 2004-11-19 | 2009-02-26 |