DB code: D00131
| CATH domain | 3.40.50.2020 : Rossmann fold | Catalytic domain |
|---|---|---|
| 3.40.50.2020 : Rossmann fold | Catalytic domain | |
| E.C. | 2.7.6.1 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.50.2020 : Rossmann fold | S00288 S00289 S00287 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | RefSeq | Pfam |
|---|---|---|---|---|
| P14193 |
Ribose-phosphate pyrophosphokinase
|
RPPK
EC 2.7.6.1 Phosphoribosyl pyrophosphate synthetase P-Rib-PP synthetase PRPP synthetase |
NP_387932.1
(Protein)
NC_000964.3 (DNA/RNA sequence) |
PF00156
(Pribosyltran)
[Graphical View] |
| KEGG enzyme name |
|---|
|
ribose-phosphate diphosphokinase
ribose-phosphate pyrophosphokinase PRPP synthetase phosphoribosylpyrophosphate synthetase PPRibP synthetase PP-ribose P synthetase 5-phosphoribosyl-1-pyrophosphate synthetase 5-phosphoribose pyrophosphorylase 5-phosphoribosyl-alpha-1-pyrophosphate synthetase phosphoribosyl-diphosphate synthetase phosphoribosylpyrophosphate synthase pyrophosphoribosylphosphate synthetase ribophosphate pyrophosphokinase ribose-5-phosphate pyrophosphokinase |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| P14193 | KPRS_BACSU | ATP + D-ribose 5-phosphate = AMP + 5-phospho- alpha-D-ribose 1-diphosphate. | Homohexamer. | Cytoplasm (By similarity). | Binds 1 magnesium ion per subunit. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00030 | Pentose phosphate pathway | |
| MAP00230 | Purine metabolism |
| Compound table | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cofactors | Substrates | Products | Intermediates | ||||||||||
| KEGG-id | C00305 | C00002 | C00131 | C00117 | C00020 | C00360 | C00119 | ||||||
| E.C. | |||||||||||||
| Compound | Magnesium | ATP | dATP | D-Ribose 5-phosphate | AMP | dAMP | 5-Phospho-alpha-D-ribose 1-diphosphate | ||||||
| Type | divalent metal (Ca2+, Mg2+) | amine group,nucleotide | amine group,nucleotide | carbohydrate,phosphate group/phosphate ion | amine group,nucleotide | amine group,nucleotide | carbohydrate,phosphate group/phosphate ion | ||||||
| ChEBI |
18420 18420 |
15422 15422 |
16284 16284 |
52742 52742 |
16027 16027 |
17713 17713 |
17111 17111 |
||||||
| PubChem |
888 888 |
5957 5957 |
15993 15993 |
439167 439167 |
6083 6083 |
12599 12599 |
7339 7339 |
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| 1dkrA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dkrB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dkuA01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dkuB01 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ibsA01 |
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Analogue:_CD | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1ibsB01 |
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Analogue:_CD | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dkrA02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dkrB02 |
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Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | Unbound | |
| 1dkuA02 |
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Unbound | Analogue:AP2 | Unbound | Unbound | Analogue:ABM | Unbound | Unbound | |
| 1dkuB02 |
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Unbound | Analogue:AP2 | Unbound | Unbound | Analogue:ABM | Unbound | Unbound | |
| 1ibsA02 |
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Analogue:_CD | Unbound | Unbound | Unbound | Analogue:ABM | Unbound | Unbound | |
| 1ibsB02 |
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Analogue:_CD | Unbound | Unbound | Unbound | Analogue:ABM | Unbound | Unbound | |
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1dkrA01 |
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LYS 197 | ||||
| 1dkrB01 |
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LYS 197 | ||||
| 1dkuA01 |
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LYS 197 | ||||
| 1dkuB01 |
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LYS 197 | ||||
| 1ibsA01 |
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LYS 197 | ||||
| 1ibsB01 |
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LYS 197 | ||||
| 1dkrA02 |
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ARG 101 | ASP 133;HIS 135;ASP 144;ASP 148(magnesium binding) | |||
| 1dkrB02 |
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ARG 101 | ASP 133;HIS 135;ASP 144;ASP 148(magnesium binding) | |||
| 1dkuA02 |
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ARG 101 | ASP 133;HIS 135;ASP 144;ASP 148(magnesium binding) | |||
| 1dkuB02 |
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ARG 101 | ASP 133;HIS 135;ASP 144;ASP 148(magnesium binding) | |||
| 1ibsA02 |
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ARG 101 | ASP 133;HIS 135;ASP 144;ASP 148(magnesium binding) | |||
| 1ibsB02 |
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ARG 101 | ASP 133;HIS 135;ASP 144;ASP 148(magnesium binding) | |||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
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[7]
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p.305-306 | |
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[8]
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p.737 | |
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[10]
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p.2319-2320 | |
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[11]
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p.277 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 6330252 |
| Journal | J Lab Clin Med |
| Year | 1984 |
| Volume | 104 |
| Pages | 96-109 |
| Authors | Itkin P, Woo S, Becker MA |
| Title | Human phosphoribosylpyrophosphate synthetase: radioimmunochemical quantitation in erythrocytes and fibroblasts. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2414323 |
| Journal | J Clin Invest |
| Year | 1985 |
| Volume | 76 |
| Pages | 1657-64 |
| Authors | Losman MJ, Rimon D, Kim M, Becker MA |
| Title | Selective expression of phosphoribosylpyrophosphate synthetase superactivity in human lymphoblast lines. |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 3017368 |
| Journal | Arthritis Rheum |
| Year | 1986 |
| Volume | 29 |
| Pages | 880-8 |
| Authors | Becker MA, Losman MJ, Rosenberg AL, Mehlman I, Levinson DJ, Holmes EW |
| Title |
Phosphoribosylpyrophosphate synthetase superactivity. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 2546925 |
| Journal | J Biochem (Tokyo) |
| Year | 1989 |
| Volume | 105 |
| Pages | 736-41 |
| Authors | Kita K, Otsuki T, Ishizuka T, Tatibana M |
| Title | Rat liver phosphoribosyl pyrophosphate synthetase: existence of the purified enzyme as heterogeneous aggregates and identification of the catalytic subunit. |
| Related PDB | |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 8820490 |
| Journal | Proteins |
| Year | 1996 |
| Volume | 24 |
| Pages | 238-46 |
| Authors | Bentsen AK, Larsen TA, Kadziola A, Larsen S, Harlow KW |
| Title | Overexpression of Bacillus subtilis phosphoribosylpyrophosphate synthetase and crystallization and preliminary X-ray characterization of the free enzyme and its substrate-effector complexes. |
| Related PDB | 1kdr |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9348095 |
| Journal | J Biochem (Tokyo) |
| Year | 1997 |
| Volume | 122 |
| Pages | 635-40 |
| Authors | Sonoda T, Kita K, Ishijima S, Ishizuka T, Ahmad I, Tatibana M |
| Title | Kinetic and regulatory properties of rat liver phosphoribosylpyrophosphate synthetase complex are partly distinct from those of isolated recombinant component catalytic subunits. |
| Related PDB | |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) |
| Medline ID | 20207047 |
| PubMed ID | 10742175 |
| Journal | Nat Struct Biol |
| Year | 2000 |
| Volume | 7 |
| Pages | 303-8 |
| Authors | Eriksen TA, Kadziola A, Bentsen AK, Harlow KW, Larsen S |
| Title | Structural basis for the function of Bacillus subtilis phosphoribosyl-pyrophosphate synthetase. |
| Related PDB | 1dkr 1dku |
| Related UniProtKB | P14193 |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11751055 |
| Journal | Curr Opin Struct Biol |
| Year | 2001 |
| Volume | 11 |
| Pages | 733-9 |
| Authors | Sinha SC, Smith JL |
| Title | The PRT protein family. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11278632 |
| Journal | J Biol Chem |
| Year | 2001 |
| Volume | 276 |
| Pages | 17851-6 |
| Authors | Krath BN, Hove-Jensen B |
| Title |
Class II recombinant phosphoribosyl diphosphate synthase from spinach. |
| Related PDB | |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11604537 |
| Journal | Protein Sci |
| Year | 2001 |
| Volume | 10 |
| Pages | 2317-24 |
| Authors | Krath BN, Hove-Jensen B |
| Title |
Implications of secondary structure prediction and amino acid sequence comparison of class I and class II phosphoribosyl diphosphate synthases on catalysis, |
| Related PDB | |
| Related UniProtKB | |
| [11] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11790837 |
| Journal | Protein Sci |
| Year | 2002 |
| Volume | 11 |
| Pages | 271-9 |
| Authors | Eriksen TA, Kadziola A, Larsen S |
| Title | Binding of cations in Bacillus subtilis phosphoribosyldiphosphate synthetase and their role in catalysis. |
| Related PDB | |
| Related UniProtKB | |
| Comments |
|---|
| Created | Updated |
|---|---|
| 2004-03-18 | 2009-02-26 |