DB code: D00091

CATH domain	3.30.70.250 : Alpha-Beta Plaits
CATH domain	3.40.366.10 : Malonyl-Coenzyme A Acyl Carrier Protein; domain 2	Catalytic domain
E.C.	2.3.1.39
CSA	1mla
M-CSA	1mla
MACiE	M0291

CATH domain	Related DB codes (homologues)

Uniprot Enzyme Name
UniprotKB	Protein name	Synonyms	RefSeq	Pfam
P0AAI9	Malonyl CoA-acyl carrier protein transacylase	MCT EC 2.3.1.39	NP_415610.1 (Protein) NC_000913.2 (DNA/RNA sequence) YP_489360.1 (Protein) NC_007779.1 (DNA/RNA sequence)	PF00698 (Acyl_transf_1) [Graphical View]

KEGG enzyme name
[acyl-carrier-protein] S-malonyltransferase [acyl carrier protein]malonyltransferase FabD malonyl coenzyme A-acyl carrier protein transacylase malonyl transacylase malonyl transferase malonyl-CoA-acyl carrier protein transacylase malonyl-CoA:[acyl-carrier-protein] S-malonyltransferase malonyl-CoA:ACP transacylase malonyl-CoA:ACP-SH transacylase malonyl-CoA:AcpM transacylase malonyl-CoA:acyl carrier protein transacylase malonyl-CoA:acyl-carrier-protein transacylase malonyl-CoA/dephospho-CoA acyltransferase MAT MCAT MdcH

KEGG enzyme name

[acyl-carrier-protein] S-malonyltransferase
[acyl carrier protein]malonyltransferase
FabD
malonyl coenzyme A-acyl carrier protein transacylase
malonyl transacylase
malonyl transferase
malonyl-CoA-acyl carrier protein transacylase
malonyl-CoA:[acyl-carrier-protein] S-malonyltransferase
malonyl-CoA:ACP transacylase
malonyl-CoA:ACP-SH transacylase
malonyl-CoA:AcpM transacylase
malonyl-CoA:acyl carrier protein transacylase
malonyl-CoA:acyl-carrier-protein transacylase
malonyl-CoA/dephospho-CoA acyltransferase
MAT
MCAT
MdcH

UniprotKB: Accession Number	Entry name	Activity	Subunit	Subcellular location	Cofactor
P0AAI9	FABD_ECOLI	Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].

KEGG Pathways
Map code	Pathways	E.C.
MAP00061	Fatty acid biosynthesis

Compound table
	Substrates		Products		Intermediates
KEGG-id	C00083	C00229	C00010	C01209
E.C.
Compound	Malonyl-CoA	Acyl-carrier protein	CoA	Malonyl-[acyl-carrier protein]
Type	amine group,carbohydrate,carboxyl group,nucleotide ,peptide/protein,sulfide group	carbohydrate,peptide/protein,phosphate group/phosphate ion,sulfhydryl group	amine group,carbohydrate,nucleotide ,peptide/protein,sulfhydryl group	carbohydrate,carboxyl group,peptide/protein,phosphate group/phosphate ion,sulfide group
ChEBI	15531 15531		15346 15346
PubChem	10663 644066 10663 644066		6816 87642 6816 87642

1mlaA01	Unbound	Unbound	Unbound	Unbound
1mlaA02	Unbound	Unbound	Unbound	Unbound

Reference for Active-site residues
resource	references	E.C.
PDB;1mla & Swiss-prot;P0AAI9

Active-site residues
PDB	Catalytic residues	Cofactor-binding residues	Modified residues	Main-chain involved in catalysis	Comment

1mlaA01
1mlaA02	SER 92;HIS 201

References for Catalytic Mechanism
References	Sections	No. of steps in catalysis
[2]	p.12962-12964

References
[1]
Resource
Comments
Medline ID
PubMed ID	8078074
Journal	J Mol Biol
Year	1994
Volume	242
Pages	99-102
Authors	Serre L, Swenson L, Green R, Wei Y, Verwoert II, Verbree EC, Stuitje AR, Derewenda ZS
Title	Crystallization of the malonyl coenzyme A-acyl carrier protein transacylase from Escherichia coli.
Related PDB
Related UniProtKB
[2]
Resource
Comments	X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS)
Medline ID	95286570
PubMed ID	7768883
Journal	J Biol Chem
Year	1995
Volume	270
Pages	12961-4
Authors	Serre L, Verbree EC, Dauter Z, Stuitje AR, Derewenda ZS
Title	The Escherichia coli malonyl-CoA:acyl carrier protein transacylase at 1.5-A resolution. Crystal structure of a fatty acid synthase component.
Related PDB	1mla
Related UniProtKB	P0AAI9
[3]
Resource
Comments
Medline ID
PubMed ID	9215574
Journal	Protein Eng
Year	1997
Volume	10
Pages	561-6
Authors	Rangan VS, Serre L, Witkowska HE, Bari A, Smith S
Title	Characterization of the malonyl-/acetyltransacylase domain of the multifunctional animal fatty acid synthase by expression in Escherichia coli and refolding in vitro.
Related PDB
Related UniProtKB
[4]
Resource
Comments
Medline ID
PubMed ID	9862793
Journal	Chem Biol
Year	1998
Volume	5
Pages	699-711
Authors	Matharu AL, Cox RJ, Crosby J, Byrom KJ, Simpson TJ
Title	MCAT is not required for in vitro polyketide synthesis in a minimal actinorhodin polyketide synthase from Streptomyces coelicolor.
Related PDB
Related UniProtKB
[5]
Resource
Comments
Medline ID
PubMed ID	9632638
Journal	J Biol Chem
Year	1998
Volume	273
Pages	15920-6
Authors	Wilson MC, Jackson VN, Heddle C, Price NT, Pilegaard H, Juel C, Bonen A, Montgomery I, Hutter OF, Halestrap AP
Title	Lactic acid efflux from white skeletal muscle is catalyzed by the monocarboxylate transporter isoform MCT3.
Related PDB
Related UniProtKB
[6]
Resource
Comments
Medline ID
PubMed ID	10421763
Journal	Chem Biol
Year	1999
Volume	6
Pages	577-84
Authors	Zhou P, Florova G, Reynolds KA
Title	Polyketide synthase acyl carrier protein (ACP) as a substrate and a catalyst for malonyl ACP biosynthesis.
Related PDB
Related UniProtKB
[7]
Resource
Comments
Medline ID
PubMed ID	10684659
Journal	Biochemistry
Year	2000
Volume	39
Pages	2088-95
Authors	Dreier J, Khosla C
Title	Mechanistic analysis of a type II polyketide synthase. Role of conserved residues in the beta-ketoacyl synthase-chain length factor heterodimer.
Related PDB
Related UniProtKB
[8]
Resource
Comments
Medline ID
PubMed ID	11814333
Journal	Biochemistry
Year	2002
Volume	41
Pages	1421-7
Authors	Szafranska AE, Hitchman TS, Cox RJ, Crosby J, Simpson TJ
Title	Kinetic and mechanistic analysis of the malonyl CoA:ACP transacylase from Streptomyces coelicolor indicates a single catalytically competent serine nucleophile at the active site.
Related PDB
Related UniProtKB

Comments

Created	Updated
2004-03-17	2012-06-01