DB code: D00022
| CATH domain | 3.40.605.10 : Aldehyde Dehydrogenase; Chain A, domain 1 | Catalytic domain |
|---|---|---|
| 3.40.309.10 : Aldehyde Dehydrogenase; Chain A, domain 2 | Catalytic domain | |
| E.C. | 1.2.1.9 | |
| CSA | ||
| M-CSA | ||
| MACiE | ||
| CATH domain | Related DB codes (homologues) |
|---|---|
| 3.40.309.10 : Aldehyde Dehydrogenase; Chain A, domain 2 | D00020 D00021 D00475 D00614 |
| 3.40.605.10 : Aldehyde Dehydrogenase; Chain A, domain 1 | D00020 D00021 D00475 D00614 |
| Uniprot Enzyme Name | UniprotKB | Protein name | Synonyms | Pfam | RefSeq |
|---|---|---|---|---|
| O57693 |
|
Glyceraldehyde-3-phosphate dehydrogenase (NADP+)
EC 1.2.1.9 |
PF00171
(Aldedh)
[Graphical View] |
|
| Q59931 |
NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
|
EC
1.2.1.9
Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase Glyceraldehyde-3-phosphate dehydrogenase [NADP+] Triosephosphate dehydrogenase |
PF00171
(Aldedh)
[Graphical View] |
NP_721104.1
(Protein)
NC_004350.2 (DNA/RNA sequence) |
| KEGG enzyme name |
|---|
|
glyceraldehyde-3-phosphate dehydrogenase (NADP+)
triosephosphate dehydrogenase dehydrogenase, glyceraldehyde phosphate (nicotinamide adeninedinucleotide phosphate) glyceraldehyde phosphate dehydrogenase (NADP+) glyceraldehyde 3-phosphate dehydrogenase (NADP+) NADP+-glyceraldehyde phosphate dehydrogenase NADP+-glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate:NADP+ reductase nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase (NADP+) |
| UniprotKB: Accession Number | Entry name | Activity | Subunit | Subcellular location | Cofactor |
|---|---|---|---|---|---|
| O57693 | O57693_THETE | ||||
| Q59931 | GAPN_STRMU | D-glyceraldehyde 3-phosphate + NADP(+) + H(2)O = 3-phospho-D-glycerate + NADPH. | Homotetramer. |
| KEGG Pathways | Map code | Pathways | E.C. |
|---|---|---|
| MAP00010 | Glycolysis / Gluconeogenesis |
| Compound table | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Substrates | Products | Intermediates | ||||||||||
| KEGG-id | C00118 | C00006 | C00001 | C00197 | C00005 | C00080 | ||||||
| E.C. | ||||||||||||
| Compound | D-Glyceraldehyde 3-phosphate | NADP+ | H2O | 3-Phospho-D-glycerate | NADPH | H+ | ||||||
| Type | carbohydrate,phosphate group/phosphate ion | amide group,amine group,nucleotide | H2O | carbohydrate,carboxyl group,phosphate group/phosphate ion | amide group,amine group,nucleotide | others | ||||||
| ChEBI |
29052 29052 |
18009 18009 |
15377 15377 |
17794 17794 |
16474 16474 |
15378 15378 |
||||||
| PubChem |
439168 439168 |
5886 5886 |
22247451 962 22247451 962 |
439183 439183 |
5884 5884 |
1038 1038 |
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| 1ky8A01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1uxnA01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1uxpA01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1uxqA01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1uxrA01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1uxtA01 |
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Unbound | Analogue:NAD | Unbound | Unbound | |||
| 1uxuA01 |
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Bound:G3H | Analogue:NAP | Unbound | Unbound | |||
| 1uxvA01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1euhA01 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1euhB01 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1euhC01 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1euhD01 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1qi1A01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1qi1B01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1qi1C01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1qi1D01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1qi6A01 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1qi6B01 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1qi6C01 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1qi6D01 |
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Unbound | Unbound | Unbound | Unbound | |||
| 2euhA01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 2euhB01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 2euhC01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 2euhD01 |
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Unbound | Bound:NAP | Unbound | Unbound | |||
| 1ky8A02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1uxnA02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1uxpA02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1uxqA02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1uxrA02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1uxtA02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1uxuA02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1uxvA02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1euhA02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1euhB02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1euhC02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1euhD02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1qi1A02 |
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Bound:G3H | Unbound | Unbound | Unbound | |||
| 1qi1B02 |
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Bound:G3H | Unbound | Unbound | Unbound | |||
| 1qi1C02 |
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Bound:G3H | Unbound | Unbound | Unbound | |||
| 1qi1D02 |
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Bound:G3H | Unbound | Unbound | Unbound | |||
| 1qi6A02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1qi6B02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1qi6C02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 1qi6D02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 2euhA02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 2euhB02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 2euhC02 |
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Unbound | Unbound | Unbound | Unbound | |||
| 2euhD02 |
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Unbound | Unbound | Unbound | Unbound | |||
| Reference for Active-site residues | ||
|---|---|---|
| resource | references | E.C. |
| Swiss-prot;Q59931 & literature [5], [6], [7] & [10] | ||
| Active-site residues | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PDB | Catalytic residues | Cofactor-binding residues | Modified residues | Main-chain involved in catalysis | Comment | |||||
| 1ky8A01 |
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ASN 168;GLU 263 | ||||
| 1uxnA01 |
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ASN 168;GLU 263 | ||||
| 1uxpA01 |
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ASN 168;GLU 263 | ||||
| 1uxqA01 |
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ASN 168;GLU 263 | ||||
| 1uxrA01 |
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ASN 168;GLU 263 | ||||
| 1uxtA01 |
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ASN 168;GLU 263 | ||||
| 1uxuA01 |
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ASN 168;GLU 263 | ||||
| 1uxvA01 |
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ASN 168;GLU 263 | ||||
| 1euhA01 |
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ASN 154;GLU 250 | ||||
| 1euhB01 |
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ASN 154;GLU 250 | ||||
| 1euhC01 |
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ASN 154;GLU 250 | ||||
| 1euhD01 |
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ASN 154;GLU 250 | ||||
| 1qi1A01 |
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ASN 154;GLU 250 | ||||
| 1qi1B01 |
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ASN 154;GLU 250 | ||||
| 1qi1C01 |
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ASN 154;GLU 250 | ||||
| 1qi1D01 |
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ASN 154;GLU 250 | ||||
| 1qi6A01 |
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ASN 154;GLU 250 | ||||
| 1qi6B01 |
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ASN 154;GLU 250 | ||||
| 1qi6C01 |
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ASN 154;GLU 250 | ||||
| 1qi6D01 |
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ASN 154;GLU 250 | ||||
| 2euhA01 |
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ASN 154;GLU 250 | ||||
| 2euhB01 |
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ASN 154;GLU 250 | ||||
| 2euhC01 |
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ASN 154;GLU 250 | ||||
| 2euhD01 |
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ASN 154;GLU 250 | ||||
| 1ky8A02 |
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CYS 297 | ||||
| 1uxnA02 |
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CYS 297 | ||||
| 1uxpA02 |
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CYS 297 | ||||
| 1uxqA02 |
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CYS 297 | ||||
| 1uxrA02 |
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CYS 297 | ||||
| 1uxtA02 |
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CYS 297 | ||||
| 1uxuA02 |
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CYS 297 | ||||
| 1uxvA02 |
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CYS 297 | ||||
| 1euhA02 |
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CYS 284 | ||||
| 1euhB02 |
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CYS 284 | ||||
| 1euhC02 |
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CYS 284 | ||||
| 1euhD02 |
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CYS 284 | ||||
| 1qi1A02 |
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mutant C284S | ||||
| 1qi1B02 |
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mutant C284S | ||||
| 1qi1C02 |
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mutant C284S | ||||
| 1qi1D02 |
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mutant C284S | ||||
| 1qi6A02 |
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CYS 284 | ||||
| 1qi6B02 |
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CYS 284 | ||||
| 1qi6C02 |
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CYS 284 | ||||
| 1qi6D02 |
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CYS 284 | ||||
| 2euhA02 |
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CYS 284 | ||||
| 2euhB02 |
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CYS 284 | ||||
| 2euhC02 |
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CYS 284 | ||||
| 2euhD02 |
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CYS 284 | ||||
| References for Catalytic Mechanism | ||
|---|---|---|
| References | Sections | No. of steps in catalysis |
|
[1]
|
p.167-168 | |
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[2]
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p.1416 | |
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[3]
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Fig.1, p.12956-12957 | |
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[5]
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Fig.6, p.3333-3334 | |
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[6]
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p.145-147 | |
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[7]
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p.17-22 | |
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[9]
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p.19942 | |
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[10]
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p.824-825 | |
| References | |
|---|---|
| [1] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 7545914 |
| Journal | J Mol Biol |
| Year | 1994 |
| Volume | 237 |
| Pages | 165-71 |
| Authors | Habenicht A, Hellman U, Cerff R |
| Title | Non-phosphorylating GAPDH of higher plants is a member of the aldehyde dehydrogenase superfamily with no sequence homology to phosphorylating GAPDH. |
| Related PDB | |
| Related UniProtKB | |
| [2] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 9461340 |
| Journal | Biol Chem |
| Year | 1997 |
| Volume | 378 |
| Pages | 1413-9 |
| Authors | Habenicht A |
| Title |
The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase: biochemistry, |
| Related PDB | |
| Related UniProtKB | |
| [3] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10504267 |
| Journal | Biochemistry |
| Year | 1999 |
| Volume | 38 |
| Pages | 12950-8 |
| Authors | Marchal S, Branlant G |
| Title | Evidence for the chemical activation of essential cys-302 upon cofactor binding to nonphosphorylating glyceraldehyde 3-phosphate dehydrogenase from Streptococcus mutans. |
| Related PDB | |
| Related UniProtKB | |
| [4] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10388564 |
| Journal | J Mol Biol |
| Year | 1999 |
| Volume | 290 |
| Pages | 161-73 |
| Authors | Cobessi D, Tete-Favier F, Marchal S, Azza S, Branlant G, Aubry A |
| Title | Apo and holo crystal structures of an NADP-dependent aldehyde dehydrogenase from Streptococcus mutans. |
| Related PDB | 1euh 2euh |
| Related UniProtKB | |
| [5] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 10727225 |
| Journal | Biochemistry |
| Year | 2000 |
| Volume | 39 |
| Pages | 3327-35 |
| Authors | Marchal S, Rahuel-Clermont S, Branlant G |
| Title | Role of glutamate-268 in the catalytic mechanism of nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans. |
| Related PDB | |
| Related UniProtKB | |
| [6] | |
| Resource | |
| Comments | X-ray crystallography |
| Medline ID | |
| PubMed ID | 10864505 |
| Journal | J Mol Biol |
| Year | 2000 |
| Volume | 300 |
| Pages | 141-52 |
| Authors | Cobessi D, Tete-Favier F, Marchal S, Branlant G, Aubry A |
| Title | Structural and biochemical investigations of the catalytic mechanism of an NADP-dependent aldehyde dehydrogenase from Streptococcus mutans. |
| Related PDB | 1qi1 1qi6 |
| Related UniProtKB | |
| [7] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11306027 |
| Journal | Chem Biol Interact |
| Year | 2001 |
| Volume | 130-132 |
| Pages | 15-28 |
| Authors | Marchal S, Cobessi D, Rahuel-Clermont S, Tete-Favier F, Aubry A, Branlant G |
| Title | Chemical mechanism and substrate binding sites of NADP-dependent aldehyde dehydrogenase from Streptococcus mutans. |
| Related PDB | |
| Related UniProtKB | |
| [8] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 12163495 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 39235-42 |
| Authors | Marchal S, Branlant G |
| Title | Characterization of the amino acids involved in substrate specificity of nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans. |
| Related PDB | |
| Related UniProtKB | |
| [9] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 11842090 |
| Journal | J Biol Chem |
| Year | 2002 |
| Volume | 277 |
| Pages | 19938-45 |
| Authors | Pohl E, Brunner N, Wilmanns M, Hensel R |
| Title | The crystal structure of the allosteric non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeum Thermoproteus tenax. |
| Related PDB | 1ky8 |
| Related UniProtKB | |
| [10] | |
| Resource | |
| Comments | |
| Medline ID | |
| PubMed ID | 15288789 |
| Journal | J Mol Biol |
| Year | 2004 |
| Volume | 341 |
| Pages | 815-28 |
| Authors | Lorentzen E, Hensel R, Knura T, Ahmed H, Pohl E |
| Title | Structural Basis of allosteric regulation and substrate specificity of the non-phosphorylating glyceraldehyde 3-Phosphate dehydrogenase from Thermoproteus tenax. |
| Related PDB | 1uxn 1uxp 1uxq 1uxr 1uxt 1uxu 1uxv |
| Related UniProtKB | |
| Comments |
|---|
|
This enzyme catalyzed complex reactions, (A) Addition of nucleophilic Cys to carbonyl group of substrate (B) Hydride transfer (C) Hydrolysis |
| Created | Updated |
|---|---|
| 2004-03-16 | 2009-03-30 |