RLCP 1.32.-.- :EzCatDB EzCatDB

R L C P
Hierarchic Classification of Catalytic Mechanisms


R 1.-.-.- : Hydrolysis (bond cleavage by water) (Reaction)

L 1.32.-.- : C-O bond (O-glycoside bond) close to N-acetyl group (Ligand group involved)

Catalytic mechanisms

  • 1.32.5000.- : Chitinase-A-like mechanism (Acid-Base/Stabilizer/Modulator); SN1-like cleavage induced by protonation to leaving group; Substrate-assisted stabilization of intermediate; water activation by general base; water attacks on intermediate

  • 1.32.60200.- : Hevamine-A-like mechanism (Acid-Base/Stabilizer); Intramolecular covalent bond within substrate induced by substrate-assisted nucleophilic attack & protonation to leaving group by general acid (SN2-like reaction); followed by activated water by base

  • 1.32.68200.- : Chitobiase-like mechanism (Acid-Base/Modulator-Stabilizer); Modulator-assisted acid protonation to leaving group leading to transition state; Modulator-assisted substrate nucleophilic attack on transition state leading to intermediate formation; Intermediate stabilization; Base-activated water attacks on intermediate leading to transition state; Bond cleavage of transision state; SN1-like reaction

  • 1.32.68230.- : Hyaluronidase-like mechanism (Acid-Base/Modulator); Modulator-assisted substrate activation; substrate-assisted nucleophilic attack forming intramolecular covalent intermediate & acid protonation to leaving group (SN1-like reaction); Base activation of water; Activated water attacks on intramolecular intermediate

    • Copyright: Nozomi Nagano, JST & CBRC-AIST
    Funded by PRESTO/Japan Science and Technology Agency (JST) (December 2001 - November 2004)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2006)
    Funded by Grant-in-Aid for Scientific Research (B)/Japan Society for the Promotion of Science (JSPS) (April 2005 - March 2008)
    Funded by BIRD/Japan Science and Technology Agency (JST) (September 2005 - September 2008)
    Funded by BIRD/Japan Science and Technology Agency (JST) (October 2007 - September 2010)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2011 - March 2012)
    Funded by Grant-in-Aid for Publication of Scientific Research Results/Japan Society for the Promotion of Science (JSPS) (April 2012 - March 2013)
    Supported by the commission for the Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI) (October 2012 - March 2016)
    Funded by the project commissioned by the New Energy and Industrial Technology Development Organization (NEDO) (April 2016 -)