Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
2,3-Dihydroxybiphenyl 1,2-Dioxygenase; domain 1
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
N-Glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase
UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I
UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I
Alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Structure of the nucleotide-diphospho-sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms.
Structure/function studies of glycosyltransferases.
X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily.
An evolving hierarchical family classification for glycosyltransferases.
Substrate-induced conformational changes in glycosyltransferases.
Catalytic mechanism of glycosyltransferases: hybrid quantum mechanical/molecular mechanical study of the inverting N-acetylglucosaminyltransferase I.
X-ray crystal structures of rabbit N-acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues.
Glycosyltransferases: structures, functions, and mechanisms.
This enzyme belongs to glycosyltransferase family-13 (GT13 family), with a GT-A fold and an inverting mechanism (see [8]). The structure of the N-terminal domain, which contains the N-terminal cytoplasmic region and a membrane-bound region, has not been determined yet. According to the literature [3], [7] and [8], this enzyme catalyzes the following reaction: (0) Manganese ion, bound to Asp213, stabilizes the negative charge on the leaving phosphate groups. (1) Asp291 acts as a general base to deprotonate O2 atom of the acceptor, mannosyl group, to activate it. (2) The activated O2 atom of the mannosyl group makes a nucleophilic attack on the C1 atom of N-acetyl-glucosamine of UDP-N-acetyl-D-glucosamine, leading to the transition state, where a positive charge is developed on the C1 atom and negative charges on the O2 atom and the leaving phosphate oxygen. (3) The bond between the C1 atom and the leaving phosphate group is cleaved while the bond between the O2 atom and the C1 atom is formed. (SN2-like reaction)