T00091
type: Enzyme
cath: Glycosyltransferase
cath: Beta-galactosidase; Chain A, domain 5
cath: Chondroitinase Ac; Chain A, domain 3
ec: https://purl.uniprot.org/enzyme/4.2.2.5
csa: 1cb8
uniprot: Q59288
kegg enzyme: chondroitin AC lyase
kegg enzyme: chondroitinase (ambiguous)
kegg enzyme: chondroitin sulfate lyase
kegg enzyme: chondroitin AC eliminase
kegg enzyme: chondroitinase AC
kegg enzyme: ChnAC
kegg cofactor: Calcium
kegg substrate: Chondroitin 4-sulfate
kegg substrate: Chondroitin 6-sulfate
kegg product: Chondroitin 4-sulfate
kegg product: 4-Deoxy-beta-D-gluc-4-enuronosyl-(1,3)-N-acetyl-D-galactosamine 4-sulfate
kegg product: Chondroitin 6-sulfate
kegg product: 4-Deoxy-beta-D-gluc-4-enuronosyl-(1,3)-N-acetyl-D-galactosamine 6-sulfate
pdb: 1cb8A01
pdb: 1hm2A01
pdb: 1hm3A01
pdb: 1hmuA01
pdb: 1hmwA01
pdb: 1cb8A02
pdb: 1hm2A02
pdb: 1hm3A02
pdb: 1hmuA02
pdb: 1hmwA02
pdb: 1cb8A03
pdb: 1hm2A03
pdb: 1hm3A03
pdb: 1hmuA03
pdb: 1hmwA03
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1cb8A01
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hm3A01
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hmuA01
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hmwA01
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1cb8A02
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hm2A02
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hm3A02
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hmuA02
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hmwA02
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1cb8A03
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hm2A03
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hm3A03
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hmuA03
pdb bound state: Unbound
  compound: Chondroitin 4-sulfate
  pdb: 1hmwA03
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1cb8A01
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hm2A01
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hm3A01
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hmuA01
pdb bound state: Analogue
  compound: Chondroitin 6-sulfate
  pdb: 1hmwA01
  ligand het code: GCD-ASG-BDP-NG6 (chain D)
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1cb8A02
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hm2A02
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hm3A02
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hmuA02
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hmwA02
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1cb8A03
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hm2A03
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hm3A03
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hmuA03
pdb bound state: Unbound
  compound: Chondroitin 6-sulfate
  pdb: 1hmwA03
pdb bound state: Unbound
  compound: Calcium
  pdb: 1cb8A01
pdb bound state: Unbound
  compound: Calcium
  pdb: 1hm2A01
pdb bound state: Unbound
  compound: Calcium
  pdb: 1hm3A01
pdb bound state: Unbound
  compound: Calcium
  pdb: 1hmuA01
pdb bound state: Unbound
  compound: Calcium
  pdb: 1hmwA01
active site residues resources: Development of an assay and determination of kinetic parameters for chondroitin AC lyase using defined synthetic substrates.
active site residues resources: Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum.
active site residues resources: Q59288
active site residues resources: 1cb8
references: Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes.
references: Active site of chondroitin AC lyase revealed by the structure of enzyme-oligosaccharide complexes and mutagenesis.
references: Development of an assay and determination of kinetic parameters for chondroitin AC lyase using defined synthetic substrates.
references: Role of arginine 292 in the catalytic activity of chondroitin AC lyase from Flavobacterium heparinum.
references: Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum.
references: Synthesis and evaluation of potential inhibitors of chondroitin AC lyase from Flavobacterium heparinum.
comment: The information on substrate & product was obtained from literature [3].

Glycosyltransferase
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/1.50.10.100
catalytic domain: index.html

Beta-galactosidase; Chain A, domain 5
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/2.70.98.10

Chondroitinase Ac; Chain A, domain 3
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/2.60.220.10

Calcium
type: KeggCofactor
references: KEGG:C00076
compound type: divalent metal (Ca2+, Mg2+)
references: CHEBI:29108
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

Chondroitin 4-sulfate
type: KeggSubstrate
references: KEGG:C00634
compound type: amide group
compound type: carbohydrate
compound type: carboxyl group
compound type: polysaccharide
compound type: sulfate group
type: KeggProduct

Chondroitin 6-sulfate
type: KeggSubstrate
references: KEGG:C00635
compound type: amide group
compound type: carbohydrate
compound type: carboxyl group
compound type: polysaccharide
compound type: sulfate group
type: KeggProduct

4-Deoxy-beta-D-gluc-4-enuronosyl-(1,3)-N-acetyl-D-galactosamine 4-sulfate
type: KeggProduct
references: KEGG:C04864
compound type: amide group
compound type: carboxyl group
compound type: polysaccharide
compound type: sulfate group
references: CHEBI:15931
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

4-Deoxy-beta-D-gluc-4-enuronosyl-(1,3)-N-acetyl-D-galactosamine 6-sulfate
type: KeggProduct
references: KEGG:C04865
compound type: amide group
compound type: carboxyl group
compound type: polysaccharide
compound type: sulfate group
references: CHEBI:16735
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

1cb8A01
type: Pdb
references: 1cb8
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1cb8
catalytic residue: HIS 225;TYR 234;ARG 288

1hm2A01
type: Pdb
references: 1hm2
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1hm2
catalytic residue: HIS 225;TYR 234;ARG 288

1hm3A01
type: Pdb
references: 1hm3
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1hm3
catalytic residue: HIS 225;TYR 234;ARG 288

1hmuA01
type: Pdb
references: 1hmu
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1hmu
catalytic residue: HIS 225;TYR 234;ARG 288

1hmwA01
type: Pdb
references: 1hmw
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1hmw
catalytic residue: HIS 225;TYR 234;ARG 288

1cb8A02
type: Pdb
references: 1cb8
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1cb8
cofactor binding residue: GLU 405;ASP 407;ASP 416;TYR 417(Calcium binding)

1hm2A02
type: Pdb
references: 1hm2
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1hm2
cofactor binding residue: GLU 405;ASP 407;ASP 416;TYR 417(Calcium binding)

1hm3A02
type: Pdb
references: 1hm3
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1hm3
cofactor binding residue: GLU 405;ASP 407;ASP 416;TYR 417(Calcium binding)

1hmuA02
type: Pdb
references: 1hmu
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1hmu
cofactor binding residue: GLU 405;ASP 407;ASP 416;TYR 417(Calcium binding)

1hmwA02
type: Pdb
references: 1hmw
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1hmw
cofactor binding residue: GLU 405;ASP 407;ASP 416;TYR 417(Calcium binding)

Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
medline id: 99264394
references: http://www.ncbi.nlm.nih.gov/pubmed/10329169
journal: J Mol Biol
volume: 288
pages: 635-47
published year: 1999
authors:
  item: Fethiere J
  item: Eggimann B
  item: Cygler M
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: Q59288

Active site of chondroitin AC lyase revealed by the structure of enzyme-oligosaccharide complexes and mutagenesis.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
medline id: 21226222
references: http://www.ncbi.nlm.nih.gov/pubmed/11327856
journal: Biochemistry
volume: 40
pages: 2359-72
published year: 2001
authors:
  item: Huang W
  item: Boju L
  item: Tkalec L
  item: Su H
  item: Yang HO
  item: Gunay NS
  item: Linhardt RJ
  item: Kim YS
  item: Matte A
  item: Cygler M
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: Q59288

Development of an assay and determination of kinetic parameters for chondroitin AC lyase using defined synthetic substrates.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12234466
journal: Anal Biochem
volume: 308
pages: 77-82
published year: 2002
authors:
  item: Rye CS
  item: Withers SG

Role of arginine 292 in the catalytic activity of chondroitin AC lyase from Flavobacterium heparinum.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12044904
journal: Biochim Biophys Acta
volume: 1597
pages: 260-70
published year: 2002
authors:
  item: Capila I
  item: Wu Y
  item: Rethwisch DW
  item: Matte A
  item: Cygler M
  item: Linhardt RJ

Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12175234
journal: J Am Chem Soc
volume: 124
pages: 9756-67
published year: 2002
authors:
  item: Rye CS
  item: Withers SG

Synthesis and evaluation of potential inhibitors of chondroitin AC lyase from Flavobacterium heparinum.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12076149
journal: J Org Chem
volume: 67
pages: 4505-12
published year: 2002
authors:
  item: Rye CS
  item: Withers SG

Analogue
compound: Chondroitin 4-sulfate
pdb: 1hm3A01
ligand het code: GCU-NAG

Analogue
compound: Chondroitin 6-sulfate
pdb: 1hm3A01
ligand het code: GCU-NAG

ReferenceForCatalyticMechanism
reference index: 2
reference section: p.2369-2371, Fig.8
reference step: 2

ReferenceForCatalyticMechanism
reference index: 3
reference section: Fig.1