T00082
type: Enzyme
cath: Rossmann fold
cath: D-amino Acid Aminotransferase; Chain A, domain 1
cath: Dna Ligase; domain 1
ec: https://purl.uniprot.org/enzyme/4.1.1.21
uniprot: P09029
kegg enzyme: phosphoribosylaminoimidazole carboxylase
kegg enzyme: 5-phosphoribosyl-5-aminoimidazole carboxylase
kegg enzyme: 5-amino-1-ribosylimidazole 5-phosphate carboxylase
kegg enzyme: AIR carboxylase
kegg enzyme: 1-(5-phosphoribosyl)-5-amino-4-imidazolecarboxylate carboxy-lyase
kegg enzyme: ADE2
kegg enzyme: class II PurE
kegg enzyme: 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate carboxy-lyase
kegg pathway: Purine metabolism
kegg cofactor: Magnesium
kegg substrate: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
kegg substrate: CO2
kegg substrate: ATP
kegg substrate: H2O
kegg product: 1-(5-Phospho-D-ribosyl)-5-carboxyamino-imidazole
kegg product: ADP
kegg product: Orthophosphate
pdb: 1b6rA01
pdb: 1b6sA01
pdb: 1b6sB01
pdb: 1b6sC01
pdb: 1b6sD01
pdb: 1b6rA02
pdb: 1b6sA02
pdb: 1b6sB02
pdb: 1b6sC02
pdb: 1b6sD02
pdb: 1b6rA03
pdb: 1b6sA03
pdb: 1b6sB03
pdb: 1b6sC03
pdb: 1b6sD03
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6rA01
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sA01
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sB01
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sC01
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sD01
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6rA02
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sA02
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sB02
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sC02
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sD02
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6rA03
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sA03
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sB03
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sC03
pdb bound state: Unbound
  compound: 1-(5-Phospho-D-ribosyl)-5-aminoimidazole
  pdb: 1b6sD03
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6rA01
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sA01
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sB01
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sC01
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sD01
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6rA02
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sA02
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sB02
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sC02
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sD02
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6rA03
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sA03
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sB03
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sC03
pdb bound state: Unbound
  compound: CO2
  pdb: 1b6sD03
pdb bound state: Unbound
  compound: ATP
  pdb: 1b6rA01
pdb bound state: Unbound
  compound: ATP
  pdb: 1b6sA01
pdb bound state: Unbound
  compound: ATP
  pdb: 1b6sB01
pdb bound state: Unbound
  compound: ATP
  pdb: 1b6sC01
pdb bound state: Unbound
  compound: ATP
  pdb: 1b6sD01
pdb bound state: Unbound
  compound: ATP
  pdb: 1b6rA02
pdb bound state: Unbound
  compound: ATP
  pdb: 1b6sA02
active site residues resources: 1b6s
references: Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
references: Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms.
references: http://ezcatdb.cbrc.jp/EzCatDB/reference/T00082#3
references: Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.

Rossmann fold
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.40.50.20

D-amino Acid Aminotransferase; Chain A, domain 1
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.30.470.20
catalytic domain: index.html

Dna Ligase; domain 1
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.30.1490.20

Purine metabolism
type: KeggPathway
references: KEGG:MAP00230

Magnesium
type: KeggCofactor
references: KEGG:C00305
compound type: divalent metal (Ca2+, Mg2+)
references: CHEBI:18420
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

1-(5-Phospho-D-ribosyl)-5-aminoimidazole
type: KeggSubstrate
references: KEGG:C03373
compound type: amine group
compound type: nucleotide
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

CO2
type: KeggSubstrate
references: KEGG:C00011
compound type: others
references: CHEBI:16526
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

ATP
type: KeggSubstrate
references: KEGG:C00002
compound type: amine group
compound type: nucleotide
references: CHEBI:15422
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

H2O
type: KeggSubstrate
references: KEGG:C00001
compound type: H2O
references: CHEBI:15377
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

1-(5-Phospho-D-ribosyl)-5-carboxyamino-imidazole
type: KeggProduct
references: KEGG:L00008
compound type: amide group
compound type: carboxyl group
compound type: nucleotide
references: CHEBI:48000
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

ADP
type: KeggProduct
references: KEGG:C00008
compound type: amine group
compound type: nucleotide
references: CHEBI:16761
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

Orthophosphate
type: KeggProduct
references: KEGG:C00009
compound type: phosphate group/phosphate ion
references: CHEBI:26078
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

1b6rA02
type: Pdb
references: 1b6r
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1b6r
modified residue: GLU 226;GLU 238(magnesium binding)

1b6sA02
type: Pdb
references: 1b6s
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1b6s
modified residue: GLU 226;GLU 238(magnesium binding)

1b6sB02
type: Pdb
references: 1b6s
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1b6s
modified residue: GLU 226;GLU 238(magnesium binding)

1b6sC02
type: Pdb
references: 1b6s
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1b6s
modified residue: GLU 226;GLU 238(magnesium binding)

1b6sD02
type: Pdb
references: 1b6s
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1b6s
modified residue: GLU 226;GLU 238(magnesium binding)

Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
type: BibliographicResource
comment: CHARACTERIZATION, AND SEQUENCE OF 1-20.
medline id: 92287929
references: http://www.ncbi.nlm.nih.gov/pubmed/1534690
journal: Biochemistry
volume: 31
pages: 5022-32
published year: 1992
authors:
  item: Meyer E
  item: Leonard NJ
  item: Bhat B
  item: Stubbe J
  item: Smith JM
references: P09029

Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/7918411
journal: Biochemistry
volume: 33
pages: 11927-34
published year: 1994
authors:
  item: Firestine SM
  item: Poon SW
  item: Mueller EJ
  item: Stubbe J
  item: Davisson VJ

http://ezcatdb.cbrc.jp/EzCatDB/reference/T00082#3
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10074353
journal: Biochemistry
volume: 38
pages: 3012-8
published year: 1999
authors:
  item: Meyer E
  item: Kappock TJ
  item: Osuji C
  item: Stubbe J
title: Evidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase.

Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
medline id: 20039871
references: http://www.ncbi.nlm.nih.gov/pubmed/10569930
journal: Biochemistry
volume: 38
pages: 15480-92
published year: 1999
authors:
  item: Thoden JB
  item: Kappock TJ
  item: Stubbe J
  item: Holden HM
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P09029

Analogue
compound: Orthophosphate
pdb: 1b6rA02
ligand het code: SO4

ReferenceForCatalyticMechanism
reference index: 4
reference section: p.15489, p.15491