T00007
type: Enzyme
cath: Butyryl-Coa Dehydrogenase, subunit A; domain 1
cath: Butyryl-CoA Dehydrogenase, subunit A; domain 2
cath: Butyryl-CoA Dehydrogenase, subunit A; domain 3
ec: https://purl.uniprot.org/enzyme/1.3.8.1
uniprot: Q06319
uniprot: P15651
kegg enzyme: short-chain acyl-CoA dehydrogenase
kegg enzyme: butyryl-CoA dehydrogenase
kegg enzyme: butanoyl-CoA dehydrogenase
kegg enzyme: butyryl dehydrogenase
kegg enzyme: unsaturated acyl-CoA reductase
kegg enzyme: ethylene reductase
kegg enzyme: enoyl-coenzyme A reductase
kegg enzyme: unsaturated acyl coenzyme A reductase
kegg enzyme: butyryl coenzyme A dehydrogenase
kegg enzyme: short-chain acyl CoA dehydrogenase
kegg enzyme: short-chain acyl-coenzyme A dehydrogenase
kegg enzyme: 3-hydroxyacyl CoA reductase
kegg enzyme: butanoyl-CoA:(acceptor) 2,3-oxidoreductase
kegg enzyme: ACADS (gene name)
kegg pathway: Fatty acid metabolism
kegg pathway: Valine, leucine and isoleucine degradation
kegg pathway: Butanoate metabolism
kegg cofactor: FAD
kegg substrate: Butanoyl-CoA
kegg substrate: Electron-transferring flavoprotein
kegg product: 2-Butenoyl-CoA
kegg product: Reduced electron-transferring flavoprotein
pdb: 1bucA01
pdb: 1bucB01
pdb: 1jqiA01
pdb: 1jqiB01
pdb: 1bucA02
pdb: 1bucB02
pdb: 1jqiA02
pdb: 1jqiB02
pdb: 1bucA03
pdb: 1bucB03
pdb: 1jqiA03
pdb: 1jqiB03
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1bucA01
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1bucB01
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1jqiA01
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1jqiB01
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1bucA02
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1bucB02
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1jqiA02
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1jqiB02
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1bucA03
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1bucB03
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1jqiA03
pdb bound state: Unbound
  compound: Butanoyl-CoA
  pdb: 1jqiB03
pdb bound state: Unbound
  compound: Electron-transferring flavoprotein
  pdb: 1bucA01
pdb bound state: Unbound
  compound: Electron-transferring flavoprotein
  pdb: 1bucB01
pdb bound state: Unbound
  compound: Electron-transferring flavoprotein
  pdb: 1jqiA01
pdb bound state: Unbound
  compound: Electron-transferring flavoprotein
  pdb: 1jqiB01
pdb bound state: Unbound
  compound: Electron-transferring flavoprotein
  pdb: 1bucA02
pdb bound state: Unbound
  compound: Electron-transferring flavoprotein
  pdb: 1bucB02
pdb bound state: Unbound
  compound: Electron-transferring flavoprotein
  pdb: 1jqiA02
active site residues resources: Functional role of the active site glutamate-368 in rat short chain acyl-CoA dehydrogenase.
active site residues resources: Q06319
references: Kinetic methods for the study of the enzyme systems of beta-oxidation.
references: Butyryl-CoA dehydrogenase from Megasphaera elsdenii. Specificity of the catalytic reaction.
references: Mechanistic studies with general acyl-CoA dehydrogenase and butyryl-CoA dehydrogenase: evidence for the transfer of the beta-hydrogen to the flavin N(5)-position as a hydride.
references: Characterization of wild-type and an active-site mutant in Escherichia coli of short-chain acyl-CoA dehydrogenase from Megasphaera elsdenii.
references: Oxidation-reduction properties of short-chain acyl-CoA dehydrogenase: effects of substrate analogs.
references: Product binding modulates the thermodynamic properties of a Megasphaera elsdenii short-chain acyl-CoA dehydrogenase active-site mutant.
references: Three-dimensional structure of butyryl-CoA dehydrogenase from Megasphaera elsdenii.
references: Functional role of the active site glutamate-368 in rat short chain acyl-CoA dehydrogenase.
references: Redesigning the active-site of an acyl-CoA dehydrogenase: new evidence supporting a one-base mechanism.
references: Role of glutamate 144 and glutamate 164 in the catalytic mechanism of enoyl-CoA hydratase.
references: Role of aromatic stacking interactions in the modulation of the two-electron reduction potentials of flavin and substrate/product in Megasphaera elsdenii short-chain acyl-coenzyme A dehydrogenase.
references: Purification and characterization of two polymorphic variants of short chain acyl-CoA dehydrogenase reveal reduction of catalytic activity and stability of the Gly185Ser enzyme.
references: Crystal structure of rat short chain acyl-CoA dehydrogenase complexed with acetoacetyl-CoA: comparison with other acyl-CoA dehydrogenases.
references: Comparison of ligand polarization and enzyme activation in medium- and short-chain acyl-coenzyme A dehydrogenase-novel analog complexes.
references: Misfolding, degradation, and aggregation of variant proteins. The molecular pathogenesis of short chain acyl-CoA dehydrogenase (SCAD) deficiency.
references: Structures of isobutyryl-CoA dehydrogenase and enzyme-product complex: comparison with isovaleryl- and short-chain acyl-CoA dehydrogenases.
references: Mammalian electron transferring flavoprotein.flavoprotein dehydrogenase complexes observed by microelectrospray ionization-mass spectrometry and surface plasmon resonance.
comment: This enzyme is a member of short-chain acyl-CoA dehydrogenases. FAD is bound to PHE 126;LEU 128;THR 129;THR 135;THR 162;GLN 283;THR 369;GLU 371 in the PDB entry (1buc).

Butyryl-Coa Dehydrogenase, subunit A; domain 1
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/1.10.540.10

Butyryl-CoA Dehydrogenase, subunit A; domain 2
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/2.40.110.10

Butyryl-CoA Dehydrogenase, subunit A; domain 3
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/1.20.140.10
catalytic domain: index.html

Fatty acid metabolism
type: KeggPathway
references: KEGG:MAP00071

Valine, leucine and isoleucine degradation
type: KeggPathway
references: KEGG:MAP00280

Butanoate metabolism
type: KeggPathway
references: KEGG:MAP00650

FAD
type: KeggCofactor
references: KEGG:C00016
compound type: amide group
compound type: amine group
compound type: aromatic ring (only carbon atom)
compound type: aromatic ring (with nitrogen atoms)
compound type: carbohydrate
compound type: nucleotide
references: CHEBI:16238
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

Butanoyl-CoA
type: KeggSubstrate
references: KEGG:C00136
compound type: amine group
compound type: carbohydrate
compound type: nucleotide
compound type: peptide/protein
compound type: sulfide group
references: CHEBI:15517
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

Electron-transferring flavoprotein
type: KeggSubstrate
references: KEGG:C04253
compound type: amide group
compound type: amine group
compound type: aromatic ring (only carbon atom)
compound type: aromatic ring (with nitrogen atoms)
compound type: carbohydrate
compound type: peptide/protein
compound type: phosphate group/phosphate ion

2-Butenoyl-CoA
type: KeggProduct
references: KEGG:C00877
compound type: amine group
compound type: carbohydrate
compound type: nucleotide
compound type: peptide/protein
compound type: sulfide group
references: CHEBI:15473
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

Reduced electron-transferring flavoprotein
type: KeggProduct
references: KEGG:C04570
compound type: amide group
compound type: amine group
compound type: aromatic ring (only carbon atom)
compound type: aromatic ring (with nitrogen atoms)
compound type: carbohydrate
compound type: peptide/protein
compound type: phosphate group/phosphate ion

1bucA03
type: Pdb
references: 1buc
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1buc
catalytic residue: GLU 367

1bucB03
type: Pdb
references: 1buc
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1buc
catalytic residue: GLU 367

1jqiA03
type: Pdb
references: 1jqi
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1jqi
catalytic residue: GLU 368

1jqiB03
type: Pdb
references: 1jqi
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1jqi
catalytic residue: GLU 768

Kinetic methods for the study of the enzyme systems of beta-oxidation.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/6639077
journal: Arch Biochem Biophys
volume: 227
pages: 21-30
published year: 1983
authors:
  item: Reinsch J
  item: Rojas C
  item: McFarland JT

Butyryl-CoA dehydrogenase from Megasphaera elsdenii. Specificity of the catalytic reaction.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/6712628
journal: Biochem J
volume: 218
pages: 521-9
published year: 1984
authors:
  item: Williamson G
  item: Engel PC

Mechanistic studies with general acyl-CoA dehydrogenase and butyryl-CoA dehydrogenase: evidence for the transfer of the beta-hydrogen to the flavin N(5)-position as a hydride.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/6466635
journal: Biochemistry
volume: 23
pages: 3154-61
published year: 1984
authors:
  item: Ghisla S
  item: Thorpe C
  item: Massey V

Characterization of wild-type and an active-site mutant in Escherichia coli of short-chain acyl-CoA dehydrogenase from Megasphaera elsdenii.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8399220
journal: Biochemistry
volume: 32
pages: 10736-42
published year: 1993
authors:
  item: Becker DF
  item: Fuchs JA
  item: Banfield DK
  item: Funk WD
  item: MacGillivray RT
  item: Stankovich MT

Oxidation-reduction properties of short-chain acyl-CoA dehydrogenase: effects of substrate analogs.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8080271
journal: Arch Biochem Biophys
volume: 313
pages: 261-6
published year: 1994
authors:
  item: Pace CP
  item: Stankovich MT

Product binding modulates the thermodynamic properties of a Megasphaera elsdenii short-chain acyl-CoA dehydrogenase active-site mutant.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8003473
journal: Biochemistry
volume: 33
pages: 7082-7
published year: 1994
authors:
  item: Becker DF
  item: Fuchs JA
  item: Stankovich MT

Three-dimensional structure of butyryl-CoA dehydrogenase from Megasphaera elsdenii.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
medline id: 95161388
references: http://www.ncbi.nlm.nih.gov/pubmed/7857927
journal: Biochemistry
volume: 34
pages: 2163-71
published year: 1995
authors:
  item: Djordjevic S
  item: Pace CP
  item: Stankovich MT
  item: Kim JJ
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: Q06319

Functional role of the active site glutamate-368 in rat short chain acyl-CoA dehydrogenase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8952487
journal: Biochemistry
volume: 35
pages: 15356-63
published year: 1996
authors:
  item: Battaile KP
  item: Mohsen AW
  item: Vockley J

Redesigning the active-site of an acyl-CoA dehydrogenase: new evidence supporting a one-base mechanism.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9459013
journal: Bioorg Med Chem
volume: 5
pages: 2157-64
published year: 1997
authors:
  item: Dakoji S
  item: Shin I
  item: Battaile KP
  item: Vockley J
  item: Liu HW

Role of glutamate 144 and glutamate 164 in the catalytic mechanism of enoyl-CoA hydratase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10413528
journal: Biochemistry
volume: 38
pages: 9508-16
published year: 1999
authors:
  item: Hofstein HA
  item: Feng Y
  item: Anderson VE
  item: Tonge PJ

Role of aromatic stacking interactions in the modulation of the two-electron reduction potentials of flavin and substrate/product in Megasphaera elsdenii short-chain acyl-coenzyme A dehydrogenase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/11412126
journal: Biochemistry
volume: 40
pages: 7720-8
published year: 2001
authors:
  item: Pellett JD
  item: Becker DF
  item: Saenger AK
  item: Fuchs JA
  item: Stankovich MT

Purification and characterization of two polymorphic variants of short chain acyl-CoA dehydrogenase reveal reduction of catalytic activity and stability of the Gly185Ser enzyme.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12220177
journal: Biochemistry
volume: 41
pages: 11126-33
published year: 2002
authors:
  item: Nguyen TV
  item: Riggs C
  item: Babovic-Vuksanovic D
  item: Kim YS
  item: Carpenter JF
  item: Burghardt TP
  item: Gregersen N
  item: Vockley J

Crystal structure of rat short chain acyl-CoA dehydrogenase complexed with acetoacetyl-CoA: comparison with other acyl-CoA dehydrogenases.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/11812788
journal: J Biol Chem
volume: 277
pages: 12200-7
published year: 2002
authors:
  item: Battaile KP
  item: Molin-Case J
  item: Paschke R
  item: Wang M
  item: Bennett D
  item: Vockley J
  item: Kim JJ

Comparison of ligand polarization and enzyme activation in medium- and short-chain acyl-coenzyme A dehydrogenase-novel analog complexes.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12504892
journal: Arch Biochem Biophys
volume: 409
pages: 251-61
published year: 2003
authors:
  item: Lamm TR
  item: Kohls TD
  item: Saenger AK
  item: Stankovich MT

Misfolding, degradation, and aggregation of variant proteins. The molecular pathogenesis of short chain acyl-CoA dehydrogenase (SCAD) deficiency.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/14506246
journal: J Biol Chem
volume: 278
pages: 47449-58
published year: 2003
authors:
  item: Pedersen CB
  item: Bross P
  item: Winter VS
  item: Corydon TJ
  item: Bolund L
  item: Bartlett K
  item: Vockley J
  item: Gregersen N

Structures of isobutyryl-CoA dehydrogenase and enzyme-product complex: comparison with isovaleryl- and short-chain acyl-CoA dehydrogenases.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/14752098
journal: J Biol Chem
volume: 279
pages: 16526-34
published year: 2004
authors:
  item: Battaile KP
  item: Nguyen TV
  item: Vockley J
  item: Kim JJ

Mammalian electron transferring flavoprotein.flavoprotein dehydrogenase complexes observed by microelectrospray ionization-mass spectrometry and surface plasmon resonance.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/14744856
journal: J Biol Chem
volume: 279
pages: 13786-91
published year: 2004
authors:
  item: Hoard-Fruchey HM
  item: Goetzman E
  item: Benson L
  item: Naylor S
  item: Vockley J

Analogue
compound: 2-Butenoyl-CoA
pdb: 1bucA03
ligand het code: CAA

Analogue
compound: 2-Butenoyl-CoA
pdb: 1bucB03
ligand het code: CAA

Analogue
compound: 2-Butenoyl-CoA
pdb: 1jqiA03
ligand het code: CAA

Analogue
compound: 2-Butenoyl-CoA
pdb: 1jqiB03
ligand het code: CAA

ReferenceForCatalyticMechanism
reference index: 3
reference section: Scheme III, p.3160

ReferenceForCatalyticMechanism
reference index: 6
reference section: Fig.5

ReferenceForCatalyticMechanism
reference index: 7
reference section: p.2166-2167, p.2170-2171

ReferenceForCatalyticMechanism
reference index: 8
reference section: p.15361

ReferenceForCatalyticMechanism
reference index: 9
reference section: Scheme 5A, p.2161

ReferenceForCatalyticMechanism
reference index: 11