D00538
type: Enzyme
rlcp: p974
cath: Jelly Rolls
ec: https://purl.uniprot.org/enzyme/3.2.1.8
uniprot: P77853
kegg enzyme: endo-1,4-beta-xylanase
kegg enzyme: endo-(1->4)-beta-xylan 4-xylanohydrolase
kegg enzyme: endo-1,4-xylanase
kegg enzyme: xylanase
kegg enzyme: beta-1,4-xylanase
kegg enzyme: endo-beta-1,4-xylanase
kegg enzyme: endo-1,4-beta-D-xylanase
kegg enzyme: 1,4-beta-xylan xylanohydrolase
kegg enzyme: beta-xylanase
kegg enzyme: beta-1,4-xylan xylanohydrolase
kegg enzyme: beta-D-xylanase
kegg substrate: Xylan
kegg substrate: H2O
kegg product: Xylan
pdb: 1f5jA
pdb: 1f5jB
pdb bound state: Unbound
  compound: Xylan
  pdb: 1f5jA
pdb bound state: Unbound
  compound: Xylan
  pdb: 1f5jB
pdb bound state: Unbound
  compound: Xylan
  pdb: 1f5jA
pdb bound state: Unbound
  compound: Xylan
  pdb: 1f5jB
pdb bound state: Unbound
  pdb: 1f5jA
pdb bound state: Unbound
  pdb: 1f5jB
reference for catalytic mechanism: ReferenceForCatalyticMechanism
  reference index: 2
  reference section: p.68
reference for catalytic mechanism: ReferenceForCatalyticMechanism
  reference index: 3
  reference section: p.2499-2500
reference for catalytic mechanism: ReferenceForCatalyticMechanism
  reference index: 7
  reference section: Fig.1, p.471-473
reference for catalytic mechanism: ReferenceForCatalyticMechanism
  reference index: 8
  reference section: p.851-853
reference for catalytic mechanism: ReferenceForCatalyticMechanism
  reference index: 10
  reference section: p.9624
references: Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
references: High-resolution structures of xylanases from B.circulans and T.harzianum identify a new folding pattern and implications for the atomic basis of the catalysis.
references: Three-dimensional structure of endo-1,4-beta-xylanase II from Trichoderma reesei: two conformational states in the active site.
references: Stereochemistry of the hydrolysis of glycosidic linkage by endo-beta-1,4-xylanases of Trichoderma reesei.
references: Crystallization and preliminary X-ray crystallographic studies of thermostable xylanase crystals isolated from Paecilomyces varioti.
references: Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds.
references: Mutational and crystallographic analyses of the active site residues of the Bacillus circulans xylanase.
references: Structural comparison of two major endo-1,4-xylanases from Trichoderma reesei.
references: Characterisation of 4-deoxy-beta-L-threo-hex-4-enopyranosyluronic acid attached to xylan in pine kraft pulp and pulping liquor by 1H and 13C NMR spectroscopy.
references: Covalent binding of three epoxyalkyl xylosides to the active site of endo-1,4-xylanase II from Trichoderma reesei.
references: Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity.
references: The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of bacillus circulans xylanase.
references: Three-dimensional structure of Endo-1,4-beta-xylanase I from Aspergillus niger: molecular basis for its low pH optimum.
references: Xylanase homology modeling using the inverse protein folding approach.
references: Characterization of a buried neutral histidine residue in Bacillus circulans xylanase: NMR assignments, pH titration, and hydrogen exchange.
references: Secondary structure and NMR assignments of Bacillus circulans xylanase.
references: Endo-beta-1,4-xylanase families: differences in catalytic properties.
references: Structural and functional properties of low molecular weight endo-1,4-beta-xylanases.
references: Complete measurement of the pKa values of the carboxyl and imidazole groups in Bacillus circulans xylanase.
references: Catalytic thiol and carboxylate: role of cysteine and glutamic acid in the xylosidic activity of endoxylanase from Chainia sp. (NCL 82-5-1).
references: Mechanisms of cellulases and xylanases.
references: Characterization of a buried neutral histidine in Bacillus circulans xylanase: internal dynamics and interaction with a bound water molecule.
references: Thermophilic xylanase from Thermomyces lanuginosus: high-resolution X-ray structure and modeling studies.
references: Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH.
references: Functional conformational changes of endo-1,4-xylanase II from Trichoderma reesei: a molecular dynamics study.
references: Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase.
references: Purification and biochemical characteristics of beta-D-xylanase from a thermophilic fungus, Thermomyces lanuginosus-SSBP.
references: Catalysis and specificity in enzymatic glycoside hydrolysis: a 2,5B conformation for the glycosyl-enzyme intermediate revealed by the structure of the Bacillus agaradhaerens family 11 xylanase.
references: Family 10 and 11 xylanase genes from Caldicellulosiruptor sp. strain Rt69B.1.
references: Structure of XynB, a highly thermostable beta-1,4-xylanase from Dictyoglomus thermophilum Rt46B.1, at 1.8 A resolution.
references: Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei.
references: Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase.
references: The tertiary structure at 1.59 A resolution and the proposed amino acid sequence of a family-11 xylanase from the thermophilic fungus Paecilomyces varioti bainier.
references: Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate.
references: Oligosaccharide binding to family 11 xylanases: both covalent intermediate and mutant product complexes display (2,5)B conformations at the active centre.
references: Crystallographic analysis of family 11 endo-beta-1,4-xylanase Xyl1 from Streptomyces sp. S38.
references: Dissecting the electrostatic interactions and pH-dependent activity of a family 11 glycosidase.
references: Simultaneous catalysis and product separation by cross-linked enzyme crystals.
references: ACEII, a novel transcriptional activator involved in regulation of cellulase and xylanase genes of Trichoderma reesei.
references: A combination of weakly stabilizing mutations with a disulfide bridge in the alpha-helix region of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermal stability through synergism.
references: Specific characterization of substrate and inhibitor binding sites of a glycosyl hydrolase family 11 xylanase from Aspergillus niger.
references: Engineering of multiple arginines into the Ser/Thr surface of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermotolerance and shifts the pH optimum towards alkaline pH.
references: Mapping the conformational itinerary of beta-glycosidases by X-ray crystallography.
references: MM-PBSA free energy analysis of endo-1,4-xylanase II (XynII)-substrate complexes: binding of the reactive sugar in a skew boat and chair conformation.
references: Long-range nature of the interactions between titratable groups in Bacillus agaradhaerens family 11 xylanase: pH titration of B. agaradhaerens xylanase.
references: Structural basis for inhibition of Aspergillus niger xylanase by triticum aestivum xylanase inhibitor-I.
comment: There are many kinds of xylanases, family-2b, family-10, & family-11. This enzyme belongs to the glycosidase family-11 along with the homologous enzyme (S00151 in EzCatDB), while the entries D00169, D00479 & M00160 belong to family-10. According to the literature [7], [18], [26] & [28], the catalytic reaction proceeds as follows: (1) Glu180 acts as a general acid to protonate the leaving oxygen atom, leading to a oxocarbenium-like transtion state from xylan substrate. (2) pKa of Glu90, which acts as a nucleophile, is modulated by Tyr81 and Tyr92. Glu90 makes a nucleophilic attack on C1 atom of the transition state, forming a glycosyl-enzyme intermediate. (2') As the glycosyl-enzyme intermediate is formed, Tyr81 switched from Glu90 to Glu180. Tyr92 may modulate the activity of Glu180 as a general base. (3) Glu170 acts as a general base to activate a water molecule. (4) The activated water makes a nucleophilic attack on the C1 atom of the intermediate. This reaction proceeds through an oxocarbenium-like transition state to complete the hydrolysis. ### According to the literature [26] & [28], the homologous enzyme of this enzyme (S00151 in EzCatDB) adopts 2,5B (or Boat) conformation, which places C5, O5, C1 and C2 in a planar arrangement, for the intermediate. Moreover, at the position of 37 of 1bk1 (see S00151), the family-11 has an aspartic acid or an aspargine residue. For high pH optimum, aspartic acid residue is used at the position, whilst an aspargine residue is adopted for lower pH optimum, according to the literature [13]. This residue makes a hydrogen bond with the acid-base, Glu170, modulating its pKa.

Jelly Rolls
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/2.60.120.180
catalytic domain: index.html

Jelly Rolls
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/2.60.120.-

Xylan
type: KeggSubstrate
references: KEGG:C00707
compound type: polysaccharide
type: KeggProduct

H2O
type: KeggSubstrate
references: KEGG:C00001
compound type: H2O
references: CHEBI:15377
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

1f5jA
type: Pdb
references: 1f5j
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1f5j
catalytic residue: TYR 81;GLU 90;TYR 92;GLU 180

1f5jB
type: Pdb
references: 1f5j
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1f5j
catalytic residue: TYR 81;GLU 90;TYR 92;GLU 180

Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/1886523
journal: Microbiol Rev
volume: 55
pages: 303-15
published year: 1991
authors:
  item: Gilkes NR
  item: Henrissat B
  item: Kilburn DG
  item: Miller RC Jr
  item: Warren RA

High-resolution structures of xylanases from B.circulans and T.harzianum identify a new folding pattern and implications for the atomic basis of the catalysis.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) (ISBN: 9518913625)
journal: TRICEL Symposium on Trichoderma Reesei Cellulases and Other Hydrolases
pages: 63-72
published year: 1993
authors:
  item: Campbell RL
  item: Rose DR
  item: Wakarchuk WW
  item: To RJ
  item: Sung W
  item: Yaguchi M
references: P48793

Three-dimensional structure of endo-1,4-beta-xylanase II from Trichoderma reesei: two conformational states in the active site.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
medline id: 94283373
references: http://www.ncbi.nlm.nih.gov/pubmed/8013449
journal: EMBO J
volume: 13
pages: 2493-501
published year: 1994
authors:
  item: Torronen A
  item: Harkki A
  item: Rouvinen J
references: P36217

Stereochemistry of the hydrolysis of glycosidic linkage by endo-beta-1,4-xylanases of Trichoderma reesei.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/7988708
journal: FEBS Lett
volume: 356
pages: 137-40
published year: 1994
authors:
  item: Biely P
  item: Kremnicky L
  item: Alfoldi J
  item: Tenkanen M

Crystallization and preliminary X-ray crystallographic studies of thermostable xylanase crystals isolated from Paecilomyces varioti.
type: BibliographicResource
comment: X-ray crystallography
references: http://www.ncbi.nlm.nih.gov/pubmed/7966300
journal: J Mol Biol
volume: 243
pages: 806-8
published year: 1994
authors:
  item: Eswaramoorthy S
  item: Vithayathil PJ
  item: Viswamitra MA
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/7700870
journal: Protein Eng
volume: 7
pages: 1379-86
published year: 1994
authors:
  item: Wakarchuk WW
  item: Sung WL
  item: Campbell RL
  item: Cunningham A
  item: Watson DC
  item: Yaguchi M

Mutational and crystallographic analyses of the active site residues of the Bacillus circulans xylanase.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), AND MUTAGENESIS.
medline id: 94290322
references: http://www.ncbi.nlm.nih.gov/pubmed/8019418
journal: Protein Sci
volume: 3
pages: 467-75
published year: 1994
authors:
  item: Wakarchuk WW
  item: Campbell RL
  item: Sung WL
  item: Davoodi J
  item: Yaguchi M
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P09850

Structural comparison of two major endo-1,4-xylanases from Trichoderma reesei.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
medline id: 95127663
references: http://www.ncbi.nlm.nih.gov/pubmed/7827044
journal: Biochemistry
volume: 34
pages: 847-56
published year: 1995
authors:
  item: Torronen A
  item: Rouvinen J
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P36218
references: P36217

Characterisation of 4-deoxy-beta-L-threo-hex-4-enopyranosyluronic acid attached to xylan in pine kraft pulp and pulping liquor by 1H and 13C NMR spectroscopy.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/7648585
journal: Carbohydr Res
volume: 272
pages: 55-71
published year: 1995
authors:
  item: Teleman A
  item: Harjunpaa V
  item: Tenkanen M
  item: Buchert J
  item: Hausalo T
  item: Drakenberg T
  item: Vuorinen T

Covalent binding of three epoxyalkyl xylosides to the active site of endo-1,4-xylanase II from Trichoderma reesei.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
medline id: 96302263
references: http://www.ncbi.nlm.nih.gov/pubmed/8755744
journal: Biochemistry
volume: 35
pages: 9617-24
published year: 1996
authors:
  item: Havukainen R
  item: Torronen A
  item: Laitinen T
  item: Rouvinen J
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P36217

Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8756474
journal: Biochemistry
volume: 35
pages: 10110-8
published year: 1996
authors:
  item: Lawson SL
  item: Wakarchuk WW
  item: Withers SG

The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of bacillus circulans xylanase.
type: BibliographicResource
comment: STRUCTURE BY NMR.
medline id: 96322313
references: http://www.ncbi.nlm.nih.gov/pubmed/8756457
journal: Biochemistry
volume: 35
pages: 9958-66
published year: 1996
authors:
  item: McIntosh LP
  item: Hand G
  item: Johnson PE
  item: Joshi MD
  item: Korner M
  item: Plesniak LA
  item: Ziser L
  item: Wakarchuk WW
  item: Withers SG
references: P09850

Three-dimensional structure of Endo-1,4-beta-xylanase I from Aspergillus niger: molecular basis for its low pH optimum.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
medline id: 97045991
references: http://www.ncbi.nlm.nih.gov/pubmed/8890913
journal: J Mol Biol
volume: 263
pages: 70-8
published year: 1996
authors:
  item: Krengel U
  item: Dijkstra BW
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P55329

Xylanase homology modeling using the inverse protein folding approach.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8845760
journal: Protein Sci
volume: 5
pages: 705-8
published year: 1996
authors:
  item: Chen X
  item: Whitmire D
  item: Bowen JP

Characterization of a buried neutral histidine residue in Bacillus circulans xylanase: NMR assignments, pH titration, and hydrogen exchange.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8931150
journal: Protein Sci
volume: 5
pages: 2319-28
published year: 1996
authors:
  item: Plesniak LA
  item: Connelly GP
  item: Wakarchuk WW
  item: McIntosh LP

Secondary structure and NMR assignments of Bacillus circulans xylanase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8762143
journal: Protein Sci
volume: 5
pages: 1118-35
published year: 1996
authors:
  item: Plesniak LA
  item: Wakarchuk WW
  item: McIntosh LP

Endo-beta-1,4-xylanase families: differences in catalytic properties.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9335171
journal: J Biotechnol
volume: 57
pages: 151-66
published year: 1997
authors:
  item: Biely P
  item: Vrsanska M
  item: Tenkanen M
  item: Kluepfel D

Structural and functional properties of low molecular weight endo-1,4-beta-xylanases.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9335170
journal: J Biotechnol
volume: 57
pages: 137-49
published year: 1997
authors:
  item: Torronen A
  item: Rouvinen J

Complete measurement of the pKa values of the carboxyl and imidazole groups in Bacillus circulans xylanase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9416621
journal: Protein Sci
volume: 6
pages: 2667-70
published year: 1997
authors:
  item: Joshi MD
  item: Hedberg A
  item: McIntosh LP

Catalytic thiol and carboxylate: role of cysteine and glutamic acid in the xylosidic activity of endoxylanase from Chainia sp. (NCL 82-5-1).
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9675021
journal: Arch Biochem Biophys
volume: 355
pages: 153-9
published year: 1998
authors:
  item: Subray SH
  item: Ameeta RK
  item: Krishna NG
  item: Khan IM

Mechanisms of cellulases and xylanases.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9649739
journal: Biochem Soc Trans
volume: 26
pages: 156-60
published year: 1998
authors:
  item: Birsan C
  item: Johnson P
  item: Joshi M
  item: MacLeod A
  item: McIntosh L
  item: Monem V
  item: Nitz M
  item: Rose DR
  item: Tull D
  item: Wakarchuck WW
  item: Wang Q
  item: Warren RA
  item: White A
  item: Withers SG

Characterization of a buried neutral histidine in Bacillus circulans xylanase: internal dynamics and interaction with a bound water molecule.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9485306
journal: Biochemistry
volume: 37
pages: 1810-8
published year: 1998
authors:
  item: Connelly GP
  item: McIntosh LP

Thermophilic xylanase from Thermomyces lanuginosus: high-resolution X-ray structure and modeling studies.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS).
medline id: 98426042
references: http://www.ncbi.nlm.nih.gov/pubmed/9753433
journal: Biochemistry
volume: 37
pages: 13475-85
published year: 1998
authors:
  item: Gruber K
  item: Klintschar G
  item: Hayn M
  item: Schlacher A
  item: Steiner W
  item: Kratky C
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: O43097

Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 29-210.
medline id: 99127851
references: http://www.ncbi.nlm.nih.gov/pubmed/9930661
journal: Protein Eng
volume: 11
pages: 1121-8
published year: 1998
authors:
  item: Fushinobu S
  item: Ito K
  item: Konno M
  item: Wakagi T
  item: Matsuzawa H
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P33557

Functional conformational changes of endo-1,4-xylanase II from Trichoderma reesei: a molecular dynamics study.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9626702
journal: Proteins
volume: 31
pages: 434-44
published year: 1998
authors:
  item: Muilu J
  item: Torronen A
  item: Perakyla M
  item: Rouvinen J

Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase.
type: BibliographicResource
comment: X-ray crystallography
references: http://www.ncbi.nlm.nih.gov/pubmed/10220321
journal: Biochemistry
volume: 38
pages: 5346-54
published year: 1999
authors:
  item: Sidhu G
  item: Withers SG
  item: Nguyen NT
  item: McIntosh LP
  item: Ziser L
  item: Brayer GD
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

Purification and biochemical characteristics of beta-D-xylanase from a thermophilic fungus, Thermomyces lanuginosus-SSBP.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10467122
journal: Biotechnol Appl Biochem
volume: 30
pages: 73-9
published year: 1999
authors:
  item: Lin J
  item: Ndlovu LM
  item: Singh S
  item: Pillay B

Catalysis and specificity in enzymatic glycoside hydrolysis: a 2,5B conformation for the glycosyl-enzyme intermediate revealed by the structure of the Bacillus agaradhaerens family 11 xylanase.
type: BibliographicResource
comment: X-ray crystallography
references: http://www.ncbi.nlm.nih.gov/pubmed/10381409
journal: Chem Biol
volume: 6
pages: 483-92
published year: 1999
authors:
  item: Sabini E
  item: Sulzenbacher G
  item: Dauter M
  item: Dauter Z
  item: Jorgensen PL
  item: Schulein M
  item: Dupont C
  item: Davies GJ
  item: Wilson KS
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

Family 10 and 11 xylanase genes from Caldicellulosiruptor sp. strain Rt69B.1.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10356996
journal: Extremophiles
volume: 3
pages: 103-11
published year: 1999
authors:
  item: Morris DD
  item: Gibbs MD
  item: Ford M
  item: Thomas J
  item: Bergquist PL

Structure of XynB, a highly thermostable beta-1,4-xylanase from Dictyoglomus thermophilum Rt46B.1, at 1.8 A resolution.
type: BibliographicResource
comment: X-ray crystallography
references: http://www.ncbi.nlm.nih.gov/pubmed/11053833
journal: Acta Crystallogr D Biol Crystallogr
volume: 56
pages: 1367-75
published year: 2000
authors:
  item: McCarthy AA
  item: Morris DD
  item: Bergquist PL
  item: Baker EN
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10981683
journal: FEMS Microbiol Lett
volume: 190
pages: 13-9
published year: 2000
authors:
  item: Te'o VS
  item: Cziferszky AE
  item: Bergquist PL
  item: Nevalainen KM

Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase.
type: BibliographicResource
comment: X-ray crystallography
references: http://www.ncbi.nlm.nih.gov/pubmed/10860737
journal: J Mol Biol
volume: 299
pages: 255-79
published year: 2000
authors:
  item: Joshi MD
  item: Sidhu G
  item: Pot I
  item: Brayer GD
  item: Withers SG
  item: McIntosh LP
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

The tertiary structure at 1.59 A resolution and the proposed amino acid sequence of a family-11 xylanase from the thermophilic fungus Paecilomyces varioti bainier.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10623548
journal: J Mol Biol
volume: 295
pages: 581-93
published year: 2000
authors:
  item: Kumar PR
  item: Eswaramoorthy S
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ReferenceForCatalyticMechanism
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reference section: Scheme 1

ReferenceForCatalyticMechanism
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ReferenceForCatalyticMechanism
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ReferenceForCatalyticMechanism
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ReferenceForCatalyticMechanism
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ReferenceForCatalyticMechanism
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ReferenceForCatalyticMechanism
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ReferenceForCatalyticMechanism
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ReferenceForCatalyticMechanism
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ReferenceForCatalyticMechanism
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ReferenceForCatalyticMechanism
reference index: 43
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ReferenceForCatalyticMechanism
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reference section: Fig. 1, p.3539