D00476
type: Enzyme
cath: Rossmann fold
cath: Dihydrodipicolinate Reductase; domain 2
ec: https://purl.uniprot.org/enzyme/1.2.1.59
csa: 1cf2
uniprot: P10618
uniprot: P39460
kegg enzyme: glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+)(phosphorylating)
kegg enzyme: triosephosphate dehydrogenase (NAD(P))
kegg enzyme: glyceraldehyde-3-phosphate dehydrogenase (NAD(P)) (phosphorylating)
kegg pathway: Glycolysis / Gluconeogenesis
kegg pathway: Carbon fixation in photosynthetic organisms
kegg substrate: D-Glyceraldehyde 3-phosphate
kegg substrate: Orthophosphate
kegg substrate: NAD+
kegg substrate: NADP+
kegg product: 3-Phospho-D-glyceroyl phosphate
kegg product: NADH
kegg product: NADPH
kegg product: H+
pdb: 1b7gO01
pdb: 1b7gQ01
pdb: 1cf2O01
pdb: 1cf2P01
pdb: 1cf2Q01
pdb: 1cf2R01
pdb: 1b7gO02
pdb: 1b7gQ02
pdb: 1cf2O02
pdb: 1cf2P02
pdb: 1cf2Q02
pdb: 1cf2R02
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1b7gO01
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1b7gQ01
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1cf2O01
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1cf2P01
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1cf2Q01
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1cf2R01
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1b7gO02
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1b7gQ02
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1cf2O02
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1cf2P02
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1cf2Q02
pdb bound state: Unbound
  compound: D-Glyceraldehyde 3-phosphate
  pdb: 1cf2R02
pdb bound state: Unbound
  compound: Orthophosphate
  pdb: 1b7gO01
pdb bound state: Unbound
  compound: Orthophosphate
  pdb: 1b7gQ01
pdb bound state: Unbound
  compound: Orthophosphate
  pdb: 1cf2O01
pdb bound state: Unbound
  compound: Orthophosphate
  pdb: 1cf2P01
pdb bound state: Unbound
  compound: Orthophosphate
  pdb: 1cf2Q01
pdb bound state: Unbound
  compound: Orthophosphate
  pdb: 1cf2R01
pdb bound state: Analogue
  compound: Orthophosphate
  pdb: 1b7gO02
  ligand het code: SO4_601
pdb bound state: Analogue
  compound: Orthophosphate
  pdb: 1b7gQ02
  ligand het code: SO4_602
pdb bound state: Analogue
  compound: Orthophosphate
  pdb: 1cf2O02
  ligand het code: SO4
pdb bound state: Analogue
  compound: Orthophosphate
  pdb: 1cf2P02
  ligand het code: SO4
pdb bound state: Analogue
  compound: Orthophosphate
  pdb: 1cf2Q02
  ligand het code: SO4
pdb bound state: Analogue
  compound: Orthophosphate
  pdb: 1cf2R02
  ligand het code: SO4
pdb bound state: Unbound
  compound: NAD+
  pdb: 1b7gO01
pdb bound state: Unbound
  compound: NAD+
  pdb: 1b7gQ01
pdb bound state: Unbound
  compound: NAD+
  pdb: 1cf2O01
pdb bound state: Unbound
  compound: NAD+
  pdb: 1cf2P01
pdb bound state: Unbound
  compound: NAD+
  pdb: 1cf2Q01
pdb bound state: Unbound
  compound: NAD+
  pdb: 1cf2R01
active site residues resources: The crystal structure of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus in the presence of NADP(+) at 2.1 A resolution.
active site residues resources: Protein thiol modification of glyceraldehyde-3-phosphate dehydrogenase and caspase-3 by nitric oxide.
active site residues resources: Comparative analysis of thermoadaptation within the archaeal glyceraldehyde-3-phosphate dehydrogenases from mesophilic Methanobacterium bryantii and thermophilic Methanothermus fervidus.
active site residues resources: 1b7g
references: Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.
references: D-Glyceraldehyde-3-phosphate dehydrogenase: structural basis and functional role of the acyl transfer reactions.
references: Crystallization and preliminary X-ray diffraction studies of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus.
references: http://ezcatdb.cbrc.jp/EzCatDB/reference/D00476#4
references: Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.
references: The crystal structure of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus in the presence of NADP(+) at 2.1 A resolution.
references: Protein thiol modification of glyceraldehyde-3-phosphate dehydrogenase and caspase-3 by nitric oxide.
references: Chloroplast glyceraldehyde-3-phosphate dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit.
references: Glyceraldehyde-3-phosphate dehydrogenase from Sulfolobus solfataricus.
references: Comparative analysis of thermoadaptation within the archaeal glyceraldehyde-3-phosphate dehydrogenases from mesophilic Methanobacterium bryantii and thermophilic Methanothermus fervidus.
references: Identification of tissue transglutaminase-reactive lysine residues in glyceraldehyde-3-phosphate dehydrogenase.
references: Hyperthermophilic dehydrogenase enzymes.
references: Brownian dynamics of interactions between glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mutants and F-actin.
comment: There are several types of glyceraldehyde-3-phosphate dehydrogenases: NADP-dependent GAPDH (E.C. 1.2.1.9) NAD(P)-dependent GAPDH (phosphorylating) (E.C. 1.2.1.59) GAPDH (phosphorylating) (E.C. 1.2.1.12) NADP-dependent GAPDH (phosphorylating) (E.C. 1.2.1.13) This enzyme corresponds to NAD(P)-dependent GAPDH (phosphorylating) (E.C. 1.2.1.59).

Rossmann fold
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.40.50.720

Dihydrodipicolinate Reductase; domain 2
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.30.360.10
catalytic domain: index.html

Glycolysis / Gluconeogenesis
type: KeggPathway
references: KEGG:MAP00010

Carbon fixation in photosynthetic organisms
type: KeggPathway
references: KEGG:MAP00710

D-Glyceraldehyde 3-phosphate
type: KeggSubstrate
references: KEGG:C00118
compound type: carbohydrate
compound type: phosphate group/phosphate ion
references: CHEBI:29052
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

Orthophosphate
type: KeggSubstrate
references: KEGG:C00009
compound type: phosphate group/phosphate ion
references: CHEBI:26078
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

NAD+
type: KeggSubstrate
references: KEGG:C00003
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:15846
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

NADP+
type: KeggSubstrate
references: KEGG:C00006
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:18009
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

3-Phospho-D-glyceroyl phosphate
type: KeggProduct
references: KEGG:C00236
compound type: carbohydrate
compound type: phosphate group/phosphate ion
references: CHEBI:16001
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

NADH
type: KeggProduct
references: KEGG:C00004
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:16908
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

NADPH
type: KeggProduct
references: KEGG:C00005
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:16474
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

H+
type: KeggProduct
references: KEGG:C00080
compound type: others
references: CHEBI:15378
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

1b7gO01
type: Pdb
references: 1b7g
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1b7g
comment: mutant I2V

1b7gQ01
type: Pdb
references: 1b7g
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1b7g
comment: mutant I2V

1b7gO02
type: Pdb
references: 1b7g
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1b7g
catalytic residue: CYS 139;HIS 219

1b7gQ02
type: Pdb
references: 1b7g
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1b7g
catalytic residue: CYS 139;HIS 219

1cf2O02
type: Pdb
references: 1cf2
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1cf2
catalytic residue: CYS 140;HIS 219

1cf2P02
type: Pdb
references: 1cf2
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1cf2
catalytic residue: CYS 140;HIS 219

1cf2Q02
type: Pdb
references: 1cf2
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1cf2
catalytic residue: CYS 140;HIS 219

1cf2R02
type: Pdb
references: 1cf2
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1cf2
catalytic residue: CYS 140;HIS 219

Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9761871
journal: Acta Crystallogr D Biol Crystallogr
volume: 54
pages: 671-4
published year: 1998
authors:
  item: Fleming TM
  item: Jones CE
  item: Piper PW
  item: Cowan DA
  item: Isupov MN
  item: Littlechild JA

D-Glyceraldehyde-3-phosphate dehydrogenase: structural basis and functional role of the acyl transfer reactions.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/9632884
journal: Biochemistry (Mosc)
volume: 63
pages: 504-15
published year: 1998
authors:
  item: Nagradova NK
  item: Schmalhausen EV

Crystallization and preliminary X-ray diffraction studies of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus.
type: BibliographicResource
comment: X-ray crystallography
references: http://www.ncbi.nlm.nih.gov/pubmed/10393306
journal: Acta Crystallogr D Biol Crystallogr
volume: 55
pages: 1353-5
published year: 1999
authors:
  item: Charron C
  item: Talfournier F
  item: Isupov MN
  item: Branlant G
  item: Littlechild JA
  item: Vitoux B
  item: Aubry A
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

http://ezcatdb.cbrc.jp/EzCatDB/reference/D00476#4
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/10491162
journal: Eur J Biochem
volume: 265
pages: 93-104
published year: 1999
authors:
  item: Talfournier F
  item: Colloc'h N
  item: Mornon JP
  item: Branlant G
title: Functional characterization of the phosphorylating D-glyceraldehyde 3-phosphate dehydrogenase from the archaeon Methanothermus fervidus by comparative molecular modelling and site-directed mutagenesis.

Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS)
medline id: 99380409
references: http://www.ncbi.nlm.nih.gov/pubmed/10448043
journal: J Mol Biol
volume: 291
pages: 651-60
published year: 1999
authors:
  item: Isupov MN
  item: Fleming TM
  item: Dalby AR
  item: Crowhurst GS
  item: Bourne PC
  item: Littlechild JA
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P39460

The crystal structure of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus in the presence of NADP(+) at 2.1 A resolution.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)
medline id: 20181873
references: http://www.ncbi.nlm.nih.gov/pubmed/10715215
journal: J Mol Biol
volume: 297
pages: 481-500
published year: 2000
authors:
  item: Charron C
  item: Talfournier F
  item: Isupov MN
  item: Littlechild JA
  item: Branlant G
  item: Vitoux B
  item: Aubry A
references: P10618

Protein thiol modification of glyceraldehyde-3-phosphate dehydrogenase and caspase-3 by nitric oxide.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12369901
journal: Curr Protein Pept Sci
volume: 2
pages: 61-72
published year: 2001
authors:
  item: Brune B
  item: Mohr S

Chloroplast glyceraldehyde-3-phosphate dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/11438534
journal: J Biol Chem
volume: 276
pages: 35247-52
published year: 2001
authors:
  item: Qi J
  item: Isupov MN
  item: Littlechild JA
  item: Anderson LE

Glyceraldehyde-3-phosphate dehydrogenase from Sulfolobus solfataricus.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/11265453
journal: Methods Enzymol
volume: 331
pages: 105-17
published year: 2001
authors:
  item: Littlechild JA
  item: Isupov M

Comparative analysis of thermoadaptation within the archaeal glyceraldehyde-3-phosphate dehydrogenases from mesophilic Methanobacterium bryantii and thermophilic Methanothermus fervidus.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/12382287
journal: Biopolymers
volume: 65
pages: 263-73
published year: 2002
authors:
  item: Charron C
  item: Vitoux B
  item: Aubry A

Identification of tissue transglutaminase-reactive lysine residues in glyceraldehyde-3-phosphate dehydrogenase.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/11742130
journal: Protein Sci
volume: 11
pages: 137-46
published year: 2002
authors:
  item: Orru S
  item: Ruoppolo M
  item: Francese S
  item: Vitagliano L
  item: Marino G
  item: Esposito C

Hyperthermophilic dehydrogenase enzymes.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/15046583
journal: Biochem Soc Trans
volume: 32
pages: 255-8
published year: 2004
authors:
  item: Littlechild JA
  item: Guy JE
  item: Isupov MN

Brownian dynamics of interactions between glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mutants and F-actin.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/15048777
journal: Biopolymers
volume: 73
pages: 533-41
published year: 2004
authors:
  item: Waingeh VF
  item: Lowe SL
  item: Thomasson KA

ReferenceForCatalyticMechanism
reference index: 5
reference section: p.655-656

ReferenceForCatalyticMechanism
reference index: 6
reference section: p.495-497

ReferenceForCatalyticMechanism
reference index: 9
reference section: p.115-117