D00028
type: Enzyme
cath: Rossmann fold
cath: Dihydrodipicolinate Reductase; domain 2
ec: https://purl.uniprot.org/enzyme/1.3.1.26
csa: 1arz
uniprot: P04036
kegg enzyme: dihydrodipicolinate reductase
kegg enzyme: dihydrodipicolinic acid reductase
kegg enzyme: 2,3,4,5-tetrahydrodipicolinate:NAD(P)+ oxidoreductase
kegg pathway: Lysine biosynthesis
kegg substrate: 2,3,4,5-Tetrahydrodipicolinate
kegg substrate: NADP+
kegg substrate: NAD+
kegg product: 2,3-Dihydrodipicolinate
kegg product: NADH
kegg product: NADPH
kegg product: H+
pdb: 1arzA01
pdb: 1arzB01
pdb: 1arzC01
pdb: 1arzD01
pdb: 1dihA01
pdb: 1druA01
pdb: 1drvA01
pdb: 1drwA01
pdb: 1arzA02
pdb: 1arzB02
pdb: 1arzC02
pdb: 1arzD02
pdb: 1dihA02
pdb: 1druA02
pdb: 1drvA02
pdb: 1drwA02
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1arzA01
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1arzB01
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1arzC01
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1arzD01
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1dihA01
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1druA01
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1drvA01
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1drwA01
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1arzA02
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1arzB02
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1arzC02
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1arzD02
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1dihA02
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1druA02
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1drvA02
pdb bound state: Unbound
  compound: 2,3,4,5-Tetrahydrodipicolinate
  pdb: 1drwA02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1arzA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1arzB01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1arzC01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1arzD01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1dihA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1druA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1drvA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1drwA01
pdb bound state: Unbound
  compound: NADP+
  pdb: 1arzA02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1arzB02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1arzC02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1arzD02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1dihA02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1druA02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1drvA02
pdb bound state: Unbound
  compound: NADP+
  pdb: 1drwA02
pdb bound state: Unbound
  compound: NAD+
  pdb: 1arzA01
pdb bound state: Unbound
  compound: NAD+
  pdb: 1dihA01
pdb bound state: Unbound
  compound: NAD+
  pdb: 1druA01
pdb bound state: Analogue
  compound: NAD+
  pdb: 1drvA01
  ligand het code: A3D
pdb bound state: Analogue
  compound: NAD+
  pdb: 1drwA01
  ligand het code: NHD
pdb bound state: Unbound
  compound: NAD+
  pdb: 1arzA02
pdb bound state: Unbound
  compound: NAD+
  pdb: 1arzB02
pdb bound state: Unbound
  compound: NAD+
  pdb: 1arzC02
pdb bound state: Unbound
  compound: NAD+
  pdb: 1arzD02
active site residues resources: Inhibitors of dihydrodipicolinate reductase, a key enzyme of the diaminopimelate pathway of Mycobacterium tuberculosis.
references: Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase.
references: Expression, purification, and characterization of Escherichia coli dihydrodipicolinate reductase.
references: Interaction of pyridine nucleotide substrates with Escherichia coli dihydrodipicolinate reductase: thermodynamic and structural analysis of binary complexes.
references: Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase in complex with NADH and the inhibitor 2,6-pyridinedicarboxylate.
references: Inhibitors of dihydrodipicolinate reductase, a key enzyme of the diaminopimelate pathway of Mycobacterium tuberculosis.

Rossmann fold
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.40.50.720
catalytic domain: index.html

Dihydrodipicolinate Reductase; domain 2
type: Cath
references: http://www.cathdb.info/version/latest/superfamily/3.30.360.10
catalytic domain: index.html

Lysine biosynthesis
type: KeggPathway
references: KEGG:MAP00300

2,3,4,5-Tetrahydrodipicolinate
type: KeggSubstrate
references: KEGG:C03972
compound type: carboxyl group
compound type: imine group
references: CHEBI:864
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

NADP+
type: KeggSubstrate
references: KEGG:C00006
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:18009
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

NAD+
type: KeggSubstrate
references: KEGG:C00003
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:15846
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

2,3-Dihydrodipicolinate
type: KeggProduct
references: KEGG:C03340
compound type: aromatic ring (with nitrogen atoms)
compound type: carboxyl group
references: CHEBI:18042
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

NADH
type: KeggProduct
references: KEGG:C00004
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:16908
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

NADPH
type: KeggProduct
references: KEGG:C00005
compound type: amide group
compound type: amine group
compound type: nucleotide
references: CHEBI:16474
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

H+
type: KeggProduct
references: KEGG:C00080
compound type: others
references: CHEBI:15378
references: http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi

1arzA02
type: Pdb
references: 1arz
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1arz
catalytic residue: HIS 159;LYS 163

1arzB02
type: Pdb
references: 1arz
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1arz
catalytic residue: HIS 159;LYS 163

1arzC02
type: Pdb
references: 1arz
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1arz
catalytic residue: HIS 159;LYS 163

1arzD02
type: Pdb
references: 1arz
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1arz
catalytic residue: HIS 159;LYS 163

1dihA02
type: Pdb
references: 1dih
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1dih
catalytic residue: HIS 159;LYS 163

1druA02
type: Pdb
references: 1dru
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1dru
catalytic residue: HIS 159;LYS 163

1drvA02
type: Pdb
references: 1drv
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1drv
catalytic residue: HIS 159;LYS 163

1drwA02
type: Pdb
references: 1drw
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: PDB:1drw
catalytic residue: HIS 159;LYS 163

Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS)
medline id: 95200850
references: http://www.ncbi.nlm.nih.gov/pubmed/7893645
journal: Biochemistry
volume: 34
pages: 3502-12
published year: 1995
authors:
  item: Scapin G
  item: Blanchard JS
  item: Sacchettini JC
references: P04036

Expression, purification, and characterization of Escherichia coli dihydrodipicolinate reductase.
type: BibliographicResource
comment: X-ray crystallography
references: http://www.ncbi.nlm.nih.gov/pubmed/7893644
journal: Biochemistry
volume: 34
pages: 3492-501
published year: 1995
authors:
  item: Reddy SG
  item: Sacchettini JC
  item: Blanchard JS
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

Interaction of pyridine nucleotide substrates with Escherichia coli dihydrodipicolinate reductase: thermodynamic and structural analysis of binary complexes.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/8873595
journal: Biochemistry
volume: 35
pages: 13294-302
published year: 1996
authors:
  item: Reddy SG
  item: Scapin G
  item: Blanchard JS
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl

Three-dimensional structure of Escherichia coli dihydrodipicolinate reductase in complex with NADH and the inhibitor 2,6-pyridinedicarboxylate.
type: BibliographicResource
comment: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS)
medline id: 98060760
references: http://www.ncbi.nlm.nih.gov/pubmed/9398235
journal: Biochemistry
volume: 36
pages: 15081-8
published year: 1997
authors:
  item: Scapin G
  item: Reddy SG
  item: Zheng R
  item: Blanchard JS
references: http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl
references: P04036

Inhibitors of dihydrodipicolinate reductase, a key enzyme of the diaminopimelate pathway of Mycobacterium tuberculosis.
type: BibliographicResource
references: http://www.ncbi.nlm.nih.gov/pubmed/11342032
journal: Biochim Biophys Acta
volume: 1545
pages: 67-77
published year: 2001
authors:
  item: Paiva AM
  item: Vanderwall DE
  item: Blanchard JS
  item: Kozarich JW
  item: Williamson JM
  item: Kelly TM

Analogue
compound: 2,3-Dihydrodipicolinate
pdb: 1arzB02
ligand het code: PDC

Analogue
compound: 2,3-Dihydrodipicolinate
pdb: 1arzC02
ligand het code: PDC

Analogue
compound: 2,3-Dihydrodipicolinate
pdb: 1arzD02
ligand het code: PDC

ReferenceForCatalyticMechanism
reference index: 2
reference section: Scheme 3, p.3499-3500

ReferenceForCatalyticMechanism
reference index: 4
reference section: Fig.4, p.15087-15088