The tertiary structure of the N-terminal domains of this enzyme has not been determined yet. Only the catalytic domain of integrase has been solved so far. However, this enzyme seems to be homologous to the structures of its homologue, Pol polyprotein from Moloney murine leukemia virus (M00135 in EzCatDB), except for its N-terminal protease domain. This enzyme catalyzes two successive reactions, hydrolysis (called processing) and transfer (called joining) (see [3]). Although manganese is annotated as the cofactor, other divalent metal such as magnesium, cadmium and zinc can act as the cofactor (see [2] & [3]). However, in the presence of zinc ions, the polynucleotidyl transfer reaction can be inhibited, whilst the endonucleolytic reaction (hydrolysis) can be catalyzed (see [3]).
HIV Type 1 Reverse Transcriptase; Chain A, domain 1
DNA nucleotidyltransferase (RNA-directed)
RNA-dependent deoxyribonucleate nucleotidyltransferase
DNA nucleotidyltransferase (DNA-directed)
deoxyribonucleate nucleotidyltransferase
ASP 64;ASP 121;GLU 157(Magnesium or Manganese binding)
;ASP 121;GLU 157(Magnesium or Manganese binding)
Binding of different divalent cations to the active site of avian sarcoma virus integrase and their effects on enzymatic activity.
Similarities in the HIV-1 and ASV integrase active sites upon metal cofactor binding.
Crystal structure of an active two-domain derivative of Rous sarcoma virus integrase.
High-resolution structure of the catalytic domain of avian sarcoma virus integrase.
The catalytic domain of avian sarcoma virus integrase: conformation of the active-site residues in the presence of divalent cations.
Avian retrovirus U3 and U5 DNA inverted repeats. Role Of nonsymmetrical nucleotides in promoting full-site integration by purified virion and bacterial recombinant integrases.
Structure of the catalytic domain of avian sarcoma virus integrase with a bound HIV-1 integrase-targeted inhibitor.
Structural basis for inactivating mutations and pH-dependent activity of avian sarcoma virus integrase.
Atomic resolution structures of the core domain of avian sarcoma virus integrase and its D64N mutant.
Crystal structures of catalytic core domains of retroviral integrases and role of divalent cations in enzymatic activity.
Tautomers of styrylquinoline derivatives containing a methoxy substituent: computation of their population in aqueous solution and their interaction with RSV integrase catalytic core.
Role of DNA end distortion in catalysis by avian sarcoma virus integrase.
Nucleophile selection for the endonuclease activities of human, ovine, and avian retroviral integrases.
Presteady-state analysis of avian sarcoma virus integrase. I. A splicing activity and structure-function implications for cognate site recognition.
Presteady-state analysis of avian sarcoma virus integrase. II. Reverse-polarity substrates identify preferential processing of the U3-U5 pair.
An amino acid in the central catalytic domain of three retroviral integrases that affects target site selection in nonviral DNA.
Functional oligomeric state of avian sarcoma virus integrase.